A conscious mouse model of gastric ileus using clinically relevant endpoints

Firpo, Matthew A.; Rollins, Michael D.; Szabó, Anikó; Gull, Justin D; Jackson, Jean; Shao, Yuanlin; Glasgow, Robert E.; Mulvihill, Sean J.

doi:10.1186/1471-230x-5-18

Cited by 12 publications

(8 citation statements)

References 38 publications

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“…The PR method was used by multiple previous studies (Suchitra et al ., , de Rosalmeida et al . ; Firpo et al ., ; Qiu et al ., and Palheta et al ., ). It was validated by Bennink et al .…”

Section: Methodsmentioning

confidence: 99%

Black Tea Extract and its Thearubigins Relieve the Sildenafil‐Induced Delayed Gut Motility in Mice: A Possible Role of Nitric Oxide

Murad

Abdallah

2014

Phytotherapy Research

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In this study we hypothesize that a standardized black tea aqueous extract (BTE) and thearubigins, its main polyphenolic pigments, will improve sildenafil-induced delay in gastric emptying (GE) and small intestinal transit (SIT) in mice. Twenty groups of mice (n = 8) were given a phenol red meal, and three sets of experiments were performed. In the first and second sets, effects of different concentrations of BTE, thearubigins (TRs), and sildenafil (SLD), alone and in combinations, on GE and SIT were measured. In the third set, influence of nω -Nitro-l-arginine methyl ester hydrochloride (l-NAME) pretreatment on effects of these treatments was tested. Black tea extract (3% and 4.5%) and thearubigins (50 and 60 mg/kg) dose-dependently increased GE and SIT, whereas BTE 6% and thearubigins 70 mg/kg did not affect them. Sildenafil dose-dependently reduced both GE and SIT. Combination of metoclopramide, BTE 4.5%, thearubigins 60, or l-NAME with sildenafil (5 mg/kg) reversed its motility-delaying effects. Pretreatment with l-NAME followed by BTE 4.5%, thearubigins 60, BTE 4.5% + sildenafil 5, or thearubigins 60 + sildenafil 5 only partially affected the accelerating effects of BTE 4.5% and thearubigins 60. In conclusion, a standardized BTE and its thearubigins improve the sildenafil-induced delayed gut motility in mice. This improvement was partially blocked by l-NAME suggesting a possible role of nitric oxide. Thus, BTE 4.5% or TRs 60 mg/kg solution could be considered a reliever therapy for the sildenafil-induced dyspepsia.

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“…The PR method was used by multiple previous studies (Suchitra et al ., , de Rosalmeida et al . ; Firpo et al ., ; Qiu et al ., and Palheta et al ., ). It was validated by Bennink et al .…”

Section: Methodsmentioning

confidence: 99%

Black Tea Extract and its Thearubigins Relieve the Sildenafil‐Induced Delayed Gut Motility in Mice: A Possible Role of Nitric Oxide

Murad

Abdallah

2014

Phytotherapy Research

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“…The gastrointestinal tract transit assay was performed as described previously (Firpo et al, 2005) using WT 8-to 12-week-old male mice. Briefly, mice were fasted for 14 h with water available ad libitum, and then 5 mg/kg (200 l) of a test meal containing phenol red along with the TRPV2 agonists 10 M SNP or vehicle only was administered into the stomach using a feeding needle.…”

Section: Measurement Of No Release (Griess Assay)mentioning

confidence: 99%

Involvement of TRPV2 Activation in Intestinal Movement through Nitric Oxide Production in Mice

