A continuous tumor‐cell line from a human lung carcinoma with properties of type II alveolar epithelial cells

Lieber, Michael M.; Todaro, George J.; Smith, Barry T.; Szakal, Andras K.; Nelson‐Rees, Walter A.

doi:10.1002/ijc.2910170110

Cited by 1,110 publications

(687 citation statements)

References 26 publications

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“…The human alveolar carcinoma cell line A549 [14] (RIKEN Cell Bank, Ibaragi, Japan) was maintained in DMEM medium (Invitrogen/Gibco, Carlsbad, CA) containing 10% FCS and antibiotics.…”

Section: Cell Culturementioning

confidence: 99%

A possible link between resveratrol and TGF‐β: Resveratrol induction of TGF‐β expression and signaling

et al. 2008

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Resveratrol, a polyphenolic compound found in the skin of red fruits, exhibits anti-inflammatory, anti-oxidative, and anti-proliferative characteristics. Transforming growth factor-b (TGF-b) is a pleiotropic cytokine that also displays such properties. We therefore hypothesized that there might be a functional link between resveratrol and TGF-b. This study reports that resveratrol increased transcription of the TGF-b2 gene, enhanced the production of TGF-b2 protein, and activated Smad signaling in an autocrine manner in A549 human lung epithelial cell line. Thus, some of the beneficial effects of resveratrol on human health might be mediated, in part, through its effects on TGF-b expression and signaling.

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“…The human alveolar carcinoma cell line A549 [14] (RIKEN Cell Bank, Ibaragi, Japan) was maintained in DMEM medium (Invitrogen/Gibco, Carlsbad, CA) containing 10% FCS and antibiotics.…”

Section: Cell Culturementioning

confidence: 99%

A possible link between resveratrol and TGF‐β: Resveratrol induction of TGF‐β expression and signaling

et al. 2008

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“…This cell line is derived from a lung carcinoma with properties of type II alveolar epithelial cells. 21 The cells were grown on tissue culture dishes in a 1:1 (v/v) mixture of Dulbecco's modified Eagle's minimum essential medium and Ham's F12 medium (DME/F12) supplemented with 5% of fetal bovine serum (FBS). The cell lines were cultured in a CO 2 incubator (5% CO 2 and 95% air).…”

Section: Cells and Cell Culturementioning

confidence: 99%

Overexpression of homeobox geneHOXD3 induces coordinate expression of metastasis-related genes in human lung cancer cells

et al. 2001

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Homeobox-containing genes are expressed in spatiotemporal fashion during embryogenesis and act as master transcription-regulating factors which control the expression of a variety of genes involved in morphogenesis. They are also expressed in a tissue-specific manner in normal adult tissues and appear to give cells spatial information in the maintenance of their architectural integrity. We transfected a HOXD3 class I homeobox-containing gene into human lung cancer A549 cells and investigated alterations in gene expressions and phenotypes related to the maintenance of tissue architecture in HOXD3-overexpressing A549 cells. In the HOXD3-overexpressing cell lines, expression of E-cadherin was lost and plakoglobin was strongly repressed, whereas integrin ␣3 and ␤3 were up-regulated and N-cadherin and integrin ␣4 were newly expressed. Compared with parental and control transfectant lines, the HOXD3-overexpressing cell lines showed highly motile and invasive activity. Blocking experiments using anti-integrin ␤1 and ␤3 suggested that the increased haptotaxis of the HOXD3-overexpressing cells to vitronectin resulted from increased expression and activation of integrin ␣v␤3, and that overexpression of the HOXD3 gene converted the integrin ␤1-dependent haptotaxis to fibronectin into both integrin ␤1-and ␤3-dependent one. HOXD3 overexpression increased production of matrix-degrative enzymes including matrix metalloproteinase-2 and urokinase-plasminogen activator. When the tumor cells were intravenously injected into the tail veins of nude mice, HOXD3 transfectants formed a significantly large number of metastatic foci in lungs compared with the control transfectants. These findings suggest that HOXD3 can act as a metastasis-promoting gene in human lung cancer A549 cells.

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“…CL-185) [21] was cultured (37 mC, 5 % CO # ) in Dulbecco's modified Eagle's medium (Gibco-BRL, Grand Island, NY, U.S.A.) with 10 % heat-inactivated calf serum, 2 mM -glutamine and 500 units\ml penicillin and streptomycin. For all experiments, cells were used between passages 80 and 120.…”

Section: Cell Culturementioning

confidence: 99%

Biological effects of menadione photochemistry: effects of menadione on biological systems may not involve classical oxidant production

McCormick

Denning

Reszka

et al. 2000

Biochemical Journal

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Because cell-mediated reduction of menadione leads to the generation of reactive oxygen species (ROS), this quinone is widely used to investigate the effects of ROS on cellular functions. We report that A549 human lung epithelial cells exposed to menadione demonstrate a dose-dependent increase in both intracellular calcium ([Ca# + ] i ) and ROS formation. The concentrations of menadione required to initiate these two events are markedly different, with ROS detection requiring higher levels of menadione. Modulators of antioxidant defences (e.g. buthionine sulphoximine, 3-amino-1,2,4-triazole) have little effect on the [Ca# + ] i response to menadione, suggesting that ROS formation does not account for menadione-dependent alterations in [Ca# + ] i . Additional evidence suggests that menadione photochemistry may be responsible for the observed [Ca# + ] i effects. Specifically : (a) EPR studies with the spin trap 5,5-dimethyl-1-pyrroline-Noxide (DMPO) show that light exposure (maximum effect at

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A continuous tumor‐cell line from a human lung carcinoma with properties of type II alveolar epithelial cells

Cited by 1,110 publications

References 26 publications

A possible link between resveratrol and TGF‐β: Resveratrol induction of TGF‐β expression and signaling

A possible link between resveratrol and TGF‐β: Resveratrol induction of TGF‐β expression and signaling

Overexpression of homeobox geneHOXD3 induces coordinate expression of metastasis-related genes in human lung cancer cells

Biological effects of menadione photochemistry: effects of menadione on biological systems may not involve classical oxidant production

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