A Computational Pipeline for Accurate Prioritization of Protein‐Protein Binding Candidates in High‐Throughput Protein Libraries
Arup Mondal,
Bhumika Singh,
Roland H. Felkner
et al.
Abstract:IIdentifying the interactome for a protein of interest is challenging due to the large number of possible binders. High‐throughput experimental approaches narrow down possible binding partners but often include false positives. Furthermore, they provide no information about what the binding region is (e.g., the binding epitope). We introduce a novel computational pipeline based on an AlphaFold2 (AF) Competition Binding Assay (AF‐CBA) to identify proteins that bind a target of interest from a pull‐down experime… Show more
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