A Concerted Action of Engrailed and Gooseberry-Neuro in Neuroblast 6-4 Is Triggering the Formation of Embryonic Posterior Commissure Bundles

Colomb, S.; Joly, Willy; Bonneaud, Nathalie; Maschat, Florence

doi:10.1371/journal.pone.0002197

Cited by 11 publications

(8 citation statements)

References 62 publications

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“…Both cells express Gooseberry proximal (Gsb-p; now known as Gsb-n -FlyBase; Fig. 4E), which is activated by gsb-d (Buenzow and Holmgren, 1995) and thus labels NBs that once expressed gsb-d (Colomb et al, 2008). Both cells are En negative (Fig.…”

Section: Research Articlementioning

confidence: 99%

Neuroblast pattern and identity in the Drosophila tail region and role of doublesex in the survival of sex-specific precursors

et al. 2013

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SUMMARYThe central nervous system is composed of segmental units (neuromeres), the size and complexity of which evolved in correspondence to their functional requirements. In Drosophila, neuromeres develop from populations of neural stem cells (neuroblasts) that delaminate from the early embryonic neuroectoderm in a stereotyped spatial and temporal pattern. Pattern units closely resemble the ground state and are rather invariant in thoracic (T1-T3) and anterior abdominal (A1-A7) segments of the embryonic ventral nerve cord. Here, we provide a comprehensive neuroblast map of the terminal abdominal neuromeres A8-A10, which exhibit a progressively derived character. Compared with thoracic and anterior abdominal segments, neuroblast numbers are reduced by 28% in A9 and 66% in A10 and are almost entirely absent in the posterior compartments of these segments. However, all neuroblasts formed exhibit serial homology to their counterparts in more anterior segments and are individually identifiable based on their combinatorial code of marker gene expression, position, delamination time point and the presence of characteristic progeny cells. Furthermore, we traced the embryonic origin and characterised the postembryonic lineages of a set of terminal neuroblasts, which have been previously reported to exhibit sex-specific proliferation behaviour during postembryonic development. We show that the respective sex-specific product of the gene doublesex promotes programmed cell death of these neuroblasts in females, and is needed for their survival, but not proliferation, in males. These data establish the terminal neuromeres as a model for further investigations into the mechanisms controlling segment-and sex-specific patterning in the central nervous system.

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Section: Research Articlementioning

confidence: 99%

Neuroblast pattern and identity in the Drosophila tail region and role of doublesex in the survival of sex-specific precursors

et al. 2013

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“…In Drosophila, En is an important transcription factor in anteriorposterior (A/P) wing patterning (Morata and Lawrence, 1975;Lawrence and Morata, 1976) and in neurodevelopment (Bhat and Schedl, 1997;Colomb et al, 2008). The larval wing imaginal disc, which gives rise to the adult wing, is subdivided into nonintermingling anterior (A) and posterior (P) compartments.…”

Section: Introductionmentioning

confidence: 99%

Engrailed homeoprotein acts as a signaling molecule in the developing fly

et al. 2011

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SUMMARYHomeodomain transcription factors classically exert their morphogenetic activities through the cell-autonomous regulation of developmental programs. In vertebrates, several homeoproteins have also been shown to have direct non-cell-autonomous activities in the developing nervous system. We present the first in vivo evidence for homeoprotein signaling in Drosophila. Focusing on wing development as a model, we first demonstrate that the homeoprotein Engrailed (En) is secreted. Using singlechain anti-En antibodies expressed under the control of a variety of promoters, we delineate the wing territories in which secreted En acts. We show that En is a short-range signaling molecule that participates in anterior crossvein development, interacting with the Dpp signaling pathway. This report thus suggests that direct signaling with homeoproteins is an evolutionarily conserved phenomenon that is not restricted to neural tissues and involves interactions with bona fide signal transduction pathways.

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“…Mutant strains were obtained from the Bloomington Drosophila Stock Center. Gal4 and UAS lines employed were: sim-Gal4 (Xiao et al, 1996), prd-Gal4 (Xiao et al, 1996), UAS-ci.VP16 (Larsen et al, 2003), UAS-en (Guillén et al, 1995), UAS-gsb (Marie et al, 2010), UAS-gsb-n (Colomb et al, 2008), UAS-hh (Porter et al, 1996), UAS-slp1 (Sato and Tomlinson, 2007) and UAS-tau-GFP (Brand, 1995). The ptc 7 wg [1][2][3][4][5][6][7][8][9][10][11][12] strain was grown at 29°C to reduce wg function.…”

Section: Drosophila Strains and Genetic Analysismentioning

confidence: 99%

Formation and specification of a Drosophila dopaminergic precursor cell

Watson

Crews

2012

Development

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Dopaminergic neurons play important roles in animal behavior, including motivation, reward and locomotion. The Drosophila dopaminergic H-cell interneuron is an attractive system for studying the genetics of neural development because analysis is focused on a single neuronal cell type. Here we provide a mechanistic understanding of how MP3, the precursor to the H-cell, forms and acquires its identity. We show that the gooseberry/gooseberry-neuro (gsb/gsb-n) transcription factor genes act to specify MP3 cell fate. It is proposed that single-minded commits neuroectodermal cells to a midline fate, followed by a series of signaling events that result in the formation of a single gsb+/gsb-n+ MP3 cell per segment. The wingless signaling pathway establishes a midline anterior domain by activating expression of the forkhead transcription factors sloppy paired 1 and sloppy paired 2. This is followed by hedgehog signaling that activates gsb/gsb-n expression in a subgroup of anterior cells. Finally, Notch signaling results in the selection of a single MP3, with the remaining cells becoming midline glia. In MP3, gsb/gsb-n direct H-cell development, in large part by activating expression of the lethal of scute and tailup H-cell regulatory genes. Thus, a series of signaling and transcriptional events result in the specification of a unique dopaminergic precursor cell. Additional genetic experiments indicate that the molecular mechanisms that govern MP3/H-cell development might also direct the development of non-midline dopaminergic neurons.

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A Concerted Action of Engrailed and Gooseberry-Neuro in Neuroblast 6-4 Is Triggering the Formation of Embryonic Posterior Commissure Bundles

Cited by 11 publications

References 62 publications

Neuroblast pattern and identity in the Drosophila tail region and role of doublesex in the survival of sex-specific precursors

Neuroblast pattern and identity in the Drosophila tail region and role of doublesex in the survival of sex-specific precursors

Engrailed homeoprotein acts as a signaling molecule in the developing fly

Formation and specification of a Drosophila dopaminergic precursor cell

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