A connection between MAPK pathways and circadian clocks

Paula, Renato M. de; Lamb, Teresa M.; Bennett, Lindsay; Bell-Pedersen, Deborah

doi:10.4161/cc.7.17.6516

Cited by 57 publications

(37 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, the ERK1/2 MAPK pathway is also involved in regulating circadian rhythms in SCN and plays an important role in the clock-resetting mechanisms of mammalian circadian clock (40,44,45). Interestingly, mTOR activity also exhibits circadian variations in SCN (46) and has been reported to be crucial for regulating protein synthesis by modulating the phosphorylation of 4EBP and mRNAs with 5Ј-TOP motifs (47,48).…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation of Eukaryotic Translation Initiation Factor 4E and Eukaryotic Translation Initiation Factor 4E-binding Protein (4EBP) and Their Upstream Signaling Components Undergo Diurnal Oscillation in the Mouse Hippocampus

Saraf

Luo

Morris

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: mRNA translation is crucial for formation of long-term memory. Results: Phosphorylation of eIF4E, 4EBPs, rpS6, Akt, mTOR, and ERK1/2 undergoes diurnal oscillation in mouse hippocampus. Conclusion: Diurnal oscillation in markers of mRNA translation initiation is critical for memory consolidation and persistence. Significance: Circadian oscillation in mRNA translation initiation provides a potential mechanism for persistence of long-term memory.

show abstract

Section: Discussionmentioning

confidence: 99%

Phosphorylation of Eukaryotic Translation Initiation Factor 4E and Eukaryotic Translation Initiation Factor 4E-binding Protein (4EBP) and Their Upstream Signaling Components Undergo Diurnal Oscillation in the Mouse Hippocampus

Saraf

Luo

Morris

et al. 2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Based on clock control of the OS MAPK pathway (15,23) and OS-2-dependent phosphorylation of RCK-2 ( Fig. 1), we predicted that the circadian clock regulates the accumulation of P-RCK-2 in constant environmental conditions.…”

Section: Significancementioning

confidence: 99%

Circadian clock regulation of mRNA translation through eukaryotic elongation factor eEF-2

Caster

Castillo

Sachs

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

The circadian clock has a profound effect on gene regulation, controlling rhythmic transcript accumulation for up to half of expressed genes in eukaryotes. Evidence also exists for clock control of mRNA translation, but the extent and mechanisms for this regulation are not known. In Neurospora crassa, the circadian clock generates daily rhythms in the activation of conserved mitogen-activated protein kinase (MAPK) pathways when cells are grown in constant conditions, including rhythmic activation of the well-characterized p38 osmosensing (OS) MAPK pathway. Rhythmic phosphorylation of the MAPK OS-2 (P-OS-2) leads to temporal control of downstream targets of OS-2. We show that osmotic stress in N. crassa induced the phosphorylation of a eukaryotic elongation factor-2 (eEF-2) kinase, radiation sensitivity complementing kinase-2 (RCK-2), and that RCK-2 is necessary for high-level phosphorylation of eEF-2, a key regulator of translation elongation. The levels of phosphorylated RCK-2 and phosphorylated eEF-2 cycle in abundance in wild-type cells but not in cells deleted for OS-2 or the core clock component FREQUENCY (FRQ). Translation extracts from cells grown in constant conditions show decreased translational activity in the late subjective morning, coincident with the peak in eEF-2 phosphorylation, and rhythmic translation of glutathione S-transferase (GST-3) from constitutive mRNA levels in vivo is dependent on circadian regulation of eEF-2 activity. In contrast, rhythms in phosphorylated eEF-2 levels are not necessary for rhythms in accumulation of the clock protein FRQ, indicating that clock control of eEF-2 activity promotes rhythmic translation of specific mRNAs.C ircadian rhythms are the outward manifestation of an endogenous clock mechanism common to nearly all eukaryotes. Depending on the organism and tissue, nearly half of an organism's expressed genes are under control of the circadian clock at the level of transcription (1-4). However, mounting evidence indicates a role for the clock in controlling posttranscriptional mechanisms (5), including translation initiation (6, 7), whereas clock control of translation elongation has not been investigated.The driver of circadian rhythms in Neurospora crassa is an autoregulatory molecular feedback loop composed of the negative elements FREQUENCY (FRQ) and FRQ RNA-interacting helicase (FRH), which inhibit the activity of the positive elements WHITE COLLAR-1 (WC-1) and WC-2 (8-11). WC-1 and WC-2 heterodimerize to form the white collar complex (WCC), which activates transcription of frequency (frq) (8,12,13) and activates transcription of a large set of downstream target genes important for overt rhythmicity (2, 14). One gene directly controlled by the WCC is os-4, which encodes the mitogen-activated protein kinase kinase kinase (MAPKKK) in the osmotically sensitive (OS) MAPK pathway (15). Rhythmic transcription of os-4 leads to rhythmic accumulation of the phosphorylated active form of the downstream p38-like mitogen-activated protein kinase (MAPK) OS-2 (16). In Sac...

