A comprehensive survey of the laminins and collagens type IV expressed in mouse Leydig cells and their regulation by LH/hCG

Mazaud-Guittot, Séverine; Verot, Adélie; Odet, Fanny; Chauvin, Marie-Agnès; Magueresse-Battistoni, Brigitte Le

doi:10.1530/rep-07-0561

Cited by 17 publications

(9 citation statements)

References 46 publications

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“…1A, green and red arrowheads respectively), which is in agreement with previous studies. It is known that collagens including Col IV are expressed by Leydig cells in the mouse testis 33 and endothelial cells as well as basement membrane of microvessels 34 - 37 . Next, we investigated the upstream protease(s) that could possibly cleave the NC1 domain from Col IV in the basement membrane.…”

Section: Resultsmentioning

confidence: 99%

NC1 domain of collagen α3(IV) derived from the basement membrane regulates Sertoli cell blood-testis barrier dynamics

Wong

Cheng

2013

Spermatogenesis

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The blood-testis barrier (BTB) is an important ultrastructure for spermatogenesis. Delay in BTB formation in neonatal rats or its irreversible damage in adult rats leads to meiotic arrest and failure of spermatogonial differentiation beyond type A. While hormones, such as testosterone and FSH, are crucial to BTB function, little is known if there is a local regulatory mechanism in the seminiferous epithelium that modulates BTB function. Herein, we report that collagen α3(IV) chain, a component of the basement membrane in the rat testis, could generate a noncollagenous (NC1) domain peptide [Colα3(IV) NC1] via limited proteolysis by matrix metalloproteinase-9 (MMP-9), and that the expression of MMP-9 was upregulated by TNFα. While recombinant Colα3(IV) NC1 protein produced in E. coli failed to perturb Sertoli cell tight junction (TJ)-permeability barrier function, possibly due to the lack of glycosylation, Colα3(IV) NC1 recombinant protein produced in mammalian cells and purified to apparent homogeneity by affinity chromatography was found to reversibly perturb the Sertoli cell TJ-barrier function. Interestingly, Colα3(IV) NC1 recombinant protein did not perturb the steady-state levels of several TJ- (e.g., occludin, CAR, JAM-A, ZO-1) and basal ectoplasmic specialization- (e.g., N-cadherin, α-catenin, β-catenin) proteins at the BTB but induced changes in protein localization and/or distribution at the Sertoli cell-cell interface in which these proteins moved from the cell surface into the cell cytosol, thereby destabilizing the TJ function. These findings illustrate the presence of a local regulatory axis known as the BTB-basement membrane axis that regulates BTB restructuring during spermatogenesis.

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Section: Resultsmentioning

confidence: 99%

NC1 domain of collagen α3(IV) derived from the basement membrane regulates Sertoli cell blood-testis barrier dynamics

Wong

Cheng

2013

Spermatogenesis

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“…Briefly, first‐strand cDNAs were synthesized from 1 μg of total RNA in the presence of 100 U of Superscript II (Invitrogen, Eragny, France) and a mixture of random hexamers and oligo(dT) primers (Promega, Charbonnieres, France). Real‐time PCR assays were performed in duplicates for each sample with a Rotor‐Gene 6000 (Corbett Research, Mortlake, NSW, Australia), as described elsewhere (Mazaud Guittot et al. , 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Melting curve analyses were performed immediately following the final PCR cycle to verify the specificity of the PCR product by checking its Tm. Rpl19 (ribosomal protein L19), and Gusb (glucuronidase beta) genes were chosen as references for normalizing target gene s in the testis (Mazaud Guittot et al. , 2008) and liver (unpublished data from the laboratory) respectively.…”

