A concept development of an early warning system for toxic sewage detection

Jurga, Anna; Gemza, Natalia; Janiak, Kamil

doi:10.1051/e3sconf/20171700036

Cited by 2 publications

(4 citation statements)

References 8 publications

(17 reference statements)

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“…Large percentage (> 94%) of the cefotaxime-resistant E. coli exhibited multiple antibiotic indices (MAR Index) > 0.2 which means that the isolates might have originated from high risk source (s), where antibiotics are probably abused [38,40,59]. As noted earlier, since the WWTPs are known as early warning systems [47], the MAR Indices in this study revealed that antibiotics are in constant abuse and hence, their high selective pressure [61]. The high resistance to colistin sulphate (76.5%) detected in this study is considered alarming.…”

Section: Discussionmentioning

confidence: 94%

“…The presence of the latter (cefotaxime-resistant E. coli) might also be due to wastewater from hospital inflow. These resistance attributes are expected from the clinical origin [20,21,46] and since the WWTPs are known as early warning system [47], the presence of a large number of cefotaximeresistant E. coli might be a warning sign of probable lack of pretreatment for the hospital outflow. The effluent concentration of both total E. coli and cefotaxime-resistant E. coli (range in log 10 CFU/mL = 0 to 0.3) (Fig.…”

Section: Discussionmentioning

confidence: 99%

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Antibiogram and beta-lactamase genes among cefotaxime resistant E. coli from wastewater treatment plant

Adegoke

Madu

Aiyegoro

et al. 2020

Antimicrob Resist Infect Control

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Background: The World Health Organization (WHO) recently classified Enterobacteriaceae resistance to thirdgeneration cephalosporin into the group of pathogens with critical criteria for future research. Methods: A study to assess the antibiogram and beta-lactamase genes among the cefotaxime resistant E. coli (CREc) from a South African wastewater treatment plant (WWTP) was conducted using standard phenotypic and molecular biology characterization methods. Results: Approximate total E. coli (TEc) concentration (log 10 CFU/mL) ranged between 5.7 and 6.8 among which cefotaxime resistant E. coli were between 1.8 and 4.8 (log 10 CFU/mL) for cefotaxime antibiotic concentration of 4 and 8 mg/L in the influent samples. Effluent samples, heavily influenced by the chlorination had only 0.3 log 10 CFU/ mL of TEc. Fifty-one cefotaxime resistant isolates were selected out of an overall of 75 isolates, and subjected to a new round of testing, with a follow up of 36 and 48 isolates for both colistin and gentamicin, respectively as guided by initial results. Selected CREc exhibited resistance to amoxicillin-clavulanic acid (35.3%; n = 51), colistin sulphate (76.5%; n = 36), ciprofloxacin (47.1%; n = 51), gentamicin (87.5%; n = 48) and intermediate-resistance to meropenem (11.8%; n = 51). Extended spectrum-beta-lactamase genes detected, viz.: bla CTX-M (52.6%; n = 38) and bla TEM (84.2%; n = 38) and concurrent bla CTX-M + bla TEM (36.8%; n = 38), but no bla SHV was detected. Carbapenem resistance genes, blaKPC-2 (15.8%; n = 38), blaOXA-1 (57.9%; n = 38), blaNDM-1 (15.8%; n = 38) were also detected. Approximately, 10.5-36.8% (n = 38) co-occurrence of two or more beta-lactamase genes was detected in some isolates. Out of the selected number (n = 30), 7(23.3%) were enterotoxigenic E. coli (ETEC), 14 (46.7%) were Enteroaggregative E. coli (EAEC), but no enteropathogenic E. coli (EPEC) was detected. Conclusion: Resistance to cefotaxime and the presence of a wide range of beta-lactamase genes exposed the potential risks associated with these pathogens via occupational and domestic exposure during the reuse of treated wastewater.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Antibiogram and beta-lactamase genes among cefotaxime resistant E. coli from wastewater treatment plant

Adegoke

Madu

Aiyegoro

et al. 2020

Antimicrob Resist Infect Control

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“…In extreme cases the bacteria are killed by toxic wastewater, and there is a need for cleanout and reseeding of plant, which is a costly and time-consuming operation [2]. Toxic inflow can cause collapse of nitrification process and lead to significantly exceeded total nitrogen concentration in effluent [4]. Wastewater streams from drug manufacturing also contain residues of active pharmaceutical ingredients (API) and their intermediates, which are classified as micropollutant of emerging concern.…”

Section: Introductionmentioning

confidence: 99%

“…Enzymes, antibodies, microorganisms or DNA could be used as biological sensing element of biosensor in combination with an appropriate transducer (e.g., electrochemical, optical, colorimetric or piezoelectric) [19]. Wastewater treatment plant can be equipped with early warning system that includes a few toxicity measurement points placed at selected locations [4].…”

Section: Introductionmentioning

confidence: 99%

Cost Effective Method for Toxicity Screening of Pharmaceutical Wastewater Containing Inorganic Salts and Harmful Organic Compounds

Strade

Kalniņa

2019

Environmental and Climate Technologies

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Pharmaceutical wastewater biological treatment plants are stressed with multi-component wastewater and unexpected variations in wastewater flow, composition and toxicity. To avoid operational problems and reduced wastewater treatment efficiency, accurate monitoring of influent toxicity on activated sludge microorganisms is essential. This paper outlines how to predict highly toxic streams, which should be avoided, using measurements of biochemical oxygen demand (BOD), if they are made in a wide range of initial concentration. The results indicated that wastewater containing multivalent Al 3+ cations showed a strong toxic effect on activated sludge biocenosis irrespectively of dilutions, while toxicity of phenol and formaldehyde containing wastewater decreased considerably with increasing dilution. Activated sludge microorganisms were not sensitive to wastewater containing halogenated sodium salts (NaCl, NaF) and showed high treatment capacity of saline wastewater. Our findings confirm that combined indicators of contamination, such as chemical oxygen demand (COD), alone do not allow evaluating potential toxic influence of wastewater. Obtained results allow identifying key inhibitory substances in pharmaceutical wastewater and evaluating potential impact of new wastewater streams or increased loading on biological treatment system. Proposed method is sensitive and cost effective and has potential for practical implementation in multiproduct pharmaceutical wastewater biological treatment plants. Keywords -Activated sludge; biochemical oxygen demand; pharmaceutical wastewater; toxicity Nomenclature BODBiochemical oxygen demand mg/L COD Chemical oxygen demand mg/L

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A concept development of an early warning system for toxic sewage detection

Cited by 2 publications

References 8 publications

Antibiogram and beta-lactamase genes among cefotaxime resistant E. coli from wastewater treatment plant

Antibiogram and beta-lactamase genes among cefotaxime resistant E. coli from wastewater treatment plant

Cost Effective Method for Toxicity Screening of Pharmaceutical Wastewater Containing Inorganic Salts and Harmful Organic Compounds

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