show abstract

“…The list includes the nine components of the three MAPK cascades (24,42,49,53,56,68), PKA (5,32), PKC (23), checkpoint kinase 2 (73), ATM (Ataxia telangiectasia mutated; mus-21) (87), ATR (ATM and Rad3-related) homologs (mus-9) (87), the NDR (nuclear Dbf2-related) family of S/T kinases, cot-1 and dbf-2 (20,95), and others (see Table 1 for a complete list). Characterized Neurospora kinases have been shown to regulate a diversity of cellular functions, including polarized growth, hyphal fusion, asexual sporulation (conidiation), female sexual development, stress regulation, DNA damage responses, and the circadian clock (4,18,20,22,60,68,73,84,87,95).…”

mentioning

confidence: 99%

Global Analysis of Serine-Threonine Protein Kinase Genes in Neurospora crassa

Park

Servin

Turner³

et al. 2011

Eukaryot Cell

110

View full text Add to dashboard Cite

Serine/threonine (S/T) protein kinases are crucial components of diverse signaling pathways in eukaryotes, including the model filamentous fungus Neurospora crassa. In order to assess the importance of S/T kinases to Neurospora biology, we embarked on a global analysis of 86 S/T kinase genes in Neurospora. We were able to isolate viable mutants for 77 of the 86 kinase genes. Of these, 57% exhibited at least one growth or developmental phenotype, with a relatively large fraction (40%) possessing a defect in more than one trait. S/T kinase knockouts were subjected to chemical screening using a panel of eight chemical treatments, with 25 mutants exhibiting sensitivity or resistance to at least one chemical. This brought the total percentage of S/T mutants with phenotypes in our study to 71%. Mutants lacking apg-1, an S/T kinase required for autophagy in other organisms, possessed the greatest number of phenotypes, with defects in asexual and sexual growth and development and in altered sensitivity to five chemical treatments. We showed that NCU02245/stk-19 is required for chemotropic interactions between female and male cells during mating. Finally, we demonstrated allelism between the S/T kinase gene NCU00406 and velvet (vel), encoding a p21-activated protein kinase (PAK) gene important for asexual and sexual growth and development in Neurospora.

show abstract

A connection between MAPK pathways and circadian clocks

Cited by 57 publications

References 67 publications

Phosphorylation of Eukaryotic Translation Initiation Factor 4E and Eukaryotic Translation Initiation Factor 4E-binding Protein (4EBP) and Their Upstream Signaling Components Undergo Diurnal Oscillation in the Mouse Hippocampus

Phosphorylation of Eukaryotic Translation Initiation Factor 4E and Eukaryotic Translation Initiation Factor 4E-binding Protein (4EBP) and Their Upstream Signaling Components Undergo Diurnal Oscillation in the Mouse Hippocampus

Circadian clock regulation of mRNA translation through eukaryotic elongation factor eEF-2

Global Analysis of Serine-Threonine Protein Kinase Genes in Neurospora crassa

Contact Info

Product

Resources

About