A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants

Guo, Song; Wong, Sek‐Man

doi:10.3390/v12020195

Cited by 10 publications

(8 citation statements)

References 46 publications

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“…The expression of many CXE genes is upregulated under abiotic stress, such as alkaline stress ( Rui et al., 2022 ) and V. flexuosa infection ( Islam and Yun, 2016 ). In our study, the expression levels of NtCXE6 and NtCXE7 were significantly increased after CMV infection, which is consistent with NbCXE expression in tobacco infected with TMV ( Guo and Wong, 2020 ). Overexpression of the AtCXE8 gene in A. thaliana enhances resistance to Botrytis cinerea ( Lee et al., 2013 ), and in our study, NtCXE14 expression was significantly increased upon inoculation with P. nicotianae .…”

Section: Discussionsupporting

confidence: 88%

“…CXEs also participate in the regulation of plant tolerance to both biotic and abiotic stresses. For example, GBCXE49 regulates the tolerance of cotton to alkali stress ( Rui et al., 2022 ); AtCXE8 enhances plant resistance to gray mold, with the knockout of this gene increasing plant susceptibility ( Lee et al., 2013 ); and NbCXE is a novel resistance-related gene that inhibits the accumulation of tobacco mosaic virus (TMV) in tobacco plants ( Guo and Wong, 2020 ). CXEs participate in the activation of plant hormone-signaling substances, regulating IAA metabolism in immature maize endosperm tissues ( Kowalczyk et al., 2003 ).…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive analysis of the carboxylesterase gene reveals that NtCXE22 regulates axillary bud growth through strigolactone metabolism in tobacco

Wang

Xie²,

Xu³

et al. 2022

Front. Plant Sci.

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Carboxylesterases (CXE) are a class of hydrolytic enzymes with α/β-folding domains that play a vital role in plant growth, development, stress response, and activation of herbicide-active substances. In this study, 49 Nicotiana tabacum L. CXE genes (NtCXEs) were identified using a sequence homology search. The basic characteristics, phylogenetic evolution, gene structure, subcellular location, promoter cis-elements, and gene expression patterns of the CXE family were systematically analyzed. RNA-seq data and quantitative real-time PCR showed that the expression level of CXEs was associated with various stressors and hormones; gene expression levels were significantly different among the eight tissues examined and at different developmental periods. As a new class of hormones, strigolactones (SLs) are released from the roots of plants and can control the germination of axillary buds.NtCXE7, NtCXE9, NtCXE22, and NtCXE24 were homologous to Arabidopsis SLs hydrolase AtCXE15, and changes in their expression levels were induced by topping and by GR24 (a synthetic analogue of strigolactone). Further examination revealed that NtCXE22-mutant (ntcxe22) plants generated by CRISPR-Cas9 technology had shorter bud outgrowth with lower SLs content. Validation of NtCXE22 was also performed in NtCCD8-OE plants (with fewer axillary buds) and in ntccd8 mutant plants (with more axillary buds). The results suggest that NtCXE22 may act as an efficient SLs hydrolase and affects axillary bud development, thereby providing a feasible method for manipulating endogenous SLs in crops and ornamental plants.

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Section: Discussionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of the carboxylesterase gene reveals that NtCXE22 regulates axillary bud growth through strigolactone metabolism in tobacco

Wang

Xie²,

Xu³

et al. 2022

Front. Plant Sci.

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“…Of these 26 DEPs, two proteins are highly associated with resistance to pathogen in plant, including carboxylesterase and GDSL esterase/lipase Carboxylesterases (CXEs) have been implicated in plant defense. A conserved NbCXE inhibited accumulation of Tobacco mosaic virus (TMV) in Nicotiana benthamiana, which enhanced plant resistance [82]. Constitutive expression of PepEST, a fungus-inducible carboxylesterase in peper (Capsicum annuum) increased resistance against the hemibiotrophic anthracnose fungus (Colletotrichum gloeosporioides) [83].…”

Section: Deps Associated Resistance In Pahangmentioning

confidence: 99%

Proteomic analysis of banana xylem sap provides insight into resistant mechanisms to Fusarium oxysporum f. sp. cubense Tropical Race 4

