A Conceptual Outline for Omics Experiments Using Bioinformatics Analogies

Suravajhala, Prashanth; Bizzaro, Jeffrey W.

doi:10.21769/bioprotoc.1387

Cited by 1 publication

(1 citation statement)

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“…We have reviewed such tools used for functional annotation of HPs in Table 1 . Recently, a conceptualized outline for ‘Omics Experiments Using Bioinformatics Analogies’ have been designated to represent HPs as an example ( Suravajhala and Bizzaro, 2015 ). The predictions need to be authenticated or validated by in vitro and/or in vivo experiments to further characterize the predicted functionality.…”

Section: Annotation Linked To Structural and Functional Predictionmentioning

confidence: 99%

Annotation and curation of uncharacterized proteins- challenges

Ijaq¹,

Chandrasekharan²,

Poddar³

et al. 2015

Front. Genet.

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Hypothetical proteins (HPs) are the proteins predicted to be expressed from an open reading frame, making a substantial fraction of proteomes in both prokaryotes and eukaryotes. Genome projects have led to the identification of many therapeutic targets, the putative function of the protein, and their interactions. In this review we enlist various methods linking annotation to structural and functional prediction of HPs that assist in the discovery of new structures and functions serving as markers and pharmacological targets for drug designing, discovery, and screening. Further we give an overview of how mass spectrometry as an analytical technique is used to validate protein characterisation. We discuss how microarrays and protein expression profiles help understanding the biological systems through a systems-wide study of proteins and their interactions with other proteins and non-proteinaceous molecules to control complex processes in cells. Finally, we articulate challenges on how next generation sequencing methods have accelerated multiple areas of genomics with special focus on uncharacterized proteins.

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Section: Annotation Linked To Structural and Functional Predictionmentioning

confidence: 99%