A new isolated Bacillus strain was cultivated continuously at 68 "C and pH 3 for a long time. Extractable lipids represent about 3.6% ofcell dry weight and are made up of 18% neutral lipids, 39.3% glycolipids, and 42% acidic lipids. Main components of the fatty acid fraction are o-cyclohexylundecanoic acid and w-cyclohexyltridecanoic acid, respectively. MK-7 and hop-22(29)-ene are components of the unsaponifiable fraction. The glycolipid fraction contains the pentacyclic triterpenoid tetrahydroxybacteriohopane. Based on the thermoacidophilic growth conditions, the morphologic and physiological properties, and the nature of lipids, it can assumed that the new isolated strain belongs to the species Bacillus acidocaldariiis.Microorganisms in extreme environments contain unusual lipids which are an adaptation to the unusual environment, especially high temperature [I]. Predominantly most bacilli possess branched-chain fatty acids which are derived biosynthetically from precursors of the branched-chain amino acids valine, leucine, and isoleucine [2]. A group of thermoacidophilic bacilli contains as main components w-cyclohexyl fatty acids with 17 and 19 C-atoms biosynthesized via the shikimate pathway [3]. The occurence of o-cycloheptyl fatty acids with 18 and 20 C-atoms is described in a moderately thermoacidophilic bacillus [4].It has been demonstrated that B. acidocaldarius contains pentacyclic triterpenes [5]. The content of extended hopanoids increased strongly with increasing temperature and