A controllable gelatin-based microcarriers fabrication system for the whole procedures of MSCs amplification and tissue engineering

Abstract

Biopolymer microbeads present substantial benefits for cell expansion, tissue engineering, and drug release applications. However, a fabrication system capable of producing homogeneous microspheres with high precision and controllability for cell proliferation, passaging, harvesting and downstream application is limited. Therefore, we developed a co-flow microfluidics-based system for the generation of uniform and size-controllable gelatin-based microcarriers (GMs) for mesenchymal stromal cells (MSCs) expansion and tissue engineering. Our evaluation of GMs revealed superior homogeneity and efficiency of cellular attachment, expansion and harvest, and MSCs expanded on GMs exhibited high viability while retaining differentiation multipotency. Optimization of passaging and harvesting protocols was achieved through the addition of blank GMs and treatment with collagenase, respectively. Furthermore, we demonstrated that MSC-loaded GMs were printable and could serve as building blocks for tissue regeneration scaffolds. These results suggested that our platform held promise for fabrication of uniform GMs with downstream application of MSC culture, expansion, and tissue engineering.

Edible mycelium as proliferation and differentiation support for anchorage-dependent animal cells in cultivated meat production

“…Dissolvable carriers are made of degradable materials (e.g., denatured collagen, cross-linked polygalacturonic acid, alginate, etc.) that can be dissolved once the cells on microcarriers have reached confluency 18 , 19 . However, like the synthetic carriers, dissolving operations add complexity and cost to cell culture and degradation must be carefully controlled to retain cell function.…”

Human Mesenchymal Stem Cells on Size-Sorted Gelatin Hydrogel Microparticles Show Enhanced In Vitro Wound Healing Activities

“…The proliferation and differentiation of various types of cells on gelatin HMPs have been reported previously. These cells include adiposederived stem cells [38,[45][46][47], MC3T3-E1 [48][49][50][51][52], bone-marrow-derived stem cells [53][54][55][56][57], preadipocytes [58], and cardiac progenitor cells [59].…”