Zhang¹,

Liu²,

Li³

et al. 2020

Preprint

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Background Fusarium wilt is a destructive soilborne disease of banana caused by Fusarium oxysporum f. sp. cubense ( Foc ), especially Tropical Race 4 (TR4), which is a xylem-invading fungus. It was evident that xylem sap contained macromolecules, such as proteins, involved in disease-resistance processes. However, there is no research to analyze changes in banana xylem sap proteins response to TR4 to date. Methods To gain an integrated understanding of differential protein expression in banana xylem sap during TR4 infection, we performed a comparative proteomic analysis of xylem sap in resistant ‘Pahang’ and susceptible ‘Brazilian’ bananas inoculated with TR4. Results A total of 1036 proteins were detected in xylem sap of both bananas, among which some proteins are involved in ‘signal transduction’, ‘environmental adaptation’, ‘biosynthesis of secondary metabolites’ and ‘lipid metabolism’, indicating that xylem sap contained defense-related proteins. A number of 129 differential expression proteins (DEPs) were identified in 4 possible pairs between resistant and susceptible tested combinations. Of these DEPs, hypersensitive-induced response protein 1 (HIR1), E3 ubiquitin ligase (E3) might play negative roles in ‘Pahang’ response to TR4 attack, whereas chalcone isomerase (CHI), glycine-rich RNA-binding protein (GRP), carboxylesterase (CXE) and GDSL lipase (GLIP) might play positive roles in ‘Pahang’ defense against TR4 infection. Conclusions Banana xylem sap contained defense-related proteins, among which HIRP1, E3, CHI, GRP, CXE and GLIP involved in banana defense against TR4. To our knowledge, this is first report to analyze changes in banana xylem sap proteins response to TR4, which help us to explore molecular mechanisms of banana resistant to Fusarium wilt.

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“…Carboxylesterases (CXEs) are implicated in plant defense. A conserved NbCXE inhibited accumulation of Tobacco mosaic virus (TMV) in Nicotiana benthamiana, enhanced plant resistance [74] while constitutive expression of PepEST, a fungus-inducible carboxylesterase in pepper (Capsicum annuum) increased resistance against the hemibiotrophic anthracnose fungus (Colletotrichum gloeosporioides) [75]. In the present study, a CXE protein (Ma06_t34160) was speci cally in higher abundance in TR4 inoculated Pahang compared to Brazilian.…”

Section: Pahang Speci C Reaction During Tr4 Infectionmentioning

confidence: 52%

Proteomic Analysis of Banana Vascular Sap Provides Insight Into Resistance Mechanisms to Fusarium Oxysporum F. Sp. Cubense Tropical Race 4

Zhang¹,

Liu²,

Li³

et al. 2021

Preprint

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Background: Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is the causal agent of Fusarium wilt, and is the most destructive soil-borne and vascular invasive fungus of banana. The sap circulating in vascular cells transports proteins including those that might be involved in disease-resistance processes. However, there is no research to analyze changes in banana vascular sap protein response to TR4 to date. Results: To gain an integrated understanding of differential protein abundance in banana vascular sap during TR4 infection, we performed a comparative proteomic analysis of vascular sap of the resistant ‘Pahang’ and the susceptible ‘Brazilian’ bananas inoculated with TR4. We identified 129 differential expression proteins (DEPs) between resistant and susceptible tested combinations. Of these DEPs, hypersensitive-induced response protein 1 (HIR1) and E3 ubiquitin ligase (E3) decreased in abundance in Pahang with no change in Brazilian under TR4 infection; chalcone isomerase (CHI) and glycine-rich RNA-binding protein (GRP) increased in abundance in Pahang but no significant changes in Brazilian under TR4 infection; carboxylesterase (CXE) and GDSL lipase (GLIP) were specifically in higher abundance in Pahang response to TR4 compared to that of Brazilian. It suggested that these proteins played important roles in bananas against TR4. Conclusions: Our study identified 129 DEPs in vascular sap between resistant and susceptible tested combinations. Of which, HIR1, E3, CHI, GRP, CXE and GLIP played important roles in bananas response to TR4. To our knowledge, this is first report to analyze changes in banana vascular sap proteins in response to TR4, which help us to explore the molecular mechanisms of banana defense to Fusarium wilt.

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A Conserved Carboxylesterase Inhibits Tobacco mosaic virus (TMV) Accumulation in Nicotiana benthamiana Plants

Cited by 10 publications

References 46 publications

Comprehensive analysis of the carboxylesterase gene reveals that NtCXE22 regulates axillary bud growth through strigolactone metabolism in tobacco

Comprehensive analysis of the carboxylesterase gene reveals that NtCXE22 regulates axillary bud growth through strigolactone metabolism in tobacco

Proteomic analysis of banana xylem sap provides insight into resistant mechanisms to Fusarium oxysporum f. sp. cubense Tropical Race 4

Proteomic Analysis of Banana Vascular Sap Provides Insight Into Resistance Mechanisms to Fusarium Oxysporum F. Sp. Cubense Tropical Race 4

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