A conserved odorant binding protein is required for essential amino acid detection in Drosophila

Fraichard, Stéphane; Chauvel, Isabelle; Poirier, Nicolas; Delompré, Thomas; Neiers, Fabrice; Tanimura, Teiichi; Ferveur, Jean‐François; Rihani, Karen

doi:10.1038/s42003-019-0673-2

Cited by 24 publications

(28 citation statements)

References 59 publications

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“…The DsOBP69a and DsOBP76a proteins not only play a crucial role in olfactory, but also play an important role in taste. This is consistent with the researches that OBP have an important role in non-olfactory, such as taste (Jeong et al, 2013;Rihani et al, 2019). In this study, berberine chloride and coumarin have a good affinity with the DsOBP69a and DsOBP76a proteins.…”

Section: Discussionsupporting

confidence: 92%

“…The roles of DsOBP69a and DsOBP76a in the taste of D. suzukii remain to be further studied. The D. melanogaster OBP19b protein, a taste-expressed OBP, not only is detected some bitter compounds, such as berberine, but also plays a crucial role in the detection of indispensable nutrients, such as L-phenylalanine (Rihani et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…The odorant molecules bind to the odorant binding proteins (OBPs) in the sensory lymph, and they are transported to the sensory neuron dendrites where they bind to the receptors, stimulating the neuron to transmit sensory signals to the central nervous system (Leal, 2013;Kohl et al, 2015;Joseph and Carlson, 2015). Some D. suzukii OBPs, like D. melanogaster OBPs (Hekmat-Scafe et al, 2002;Larter et al 2016;Rihani et al, 2019), are expressed in the olfactory sensilla on the antenna and taste sensilla of the labellum and leg (Ahn et al, 2020;Crava et al, 2019;Ramasamy, 2016). D. melanogaster perception of cVA requires OBP76a (also known as Lush) to promote cVA transport for binding through OR67d and SNMP proteins (Laughlin et al, 2008;Gomez-Diaz et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Zhan¹,

Dewer

et al. 2021

Heliyon

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The fruit fly Drosophila suzukii is a fruit crop pest that causes a severe economic threat to soft summer fruit worldwide. The male sex pheromone, cis-vaccenyl acetate (cVA) has multiple functions in intra-species communication in Drosophila melanogaster, which is required in male to suppress male-male courtship. D. suzukii males do not produce cVA; however, the odorant receptor for cVA (Or67d) is still functional. The lack of cVA in D. suzukii casts the question of whether this pheromone might have been replaced by another compound similar to cVA that disrupts mating in D. suzukii. In order to address this question, we cloned two D. suzukii adult antenna-specific odorant-binding proteins (OBPs) DsOBP69a and DsOBP76a and aligned with their D. melanogaster orthologues. Moreover, we examined the binding properties of the newly identified recombinant proteins against 26 potential ligands including cVA, using the fluorescence-based ligand binding assay. The alignment showed that DsOBP69a and DsOBP76a, have six conserved cysteines and belong to the classic OBP family. Furthermore, our results revealed that cVA did not bind to DsOBP69a or DsOBP76a proteins. Interestingly, the floral odorant β-ionone and the bitter substance berberine chloride and coumarin displayed high binding ability. It is also worth noting that DsOBP69a and DsOBP76a have different affinities to (Z)-7-Tricosene that may reflect different functional roles. These findings suggest that DsOBP69a and DsOBP76a are potentially involved in olfaction and gustation of D. suzukii.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Zhan¹,

Dewer

et al. 2021

Heliyon

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“…OBPs may also be involved in gain control of the olfactory sensory neuron (OSN) response [43][44][45]. Furthermore, OBPs play a role in social interactions [46], essential amino acid detection [47] for taste and the deactivation kinetics of signal transduction [45]. In insect vectors like mosquitos, OBPs were found to be important for the detection of oviposition attractants [48] and other general odor compounds [48][49][50].…”

Section: Introductionmentioning

confidence: 99%

Antennal Enriched Odorant Binding Proteins Are Required for Odor Communication in Glossina f. fuscipes

et al. 2021

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Olfaction is orchestrated at different stages and involves various proteins at each step. For example, odorant-binding proteins (OBPs) are soluble proteins found in sensillum lymph that might encounter odorants before reaching the odorant receptors. In tsetse flies, the function of OBPs in olfaction is less understood. Here, we investigated the role of OBPs in Glossina fuscipes fuscipes olfaction, the main vector of sleeping sickness, using multidisciplinary approaches. Our tissue expression study demonstrated that GffLush was conserved in legs and antenna in both sexes, whereas GffObp44 and GffObp69 were expressed in the legs but absent in the antenna. GffObp99 was absent in the female antenna but expressed in the male antenna. Short odorant exposure induced a fast alteration in the transcription of OBP genes. Furthermore, we successfully silenced a specific OBP expressed in the antenna via dsRNAi feeding to decipher its function. We found that silencing OBPs that interact with 1-octen-3-ol significantly abolished flies’ attraction to 1-octen-3-ol, a known attractant for tsetse fly. However, OBPs that demonstrated a weak interaction with 1-octen-3-ol did not affect the behavioral response, even though it was successfully silenced. Thus, OBPs’ selective interaction with ligands, their expression in the antenna and their significant impact on behavior when silenced demonstrated their direct involvement in olfaction.

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“…Figures were generated using the interactive Tree of Life (iTOL) v4 software36 (https ://itol.embl.de/). (c) In D. melanogaster, Obp19b is involved with taste perception of amino acids44 . The chemosensory role of the remaining DE OBPs is largely unknown.…”

mentioning

confidence: 99%

Physiological and molecular correlates of the screwworm fly attraction to wound and animal odors

Hickner

Mittapalli

Subramoniam

et al. 2020

Sci Rep

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The screwworm fly, Cochliomyia hominivorax (Coquerel), was successfully eradicated from the United States by the sterile insect technique (SIT). However, recent detection of these flies in the Florida Keys, and increased risk of introductions to the other areas warrant novel tools for management of the flies. Surveillance, a key component of screwworm control programs, utilizes traps baited with rotting liver or a blend of synthetic chemicals such as swormlure-4. In this work, we evaluated the olfactory physiology of the screwworm fly and compared it with the non-obligate ectoparasitic secondary screwworm flies, C. macellaria, that invade necrotic wound and feed on dead tissue. These two species occur in geographically overlapping regions. C. macellaria, along with other blowflies such as the exotic C. megacephala, greatly outnumber C. hominivorax in the existing monitoring traps. Olfactory responses to swormlure-4 constituents between sex and mating status (mated vs unmated) in both species were recorded and compared. Overall, responses measured by the antennograms offered insights into the comparative olfactory physiology of the two fly species. We also present detailed analyses of the antennal transcriptome by RNA-Sequencing that reveal significant differences between male and female screwworm flies. The differential expression patterns were confirmed by quantitative PCR. Taken together, this integrated study provides insights into the physiological and molecular correlates of the screwworm’s attraction to wounds, and identifies molecular targets that will aid in the development of odorant-based fly management strategies.

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A conserved odorant binding protein is required for essential amino acid detection in Drosophila

Cited by 24 publications

References 59 publications

Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Identification and functional characterization of odorant-binding proteins 69a and 76a of Drosophila suzukii

Antennal Enriched Odorant Binding Proteins Are Required for Odor Communication in Glossina f. fuscipes

Physiological and molecular correlates of the screwworm fly attraction to wound and animal odors

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