A Comprehensive Toolbox to Analyze Enhancer–Promoter Functions

Enhancer-promoter communication: unraveling enhancer strength and positioning within a given topologically associating domain (TAD)

“… 4 The relationship between enhancers and TADs was and is still intensely studied and whole novel toolbox of techniques is available to study enhancer functions. 5 …”

“…4 The relationship between enhancers and TADs was and is still intensely studied and whole novel toolbox of techniques is available to study enhancer functions. 5 In this context, Zuin et al developed an unbiased experimental strategy in mouse embryonic stem cells (ESCs) to investigate how genomic distance between a given enhancer element and its cognate TSS affects transcriptional output within a specific TAD. 1 For this purpose, a transgene was inserted on chromosome 15 within a TAD that does not contain any gene or active enhancer.…”