Mihara¹,

Boudaka²,

Shibasaki³

et al. 2010

J. Neurosci.

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Transient receptor potential channel vanilloid 2 (TRPV2) can detect various stimuli such as temperature (Ͼ52°C), stretch, and chemicals, including 2-aminoethoxydiphenyl borate, probenecid, and lysophospholipids. Although expressed in many tissues, including sensory and motor neurons, TRPV2 expression and function in the gastrointestinal tract is poorly understood. Here, we show TRPV2 expression in the murine intestine and its involvement in intestinal function. Almost all mouse intestinal intrinsic sensory and inhibitory motor neurons, both cell bodies and nerve fibers, showed TRPV2 immunoreactivity. Several known TRPV2 activators increased cytosolic Ca 2ϩ concentrations and evoked TRPV2-like current responses in dissociated myenteric neurons. Interestingly, mechanical stimuli activated inward currents in a strength-dependent manner, which were inhibited by a TRPV2 inhibitor tranilast. TRPV2 activation in isolated intestine inhibited spontaneous circular muscle contraction, which did not occur in the presence of the TRPV2 antagonist, tetrodotoxin or nitro oxide (NO) synthase pathway inhibitors. Also, increased intestinal NO production was observed in response to a TRPV2 agonist, and gastrointestinal transit in vivo was accelerated by TRPV2 agonists or an NO donor. In conclusion, TRPV2 may contribute to intestinal motility through NO production, and TRPV2 is a promising target for controlling intestinal movement.

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“…In mice, other methods for GI motility measures include evaluating the propulsion time of a bead inserted in the colon to a certain distance from anus and fecal pellet output in relation to the number and dry weight . Altered fecal output has been primarily associated with changes in colonic transit, but upper GI transit can also influence it . The OATT differences also reflect the fecal pellet elimination rate.…”

Section: Discussionmentioning

confidence: 77%

Gastrointestinal motility and morphology in mice: Strain‐dependent differences

Gama

Machado

Beckmann

et al. 2020

Neurogastroenterology Motil

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Background: BALB/c and C57BL/6 mice are widely used in biomedical research; however, the differences between strains are still underestimated. Our aims were to develop an experimental protocol to evaluate the duodenal contractility and gastrointestinal transit in mice using the Alternating Current Biosusceptometry (ACB) technique and to compare gastrointestinal motor function and morphology between BALB/c and C57BL/6 strains. Methods: Male mice were used in experiments (a) duodenal contractility: animals which had a magnetic marker surgically fixed in the duodenum to determine the frequency and amplitude of contractions and (b) gastrointestinal transit: animals which ingested a magnetically marked chow to calculate the Oro-Anal Transit Time (OATT) and the Fecal Pellet Elimination Rate (FPER). The animals were killed after the experiments for organ collection and morphometric analysis.Key Results: BALB/c and C57BL/6 had two different duodenal frequencies (high and low) with similar amplitudes. After 10 hours of monitoring, BALB/c eliminated around 89% of the ingested marker and C57BL/6 eliminated 33%; OATT and FPER were slower for C57BL/6 compared with BALB/c. The OATT and amplitude of low frequency had a strong positive correlation in C57BL/6. For BALB/c, the gastric muscular layer was thicker compared to that measured for C57BL/6. Conclusions and Inferences:The experimental protocol to evaluate duodenal contractility and fecal magnetic pellets output using the ACB technique in mice was successfully established. BALB/c strains had higher duodenal frequencies and a shorter time to eliminate the ingested marker. Our results showed differences in both motor function and gastrointestinal morphology between BALB/c and C57BL/6 strains. K E Y W O R D S BALB/c, C57BL/6, duodenal contractility, gastrointestinal transit, inbred S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section.

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A conscious mouse model of gastric ileus using clinically relevant endpoints

Cited by 12 publications

References 38 publications

Black Tea Extract and its Thearubigins Relieve the Sildenafil‐Induced Delayed Gut Motility in Mice: A Possible Role of Nitric Oxide

Black Tea Extract and its Thearubigins Relieve the Sildenafil‐Induced Delayed Gut Motility in Mice: A Possible Role of Nitric Oxide

Involvement of TRPV2 Activation in Intestinal Movement through Nitric Oxide Production in Mice

Gastrointestinal motility and morphology in mice: Strain‐dependent differences

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