A continuous spectrophotometric assay for the activation of plant NAD kinase by calmodulin, calcium(II), and europium(III) ions

Amann, Barbara; Mulqueen, Paul; Horrocks, William DeW.

doi:10.1016/0165-022x(92)90015-3

Cited by 8 publications

(5 citation statements)

References 52 publications

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“…Even though lanthanides are widely regarded as biologically non-essential elements, low concentrations of at least some REEs result in improved plant growth and yields. A similar effect could also be observed for other photosynthetic organisms, such as the green alga Desmodesmus quadricauda [ 38 ], and the increase in the photosynthetic rate observed in the presence of Ln has been ascribed to the replacement of the central Mg ion of chlorophyll by REEs [ 25 , 26 ]. While increased crop yields already represent a reason to supplement soil with REEs [ 5 ], their role in the phytoremediation of lanthanides from soil and water also deserves attention [ 39 ].…”

Section: Discussionsupporting

confidence: 58%

“…In addition, several reports exist that show that many non-related enzymes retain their abilities upon binding of Ln ions. This includes very well-studied Ca-binding proteins such as calmodulin from pea seedlings [ 26 ] and α-type phospholipase D from white cabbage [ 27 ], as well as tobacco Mg-binding proteins, such as ribulose bi-phosphate carboxylase [ 28 ] and Mg-ATPase [ 29 ]. To the best of our knowledge, we report here for the first time an example of a new class of enzymes, an E3 ligase that binds some Ln ions, i.e., La 3+ and Tb 3+ .…”

Section: Discussionmentioning

confidence: 99%

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E3 Ubiquitin Ligase SPL2 Is a Lanthanide-Binding Protein

Tracz

Górniak

Szczepaniak

et al. 2021

IJMS

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The SPL2 protein is an E3 ubiquitin ligase of unknown function. It is one of only three types of E3 ligases found in the outer membrane of plant chloroplasts. In this study, we show that the cytosolic fragment of SPL2 binds lanthanide ions, as evidenced by fluorescence measurements and circular dichroism spectroscopy. We also report that SPL2 undergoes conformational changes upon binding of both Ca2+ and La3+, as evidenced by its partial unfolding. However, these structural rearrangements do not interfere with SPL2 enzymatic activity, as the protein retains its ability to auto-ubiquitinate in vitro. The possible applications of lanthanide-based probes to identify protein interactions in vivo are also discussed. Taken together, the results of this study reveal that the SPL2 protein contains a lanthanide-binding site, showing for the first time that at least some E3 ubiquitin ligases are also capable of binding lanthanide ions.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

E3 Ubiquitin Ligase SPL2 Is a Lanthanide-Binding Protein

Tracz

Górniak

Szczepaniak

et al. 2021

IJMS

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“…What is the explanation of these effects? Some lanthanoids are able to replace endogenous calcium ions in some enzymes, such as peroxidase in horseradish (Morishima et al, 1986) or calmodulin in pea seedlings (Amann et al, 1992) and Amaranthus caudatus seedlings with retaining its activity. In addition, Nd(III) is able to replace Ca(II) under conditions of Ca deficiency (Wei et al, 2001;Zhang and Shan, 2001).…”

Section: Other Rare Heavy Metals and Metalloidsmentioning

confidence: 99%

Uncommon Heavy Metals, Metalloids and Their Plant Toxicity: A Review

Babula

Adam

Opatřilová

et al. 2009

Sustainable Agriculture Reviews

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Heavy metals represent a group of dangerous pollutants, to which is paid close attention. Many heavy metals are essential as important constituents of pigments and enzymes-mainly zinc, nickel, and copper. However, all metals, especially cadmium, lead, mercury, and copper, are toxic in high concentrations because they disrupt enzyme functions, replacing essential metals in pigments or producing reactive oxygen species. The toxicity of less common heavy metals and metalloids, such as thallium, arsenic, chromium, antimony, selenium, and bismuth has been investigated. Here we review the phytotoxicity of thallium, chromium, antimony, selenium, bismuth; and other rare heavy metals and metalloids such as

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“…Eu 3+ has already been shown to be able to replace Ca 2+ in some physiological mechanisms due to similarities in their ionic radii (Amann et al, 1992;Zeng et al, 2003;Shtangeeva and Ayrault, 2007). Therefore, the Eu/Eu* 680 increased value in Do OLn may be due to the partial involvement of Eu in the Ca-dedicated biochemical pathways described above, which would lead to an anomalous accumulation of Eu in respect to the other REE in Douglas-fir litter.…”

Section: Behaviour Of Ca and Eu During Litter Degradation 660mentioning

confidence: 96%

Dynamics of Rare Earths and associated major and trace elements during Douglas-fir (Pseudotsuga menziesii) and European beech (Fagus sylvatica L.) litter degradation

Montemagno

Hissler

Bense

et al. 2021

Preprint

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Abstract. Given the diverse physico-chemical properties of elements, we hypothesize that their incoherent distribution across the leaf tissues, combined with the distinct resistance to degradation that each tissue exhibits, leads to distinct turnover rates between elements. Moreover, litter layers of different ages produce diverse chemical signatures in solution during the wet degradation. To verify our hypothesis, Na, K, Mg, Mn, Ca, Pb, Al and Fe were analysed together with the Rare Earth Elements (REE) in the solid fractions and in the respective leachates of fresh leaves and different humus layers of two forested soils developed under Pseudotsuga menziesii and Fagus sylvatica L. trees. The results from the leaching experiment were also compared to the in situ REE composition of the soil solutions to clarify the impact that the litter degradation processes may have on soil solution chemical compositions. Our results clearly show that REE, Al, Fe and Pb were preferentially retained in the solid litter material, in comparison to the other cations, and that their concentrations increased over time during the litter degradation. Accordingly, different litter fractions produced different yields of elements and REE patterns in the leachates, indicating that the tree species and the age of the litter play a role in the chemical release during the degradation. In particular, the evolution of the REE patterns according to the age of the litter layers allowed us to deliver new findings on REE fractionation and mobilization during litter degradation. In particular, the LaN/YbN ratio highlights differences in litter degradation intensity between both tree species, which was not shown with major cations. We finally showed the primary control effect that litter degradation can have on the REE composition of the soil solution, which presents a positive Ce anomaly associated with the dissolution and/or transportation of Ce-enriched MnO2 particles accumulated onto the surface of the old litter due to white fungi activity. Similar MREE and HREE enrichments were also found in the leachates and the soil solution, probably due to their higher affinity to the organic acids, which represent the primary products from the organic matter degradation.

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A continuous spectrophotometric assay for the activation of plant NAD kinase by calmodulin, calcium(II), and europium(III) ions

Cited by 8 publications

References 52 publications

E3 Ubiquitin Ligase SPL2 Is a Lanthanide-Binding Protein

E3 Ubiquitin Ligase SPL2 Is a Lanthanide-Binding Protein

Uncommon Heavy Metals, Metalloids and Their Plant Toxicity: A Review

Dynamics of Rare Earths and associated major and trace elements during Douglas-fir (Pseudotsuga menziesii) and European beech (Fagus sylvatica L.) litter degradation

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