Section: Methodsmentioning

confidence: 99%

The effects of an in utero exposure to 2,3,7,8‐tetrachloro‐dibenzo‐p‐dioxin on male reproductive function: identification of Ccl5 as a potential marker

et al. 2010

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2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and dioxin-like compounds are widely encountered toxic substances suspected of interfering with the endocrine systems of humans and wildlife, and of contributing to the loss of fertility. In this study, we determined the changes in testicular gene expression caused by in utero exposure to TCDD along with the intra-testicular testosterone levels, epididymal sperm reserves, daily sperm production (DSP) and testis histology. To this purpose, female pregnant Sprague–Dawley rats orally received TCDD (10, 100 or 200 ng/kg body weight) or vehicle at embryonic day 15, and the offspring was killed throughout development. Hepatic Cyp1a1 gene expression was measured in the offspring to confirm the exposure to TCDD. The gross histology of the testes and intra-testicular testosterone levels were normal among the studied groups. Sperm reserves were altered in 67-day-old rats of the TCDD-200 group, but not in 145-day-old animals or in the other TCDD-exposed groups. Nonetheless, fertility was not altered in males of the TCDD-200 group, and the F2 males generated had normal sperm reserves and DSP. Microarray analysis permitted the identification of eight differentially expressed genes in the 4-week-old testes of the TCDD-200 compared with that of the control group (cut-off value ± 1.40), including the down-regulated chemokine Ccl5/Rantes. Inhibition of Ccl5/Rantes gene expression was observed throughout development in the TCDD-200 group, and at 67 and 145 days in the TCDD-100 group (animals of younger ages were not examined). Ccl5/Rantes gene expression was mostly confined in Leydig cells. F2 males generated from males of the TCDD-200 group had normal levels of Ccl5/Rantes in testis and Cyp1a1 in liver, which might indicate that Ccl5/Rantes is a marker of TCDD exposure in testis such as Cyp1a1 in liver. In conclusion, we demonstrated a decrease in Ccl5/Rantes RNA levels and a transitory decline in sperm reserves in the testes of rats of TCDD-dosed dams.

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“…More specifically, these chains have only been identified in the post‐natal period in rats when the basement membranes are more mature (Mazaud Guittot et al. ). Fröjdman et al.…”

Section: Discussionmentioning

confidence: 99%

“…Such synthesis would be related to the control of steroidogenesis in which the α 1 and α 2 collagen chains appear to be structural in nature, while collagen α 3 α 4 α 5 and α 5 α 5 α 6 are regulated by gonadotropins in normal Leydig cells and therefore not in developing Leydig cells (Mazaud Guittot et al. ).…”

Section: Discussionmentioning

confidence: 99%

Changes in Testicular Interstitial Connective Tissue of Hamsters (Mesocricetus auratus) During Ageing and After Exposure to Short Photoperiod

Beltrán-Frutos

Seco-Rovira

Ferrer

et al. 2015

Reprod Domestic Animals

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The testicular interstitium of Syrian hamster (Mesocricetus auratus) was studied during ageing and in testicular regression after exposure to a short photoperiod, in relation to the interstitial cells and their connective tissue. This tissue was assessed histochemically using Masson's trichrome technique and the expression of Heat Shock Protein 47 (HSP-47) and collagen IV (α5) was assessed in Leydig cells. Finally, an ultrastructural analysis of some cells of the testicular interstitium was made. Leydig cells were positive for HSP-47 and collagen IV (α5). Ageing did not change the parameters studied while the short photoperiod altered the synthetic activity of Leydig cells. The positivity index of these cells for HSP-47 was significantly higher in the regressed testis, but was lower for collagen IV (α5). During ageing no change were observed. Ultrastructural Leydig cells showed a discontinuous basal lamina that did not change during ageing. The basal lamina was not identified in Leydig cells regressed by exposure to a short photoperiod. In conclusion; the intertubular connective tissue suffers little change with age. By contrast, in the testis regressed after exposure to a short photoperiod the studied parameters related to the intertubular connective tissue were altered. These changes are probably related with the low synthetic activity of regressed Leydig cell.

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A comprehensive survey of the laminins and collagens type IV expressed in mouse Leydig cells and their regulation by LH/hCG

Cited by 17 publications

References 46 publications

NC1 domain of collagen α3(IV) derived from the basement membrane regulates Sertoli cell blood-testis barrier dynamics

NC1 domain of collagen α3(IV) derived from the basement membrane regulates Sertoli cell blood-testis barrier dynamics

The effects of an in utero exposure to 2,3,7,8‐tetrachloro‐dibenzo‐p‐dioxin on male reproductive function: identification of Ccl5 as a potential marker

Changes in Testicular Interstitial Connective Tissue of Hamsters (Mesocricetus auratus) During Ageing and After Exposure to Short Photoperiod

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