A confocal laser scanning and conventional wide field light microscopy study ofClassopollisfrom the Toarcian‐Aalenian of the Fuentelsaz section (Spain)

Abstract

A morphological study of three different types of dispersed Circumpolles pollen grains from the Toarcian-Aalenian of the Fuentelsaz section (Spain) was performed using conventional wide field and confocal laser scanning microscopy (CSLM). The CSLM data allowed scanning electron microscope-like three-dimensional reconstructions to be made and showed that the most part of the studied pollen have taxonomic features characteristic of the Mesozoic genus Classopollis. At the species level, Classopollis torosus and Classopollis classoides were identified. The third form studied is characterized by the presence of Ubisch bodies adhering to the pollen wall.

Cheirolepidiacean foliage and pollen from Cretaceous high-latitudes of southeastern Australia

“…However, as discussed by Peyrot et al (2007), the loss of the type material precludes any comparison. C. classoides was described by Pocock and Jansonius (1961) as having a granulate sculpture whereas C. torosus was described by Couper (1958) and Burger (1965) as having a scabrate exine, as was C. sp.…”

“…Pocock and Jansonius (1961) described continuous bands of sculpture around the equator of C. classoides whilst Couper (1958) and Burger (1965) suggested that C. torosus has forms that have continuous endostriae or features that are not continuous but are formed by discrete granules. Peyrot et al (2007) attempted to distinguish the two species based on SEM, TEM and CSLM and determined that their Type B grains were related to C. torosus, based on a thinner scabrate exine than their Type A, which had features that resembled the description of C. classoides by Pocock and Jansonius (1961). This is in agreement with the measurements by Reyre (1970) who indicated that C. classoides has a thicker exine (1-2 μm) than C. torosus (0.75-1.0 μm).…”

Preliminary Revision of the Palynological Collection of Professor Blanka Pacltová – A Significant Collection of Cenomanian Microflora Housed at the National Museum, Prague

“…indet. ; see Peyrot et al 2007) with the aid of both light microscope and confocal laser scanning did not allow description of a new species, nor comparison with others. At least, it would be necessary to use a SEM as e.g.…”

Application of confocal laser-scanning microscopy (CLSM) to autofluorescent organic and mineral matter in peat, coals and siliciclastic sedimentary rocks — A qualitative approach

“…These standard high resolution techniques are, however, limited to the surface plane ultrastructure and morphology of the investigated specimens. With the application of CLSM, examination of both 2D surface and deep structure as well as 3D reconstructions of autofluorescent fossil pollen grains in organic specimens obtained from cleaned and digested sedimentary rocks became possible (Hochuli and FeistBurkhardt, 2004;Peyrot, et al, 2007;Vitha et al, 2009). Investigation of pollen ultrastructure and detailed morphology requires application of thorough sample preparation procedures including crushing, cleaning, fracturing, drying as well as specific mounting methods (Collinson, 1999;Jones and Rowe, 1999).…”