In this paper, we analyze the impact on isotopic composition of spent nuclear fuel VVER-1000, due to various operational conditions, such as concentration of boric acid dissolved in water, the temperature of the fuel, and others. In addition, the impact that is caused by the technological allowances applied while manufacturing fuel assemblies that were analyzed by the mass fuel and its enrichment. The calculations were performed on models of the fuel assemblies of reactor VVER-1000. The basis was taken of a typical fuel Assembly of the Russian TVEL suppliers and the new fuel assemblies of the American company Westinghouse.