Background: -Telemedicine is the delivery of healthcare services at a distance. Despite it is an emerging and infancy technology in a developing country, utilizing the technology for delivering healthcare services is low in Ethiopia. To maximize and facilitate Telemedicine adoption it prominently requires information about the knowledge and awareness of telemedicine services among health professionals.Methods: - An institution-based cross-sectional study design was conducted among 423 health professionals working at Amhara Region Referral Hospitals from February 12-March 20/2020. The data were collected using a self-administered structured questionnaire. Desk review was done to assure the data quality and then data was entered into EPI INFO version 7 and exported into SPSS version 20 for statistical analysis. Descriptive statistics, bivariable and multivariable logistic regression analyses were done. The odds ratio with a 95% confidence interval (95% CI) was used to identify associated factors.Result: About 56% of health professionals had good knowledge and 57.4% had high awareness towards telemedicine services with a 411 (97.2%) response rate. Information sharing culture [AOR=3.01, 95% CI: 1.89, 4.80], having IT support staff [AOR=1.87, 95%CI: 1.06, 3.29], internet as information source [AOR=1.80, 95%CI:1.1, 2.94], awareness [AOR=1.35,95% CI: 1.03, 2.40], being male [AOR=1.73,95% CI:1.06, 2.81] were significantly associated with the knowledge of the respondents towards telemedicine services and telemedicine training [AOR= 2.33, 95% CI: 1.15, 4.72] and computer accessibility in their hospitals [AOR= 1.54, 95% CI: 1.01, 2.35] were significantly associated with the awareness of the respondents towards telemedicine services.Conclusion: More than half of the respondents had good knowledge and high awareness of telemedicine services. Information sources, having IT support staff, information sharing culture, gender and awareness were significant factors for the knowledge of telemedicine service, and telemedicine training and computer access were significant factors of awareness of health professionals towards telemedicine services. Therefore Appropriate and regular awareness creation training of telemedicine systems should be given to the health professional.