A Computer-Controlled Bipolar Magnetic-Field-Gradient Driver for NMR Electrophoretic and Self-Diffusion Measurements

Accurate Anisotropic Water-Diffusion Measurements in Liquid Crystals

“…The effect of the uncertainty in the coil alignment ({0.25Њ) is scaled as those of the orthogonal Boerner (34). The impedances of the coil and the power supply were matched, providing £100 ms rise and fall times gradients and is negligible for our investigated nematic samples.…”

Determination of Oligomeric State of Proteins in Solution from Pulsed-Field-Gradient Self-Diffusion Coefficient Measurements. A Comparison of Experimental, Theoretical, and Hard-Sphere Approximated Values

“…In addition to the modern applications of PFG in also been found that self-diffusion coefficients obtained exbiomolecular NMR (6, 7), classical experimental methods perimentally as well theoretically correlate linearly with the to measure self-diffusion coefficients using PFG (8) have solvent-accessible surface area (21,22), providing an addibeen applied to proteins and DNA oligomers to understand tional parameter to represent quaternary structure of proteins. the molecular association processes (9-18).The basis of the LED-PFG sequence is the use of bipolar PFG NMR has been widely used to measure tracer diffugradient pulses (19,23,24) to minimize eddy-current effects. sion coefficients of small molecules and polymers in solution As in the conventional stimulated-echo experiment of Tanner (16).…”

On experimental aspects of electrophoretic NMR

“…A solution consisting of 1 mM sodium poly(acrylic acid) NaPAAc (Sigma-Aldrich) with mean molecular weight M w ϭ 15000, 10 mM cetyltrimethylammonium bromide (C 16 TAB, 99%, Sigma-Aldrich), and 100 mM methanol in D 2 O as solvent was used in the experiments below. The NaPAAc was received in 35 wt% solution and was dried using a freeze dryer.…”