A Continuous Assay Set to Screen and Characterize Novel Protein N-Acetyltransferases Unveils Rice General Control Non-repressible 5-Related N-Acetyltransferase2 Activity
Abstract:Protein N-acetyltransferases (NATs) belong to the general control non-repressible 5 (Gcn5)-related N-acetyltransferases (GNATs) superfamily. GNATs catalyze the transfer of acetyl from acetyl-CoA to the reactive amine moiety of a wide range of acceptors. NAT sequences are difficult to distinguish from other members of the GNAT superfamily and there are many uncharacterized GNATs. To facilitate the discovery and characterization of new GNATs, we have developed a new continuous, non-radioactive assay. This assay … Show more
“…These GNATs are also close homologs to the plant NAA90s, one of the two NatG subgroups ( Giglione and Meinnel, 2021 ). In the absence of reverse genetics tools for Paulinellidae, alternative approaches to characterize and confirm Paulinella NatG activities would be interesting to implement ( Asensio et al, 2022 ). One conclusion therefore is that NTA in Paulinella spp.…”
Mass spectrometry analysis of protein processing in a photosynthetic rhizarian amoeba, Paulinella chromatophora, suggests a major trafficking route from the cytosol to the chromatophore via the Golgi.
“…These GNATs are also close homologs to the plant NAA90s, one of the two NatG subgroups ( Giglione and Meinnel, 2021 ). In the absence of reverse genetics tools for Paulinellidae, alternative approaches to characterize and confirm Paulinella NatG activities would be interesting to implement ( Asensio et al, 2022 ). One conclusion therefore is that NTA in Paulinella spp.…”
Mass spectrometry analysis of protein processing in a photosynthetic rhizarian amoeba, Paulinella chromatophora, suggests a major trafficking route from the cytosol to the chromatophore via the Golgi.
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