A computer-assisted odorized hole-board for testing olfactory perception in mice

Mandairon, Nathalie; Sultan, Sébastien; Rey, Nolwen L.; Kermen, Florence; Moreno, Mélissa; Busto, Germain U.; Farget, Vincent; Messaoudi, Belkacem; Thévenet, M; Didier, Anne

doi:10.1016/j.jneumeth.2009.04.008

Cited by 37 publications

(34 citation statements)

References 39 publications

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“…Additionally, it can be speculated that the inhibition of the enzymatic browning reaction may prevent the polymerization and subsequent precipitation of volatile phenols, or other volatile compounds [27], therefore remaining free and contributing to tuber smell. In line with this speculation, previous studies have shown that not only the odor quality, but also the odor intensity (odorant concentrations) can influence olfactory preferences [11], [28]–[31]. Further profiling studies of the volatile molecules in the -PPO potatoes will provide an additional level of knowledge to better interpret our results and will help in the quest for the development of new improved cultivars.…”

Section: Discussionsupporting

confidence: 74%

“…While there was no significant difference from the null expectation for the freshly cut potato samples, there was a significant difference from the null expectation for the oxidized potato samples, suggesting that dissimilar odors are accentuated between potato lines once oxidative deterioration takes place. Additionally, to confirm our open field results, we performed a nose-poking preference experiment using a hole-board apparatus as previously described [2], [11]. Mice demonstrated significantly ( P≤0.05 ) reduced exploration times according to the ANOVA and subsequent ad hoc Newman-Keuls tests only with WT oxidized (24 h) samples, as shown in Figure 3E.…”

Section: Resultssupporting

confidence: 67%

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Improvement of Aroma in Transgenic Potato As a Consequence of Impairing Tuber Browning

et al. 2010

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Sensory analysis studies are critical in the development of quality enhanced crops, and may be an important component in the public acceptance of genetically modified foods. It has recently been established that odor preferences are shared between humans and mice, suggesting that odor exploration behavior in mice may be used to predict the effect of odors in humans. We have previously found that mice fed diets supplemented with engineered nonbrowning potatoes (-PPO) consumed more potato than mice fed diets supplemented with wild-type potatoes (WT). This prompted us to explore a possible role of potato odor in mice preference for nonbrowning potatoes. Taking advantage of two well established neuroscience paradigms, the “open field test” and the “nose-poking preference test”, we performed experiments where mice exploration behavior was monitored in preference assays on the basis of olfaction alone. No obvious preference was observed towards -PPO or WT lines when fresh potato samples were tested. However, when oxidized samples were tested, mice consistently investigated -PPO potatoes more times and for longer periods than WT potatoes. Congruently, humans discriminated WT from -PPO samples with a considerably better performance when oxidized samples were tested than when fresh samples were tested in blind olfactory experiments. Notably, even though participants ranked all samples with an intermediate level of pleasantness, there was a general consensus that the -PPO samples had a more intense odor and also evoked the sense-impression of a familiar vegetable more often than the WT samples. Taken together, these findings suggest that our previous observations might be influenced, at least in part, by differential odors that are accentuated among the lines once oxidative deterioration takes place. Additionally, our results suggest that nonbrowning potatoes, in addition to their extended shelf life, maintain their odor quality for longer periods of time than WT potatoes. To our knowledge this is the first report on the use of an animal model applied to the sensory analysis of a transgenic crop.

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Section: Discussionsupporting

confidence: 74%

Section: Resultssupporting

confidence: 67%

Improvement of Aroma in Transgenic Potato As a Consequence of Impairing Tuber Browning

et al. 2010

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“…Of the odorants used in these experiments, TMT, 2-MB, 2-PT, MMB, and IPA have been shown to drive innate aversive or avoidance responses (Kobayakawa et al, 2007; Root et al, 2014). 2,3-butanedione, geraniol, 2-PE, peanut oil and estrus female urine have been previously shown to elicit approach and appetitive behaviors (Mandairon et al, 2009; Root et al, 2014). For the natural odor panel, two distinct odor classifications were adopted.…”

Section: Star Methodsmentioning

confidence: 99%

Population Coding in an Innately Relevant Olfactory Area

Iurilli

Datta

2017

Neuron

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Different olfactory cortical regions are thought to harbor distinct sensory representations, enabling each area to play a unique role in odor perception and behavior. In the piriform cortex (PCx), spatially-dispersed sensory inputs evoke activity in distributed ensembles of neurons that act as substrates for odor learning. In contrast, the posterolateral cortical amygdala (plCoA) receives hardwired inputs that link specific odor cues to innate olfactory behaviors. Here we show that, despite stark differences in the patterning of plCoA and PCx inputs, odor-evoked neural ensembles in both areas are equally capable of discriminating odors, and exhibit similar odor tuning, reliability and correlation structure. These results demonstrate that brain regions mediating odor-driven innate behaviors can, like brain areas involved in odor learning, represent odor objects using distributive population codes; these findings suggest both alternative mechanisms for the generation of innate odor-driven behaviors and additional roles for the plCoA in odor perception.

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“…The amount of time the pup spent investigating each short arm is recorded. Another interesting device has been designed to automatically record the choice behavior of adult mice [ 99 ]. It is constituted of a holeboard (40 × 40 cm) in which fi ve holes are opened to receive glass vessels in which odorized swabs are placed and covered with bedding material.…”

Section: Visual Preweaning Mouse Pups (Pnd 14-21)mentioning

confidence: 99%

“…Depending on the test 2-5 holes can be made accessible to the tested animal, so that different paradigms can be applied, including detection tasks (in varying the concentrations of the odorant), discrimination tasks (using a habituation-dishabituation procedure), memory tasks, and preference tasks. When the mouse introduces its muzzle into a hole, sensors connected to a computer are triggered to time duration of sniffi ng and digging activity [ 99 ]. So far, this apparatus has not been used with young mice, but it holds promise to investigate the development of differential responsiveness to social odors.…”

Section: Visual Preweaning Mouse Pups (Pnd 14-21)mentioning

confidence: 99%

Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female Mice

Brock

Bakker

Baum

2013

Methods in Molecular Biology

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A computer-assisted odorized hole-board for testing olfactory perception in mice

Cited by 37 publications

References 39 publications

Improvement of Aroma in Transgenic Potato As a Consequence of Impairing Tuber Browning

Improvement of Aroma in Transgenic Potato As a Consequence of Impairing Tuber Browning

Population Coding in an Innately Relevant Olfactory Area

Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female Mice

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