A Contact Lens-Based Technique for Expansion and Transplantation of Autologous Epithelial Progenitors for Ocular Surface Reconstruction

Girolamo, Nick Di; Bosch, Martina M.; Zamora, Katherine V; Coroneo, Minas T.; Wakefield, Denis; Watson, Stephanie

doi:10.1097/tp.0b013e3181a4bbf2

Cited by 159 publications

(102 citation statements)

References 39 publications

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“…The procedure was found to improve or to restore vision in a majority of the cases, and subsequent studies from other groups confirmed the potential of this new therapeutic approach [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 77%

Trypsin for Dissociation of Limbal Cells for Engineering of Grafts May Induce DNA Strand Breaks in the Harvested Cells

2013

J Ocul Biol

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Section: Introductionmentioning

confidence: 77%

Trypsin for Dissociation of Limbal Cells for Engineering of Grafts May Induce DNA Strand Breaks in the Harvested Cells

2013

J Ocul Biol

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“…24,25 For the past decade, it has been demonstrated that CLs provide an adequate reservoir for drugs and/or cells to adjust the environment of the cornea. It has been shown by DiGirolamo et al 25 that soft CLs can be used as a carrier for autologous epithelial cells to aid ocular rehabilitation in patients with unilateral LSCD, although this method is impossible with bilateral LSCD. Scleral lenses have been used by Espandar et al 24 to correct the inflammatory environment in the event of alkali burn in rabbits.…”

Section: Discussionmentioning

confidence: 99%

“…22,23 Scleral lenses have been used as carriers of adipose-derived stem cells in a rabbit corneal epithelium repair model 24 and soft CLs have been used to deliver expanded epithelial cells to the cornea. 25 These characteristics make CLs ideal candidate carriers for the transfer of DPSCs or transdifferentiated DPSCs to an injured cornea.…”

mentioning

confidence: 99%

Regeneration of Corneal Epithelium With Dental Pulp Stem Cells Using a Contact Lens Delivery System

Kushnerev

Shawcross

Sothirachagan

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…However, transfer of cells from the lens to the corneal surface was not conclusively demonstrated. 34 We used the same commercial siloxane-hydrogel contact lenses as control surfaces. We confirmed that corneal epithelium can be cultured on siloxane-hydrogel contact lenses.…”

Section: Discussionmentioning

confidence: 99%

“…As expected, none of the explants remained attached on OCT-only surfaces. The commercially available siloxane hydrogel contact lenses (Focus Night and Day lens [CIBA Vision]), which were used previously for the culture and transplantation of the corneal epithelium in human patients, 34 were used as positive controls and showed about 81% explant attachment.…”

Section: Polymer-coated Contact Lens For Corneal Epithelial Cell Tranmentioning

confidence: 99%

Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

Brown

Low

Mariappan

et al. 2014

Tissue Engineering Part A

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Extensive damage to the limbal region of the cornea leads to a severe form of corneal blindness termed as limbal stem cell deficiency (LSCD). Whereas most cases of corneal opacity can be treated with full thickness corneal transplants, LSCD requires stem cell transplantation for successful ocular surface reconstruction. Current treatments for LSCD using limbal stem cell transplantation involve the use of murine NIH 3T3 cells and human amniotic membranes as culture substrates, which pose the threat of transmission of animal-derived pathogens and donor tissue-derived cryptic infections. In this study, we aimed to produce surface modified therapeutic contact lenses for the culture and delivery of corneal epithelial cells for the treatment of LSCD. This approach avoids the possibility of suture-related complications and is completely synthetic. We used plasma polymerization to deposit acid functional groups onto the lenses at various concentrations. Each surface was tested for its suitability to promote corneal epithelial cell adhesion, proliferation, retention of stem cells, and differentiation and found that acid-based chemistries promoted better cell adhesion and proliferation. We also found that the lenses coated with a higher percentage of acid functional groups resulted in a higher number of cells transferred onto the corneal wound bed in rabbit models of LSCD. Immunohistochemistry of the recipient cornea confirmed the presence of autologous, transplanted 5-bromo-2¢-deoxyuridine (BrdU)-labeled cells. Hematoxylin staining has also revealed the presence of a stratified epithelium at 26 days post-transplantation. This study provides the first evidence for in vivo transfer and survival of cells transplanted from a contact lens to the wounded corneal surface. It also proposes the possibility of using plasma polymer-coated contact lenses with high acid functional groups as substrates for the culture and transfer of limbal cells in the treatment of LSCD.

show abstract

A Contact Lens-Based Technique for Expansion and Transplantation of Autologous Epithelial Progenitors for Ocular Surface Reconstruction

Abstract: Ex vivo expansion of ocular surface epithelium in the presence of autologous serum and transplantation with the aid of a soft CLs is a promising new technique capable of achieving ocular surface rehabilitation.

Cited by 159 publications

References 39 publications

Trypsin for Dissociation of Limbal Cells for Engineering of Grafts May Induce DNA Strand Breaks in the Harvested Cells

Trypsin for Dissociation of Limbal Cells for Engineering of Grafts May Induce DNA Strand Breaks in the Harvested Cells

Regeneration of Corneal Epithelium With Dental Pulp Stem Cells Using a Contact Lens Delivery System

Plasma Polymer-Coated Contact Lenses for the Culture and Transfer of Corneal Epithelial Cells in the Treatment of Limbal Stem Cell Deficiency

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