A concise review of common animal models for the study of limb regeneration

Farah, Zayd; Fan, Huimin; Liu, Zhongmin; He, Jia‐Qiang

doi:10.1080/15476278.2016.1205775

Cited by 9 publications

(6 citation statements)

References 58 publications

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“…Although the efficiency of hard tissue regeneration differs in model animals, regeneration studies in large mammals (e.g., dogs) have been reported to be more challenging than in small animals (e.g., mice) [ 39 , 40 ]. Nevertheless, large mammals provide a more appropriate model for mimicking human disease and are thus more transferable to a human model.…”

Section: Discussionmentioning

confidence: 99%

Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide

et al. 2020

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We aim to examine the effects of a newly developed peptide derived from CPNE7 (Cpne7-DP) in tertiary dentin formation and peritubular space occlusion, and comprehensively evaluate its potential as a bioactive therapeutic agent. Human dental pulp cells (HDPCs) and a mouse pre-odontoblast cell line, MDPC-23, were chosen for in vitro studies to characterize lineage-specific cell responses after Cpne7-DP treatment. Whether Cpne7-DP reproduces the dentin regenerative potential of CPNE7 was tested using a beagle dog model by generating dentinal defects of various degrees in vivo. Peritubular space occlusion was further examined by scanning electron microscopy and microleakage test, while overall mineralization capacity of Cpne7-DP was tested ex vivo. CPNE7 promotes tubular dentin formation under both shallow and deep dentinal defects, and the functional peptide Cpne7-DP induces odontoblast-like differentiation in vitro, mineralization ex vivo, and tubular dentin formation in in vivo beagle dog dentin exposure and pulp exposure models. Moreover, Cpne7-DP leads to peritubular space occlusion and maintains stability under different conditions. We show that CPNE7 and its derivative functional peptide Cpne7-DP promotes dentin regeneration in dentinal defects of various degrees and that the regenerated hard tissue demonstrates the characteristics of true dentin. Limitations of the current dental materials including post-operative hypersensitivity make biological repair of dentin a field of growing interest. Here, we suggest that the dual functions of Cpne7-DP in tubular dentin formation and peritubular space occlusion are promising for the treatment of dentinal loss and sensitivity.

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Section: Discussionmentioning

confidence: 99%

Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide

et al. 2020

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“…Several animal models for the study of limb regeneration have been described, among them the Mexican axolotl (Ambystoma mexicanum) [ 89 , 90 , 91 , 92 ]. It turned out that.…”

Section: A Key Role Of Stem Cell Therapy In Re-establishing Tissue Homeostasis Between Dying and Replacing Cellsmentioning

confidence: 99%

Perspective: Why and How Ubiquitously Distributed, Vascular-Associated, Pluripotent Stem Cells in the Adult Body (vaPS Cells) Are the Next Generation of Medicine

Alt

Schmitz

Bai

2021

Cells

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A certain cell type can be isolated from different organs in the adult body that can differentiate into ectoderm, mesoderm, and endoderm, providing significant support for the existence of a certain type of small, vascular-associated, pluripotent stem cell ubiquitously distributed in all organs in the adult body (vaPS cells). These vaPS cells fundamentally differ from embryonic stem cells and induced pluripotent stem cells in that the latter possess the necessary genetic guidance that makes them intrinsically pluripotent. In contrast, vaPS cells do not have this intrinsic genetic guidance, but are able to differentiate into somatic cells of all three lineages under guidance of the microenvironment they are located in, independent from the original tissue or organ where they had resided. These vaPS cells are of high relevance for clinical application because they are contained in unmodified, autologous, adipose-derived regenerative cells (UA-ADRCs). The latter can be obtained from and re-applied to the same patient at the point of care, without the need for further processing, manipulation, and culturing. These findings as well as various clinical examples presented in this paper demonstrate the potential of UA-ADRCs for enabling an entirely new generation of medicine for the benefit of patients and healthcare systems.

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“…To our knowledge, such model organisms are not known. Several animal models for the study of limb regeneration have been described, among them the Mexican axolotl (Ambystoma mexicanum) (c.f., e.g., McCusker and Gardiner, 2011;Farah et al, 2016;Grillo, Konstantinides and Averof, 2016). It turned out that in limb regeneration, a morphologically uniform intermediate (the so-called blastema) is formed, consisting of a variety of multipotent stem and progenitor cells originating from a variety of tissues (Zielins et al, 2016).…”

Section: A Key Role Of Stem Cell Therapy In Reestablishing Tissue Hommentioning

confidence: 99%

Fundamentals of Stem Cells: Why and How Patients' Own Adult Stem Cells Are the Next Generation of Medicine

Alt¹,

Schmitz²,

Bai³

2019

Preprint

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Various tissue resident stem cells are receiving attention from basic scientists and clinicians as they hold certain promise for regenerative medicine. This paper is intended to clarify and facilitate the understanding, development and adoption of regenerative medicine in general and specifically of therapies based on unmodified, autologous adipose-derived regenerative cells (UA-ADRCs). To this end, results of landmark experiments on stem cells and stem cell therapy performed in the labs of the authors are summarized, the most intriguing of which are the following: (i) vascular associated mesenchymal stem cells (MSCs) can be isolated from different organs (adipose tissue, heart, skin, bone marrow and skeletal muscle) and differentiated into ectoderm, mesoderm and endoderm, providing significant support for the hypothesis of the existence of a small, ubiquitously distributed, universal vascular associated stem cell with full pluripotency; (ii) the orientation and differentiation of MSCs are driven by signals of the respective microenvironment; and (iii) these stem cells irrespective of the tissue origin exhibit full pluripotent differentiation potential without any prior genetic modification or the need for culturing. They can be obtained from a small amount of adipose tissue when using the appropriate technology for isolating the cells, and can be harvested from and re-applied to the same patient at the point of care without the need for complicated processing, manipulation, culturing, expensive equipment, or repeat interventions. These findings demonstrate the potential of UA-ADRCs for triggering the development of an entire new generation of medicine for the benefit of patients and of healthcare systems.

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A concise review of common animal models for the study of limb regeneration

Cited by 9 publications

References 58 publications

Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide

Tubular Dentin Regeneration Using a CPNE7-Derived Functional Peptide

Perspective: Why and How Ubiquitously Distributed, Vascular-Associated, Pluripotent Stem Cells in the Adult Body (vaPS Cells) Are the Next Generation of Medicine

Fundamentals of Stem Cells: Why and How Patients' Own Adult Stem Cells Are the Next Generation of Medicine

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