A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis.

Wu, Ling Juan; Lewis, Peter J.; Allmansberger, Rudolf; Hauser, Philippe M.; Errington, Jeff

doi:10.1101/gad.9.11.1316

Cited by 162 publications

(194 citation statements)

References 51 publications

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“…The following E. coli K12 derivatives were used: YK1100 (trpC994) (Yamanaka et al 1996), PC2 (dnaC2 thy leu rspL) (Carl 1970), and CM748 (dnaA203 metE46 trp-3 his-4 thi-1 galK2 lacY1 or lacZ4 mtl-1 ara-9 tsx-3 ton-1 rps-8 or rps-9 supE44) (Hansen et al 1984). L medium (Hiraga et al 1989) was used for growth of YK1100 and CM748.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Bidirectional migration of SeqA‐bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli

et al. 2000

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Section: Bacterial Strainsmentioning

confidence: 99%

Bidirectional migration of SeqA‐bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli

et al. 2000

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“…Moreover, SpoIIIE affects the topology of plasmid molecules, consistent with tracking of the protein along the DNA (Bath et al 2000). It has been determined that the DNA located in the prespore of a spoIIIE mutant always consists of the same region of chromosome, corresponding to a 500 kb region surrounding the origin of replication, oriC (Wu & Errington 1994;Wu et al 1995). SpoIIIE homologues are widespread in non-sporulating bacteria, including the conserved cell division protein FtsK.…”

Section: Orientation Positioning and Movement Of The Bacterial Chrommentioning

confidence: 99%

Towards understanding the molecular basis of bacterial DNA segregation

Leonard

Møller‐Jensen

Löwe

2005

Phil. Trans. R. Soc. B

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Bacteria ensure the fidelity of genetic inheritance by the coordinated control of chromosome segregation and cell division. Here, we review the molecules and mechanisms that govern the correct subcellular positioning and rapid separation of newly replicated chromosomes and plasmids towards the cell poles and, significantly, the emergence of mitotic-like machineries capable of segregating plasmid DNA. We further describe surprising similarities between proteins involved in DNA partitioning (ParA/ParB) and control of cell division (MinD/MinE), suggesting a mechanism for intracellular positioning common to the two processes. Finally, we discuss the role that the bacterial cytoskeleton plays in DNA partitioning and the missing link between prokaryotes and eukaryotes that is bacterial mechano-chemical motor proteins.

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“…S8A). Moreover, in a SpoIIIE point mutant (SpoIIIE36) that binds DNA but is impaired in DNA transport (Wu and Errington 1994;Wu et al 1995), FisB protein levels were unaffected, but YFP-FisB localization was disrupted (Supplemental Fig. S8B).…”

Section: Spoiiie and Membrane Fissionmentioning

confidence: 99%

FisB mediates membrane fission during sporulation in Bacillus subtilis

et al. 2013

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How bacteria catalyze membrane fission during growth and differentiation is an outstanding question in prokaryotic cell biology. Here, we describe a protein (FisB, for fission protein B) that mediates membrane fission during the morphological process of spore formation in Bacillus subtilis. Sporulating cells divide asymmetrically, generating a large mother cell and smaller forespore. After division, the mother cell membranes migrate around the forespore in a phagocytic-like process called engulfment. Membrane fission releases the forespore into the mother cell cytoplasm. Cells lacking FisB are severely and specifically impaired in the fission reaction. Moreover, GFP-FisB forms dynamic foci that become immobilized at the site of fission. Purified FisB catalyzes lipid mixing in vitro and is only required in one of the fusing membranes, suggesting that FisB-lipid interactions drive membrane remodeling. Consistent with this idea, the extracytoplasmic domain of FisB binds with remarkable specificity to cardiolipin, a lipid enriched in the engulfing membranes and regions of negative curvature. We propose that membrane topology at the final stage of engulfment and FisB-cardiolipin interactions ensure that the mother cell membranes are severed at the right time and place. The unique properties of FisB set it apart from the known fission machineries in eukaryotes, suggesting that it represents a new class of fission proteins.

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A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis.

Abstract: Spore formation inthrough the newly formed spore septum. We propose that translocation of the prespore chromosome occurs by a mechanism that is functionally related to the conjugative transfer of plasmid DNA.

Cited by 162 publications

References 51 publications

Bidirectional migration of SeqA‐bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli

Bidirectional migration of SeqA‐bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli

Towards understanding the molecular basis of bacterial DNA segregation

FisB mediates membrane fission during sporulation in Bacillus subtilis

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