A concise tutorial review of TOF-SIMS based molecular and cellular imaging

Massonnet, Philippe; Heeren, Ron M. A.

doi:10.1039/c9ja00164f

Cited by 62 publications

(47 citation statements)

References 144 publications

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“…large Ar clusters. 270 High ion doses allow depth proling. 270 NanoSIMS studies of 300 nm-thick sections of resinembedded colon cancer cells treated with 15 N-labelled cisplatin (10-150 mM) suggest partial dissociation of Pt-N bonds in at least the nucleoli, based on the Pt/N stoichiometry, and co-localisation of Pt with S and P (detection of 194 Pt, 34 S and 15 N signals), consistent with the affinity of Pt II for S-rich proteins and DNA.…”

Section: Imaging Mass Spectrometrymentioning

confidence: 99%

“…270 High ion doses allow depth proling. 270 NanoSIMS studies of 300 nm-thick sections of resinembedded colon cancer cells treated with 15 N-labelled cisplatin (10-150 mM) suggest partial dissociation of Pt-N bonds in at least the nucleoli, based on the Pt/N stoichiometry, and co-localisation of Pt with S and P (detection of 194 Pt, 34 S and 15 N signals), consistent with the affinity of Pt II for S-rich proteins and DNA. 271 Similarly, correlative nanoSIMS and electron microscopy imaging of Au III mesoporphyrin IX dimethyl ester (Au2) in A2780 cells suggests that this anticancer complex attacks sulfurrich proteins in mitochondria ( 197 Au in regions with high 32 S/ 12 C 14 N ratios).…”

Section: Imaging Mass Spectrometrymentioning

confidence: 99%

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Metallodrugs are unique: opportunities and challenges of discovery and development

et al. 2020

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Section: Imaging Mass Spectrometrymentioning

confidence: 99%

Section: Imaging Mass Spectrometrymentioning

confidence: 99%

Metallodrugs are unique: opportunities and challenges of discovery and development

et al. 2020

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“…In both LESA and 3D OrbiSIMS, ions are generated in a complementary manner. Whilst nanoESI predominantly generates adduct ions of the parent molecule, SIMS spectra can contain numerous peaks which can be either intact molecules or fragments thereof (35). This makes the automated assignment of SIMS secondary ions to metabolite identities a challenge.…”

Section: Resultsmentioning

confidence: 99%

Sequential 3D OrbiSIMS and LESA-MS/MS-based metabolomics for prediction of brain tumor relapse from sample-limited primary tissue archives

Meurs¹,

Scurr²,

Lourdusamy³

et al. 2020

Preprint

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Surgically-resected biopsies from childhood brain tumors are archived in a formalin-fixed paraffin-embedded (FFPE) state. Identified key tumor regions are conserved through the fabrication of tissue microarrays (TMA). The vast amount of TMA libraries available have the potential to be used for metabolite profiling; however, due to incompatibility of TMAs with current analysis strategies, the potential has been unexplored. The recent emergence of liquid extraction surface analysis tandem mass spectrometry (LESA-MS/MS) and Orbitrap secondary ion mass spectrometry (3D OrbiSIMS) allows for a novel untargeted metabolite profiling and imaging approach for characterization of tumor TMAs. This strategy employs serial mass spectrometry (MS) analysis, with the high mass resolving imaging power of 3D OrbiSIMS combining complementarity, with the detection of a greater number of metabolites using LESA-MS/MS. Using multivariate analysis on data generated from both techniques permits the identification of metabolites that group patients based on eventual tumor recurrence, leading to the discovery of novel affected metabolic pathways and the prediction of tumor recurrence with high confidence. Moreover, both techniques reveal a unique set of classifiers and metabolic pathways for predicting pediatric ependymoma relapse from a set of primary tumors. The serial MS strategy opens new opportunities to retrospectively link molecular data from any FFPE TMA library with clinical outcomes and to discover novel drug targets with the aim of developing stratified drug treatments.

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“…The resulting composition, corresponding to different depths of the sputtering process and planar position of the rastering primary beam, is used to reconstruct the 3D cross section of the specimen, complementing the results from STEM and EDX spectroscopy. However, appropriate calibration standards are required for quantitative depth-profiling [ 68 ]. ToF-SIMS has been used to understand the depth distribution of oxides after SIS treatment, usually adopted in homopolymer layers such as PMMA [ 56 , 63 ] and PS [ 56 ], PET film and fibers [ 54 ], but also in block copolymers layers such as PS- b -P2VP both as micellar films [ 58 ] and self-assembled nanodomains infiltrated with

[ 43 ].…”

Section: Characterization Techniquesmentioning

confidence: 99%

Recent Advances in Sequential Infiltration Synthesis (SIS) of Block Copolymers (BCPs)

et al. 2021

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In the continuous downscaling of device features, the microelectronics industry is facing the intrinsic limits of conventional lithographic techniques. The development of new synthetic approaches for large-scale nanopatterned materials with enhanced performances is therefore required in the pursuit of the fabrication of next-generation devices. Self-assembled materials as block copolymers (BCPs) provide great control on the definition of nanopatterns, promising to be ideal candidates as templates for the selective incorporation of a variety of inorganic materials when combined with sequential infiltration synthesis (SIS). In this review, we report the latest advances in nanostructured inorganic materials synthesized by infiltration of self-assembled BCPs. We report a comprehensive description of the chemical and physical characterization techniques used for in situ studies of the process mechanism and ex situ measurements of the resulting properties of infiltrated polymers. Finally, emerging optical and electrical properties of such materials are discussed.

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A concise tutorial review of TOF-SIMS based molecular and cellular imaging

Abstract: This concise tutorial review provides a description of the current state of the art in the application of time-of flight based secondary ion mass spectrometry (TOF-SIMS) in the field of molecular and cellular imaging.

Cited by 62 publications

References 144 publications

Metallodrugs are unique: opportunities and challenges of discovery and development

Metallodrugs are unique: opportunities and challenges of discovery and development

Sequential 3D OrbiSIMS and LESA-MS/MS-based metabolomics for prediction of brain tumor relapse from sample-limited primary tissue archives

Recent Advances in Sequential Infiltration Synthesis (SIS) of Block Copolymers (BCPs)

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