A comprehensive study to evaluate the wound healing potential of okra (Abelmoschus esculentus) fruit

Sipahi, Hande; Orak, Duygu; Reıs, Rengin; Yalman, Kübra; Şenol, Onur; Palabıyık, Şaziye Sezin; Deniz, İnci; Algül, Derya; Güzelmeriç, Etil; Celep, Mehmet Engin; Argın, Sanem; Özkan, Ferda; Halıcı, Zekai; Aydın, Ahmet; Yeşilada, Erdem

doi:10.1016/j.jep.2021.114843

Cited by 16 publications

(10 citation statements)

References 53 publications

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“…Author details 1 College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400716, China. 2 • support for research data, including large and complex data types • gold Open Access which fosters wider collaboration and increased citations maximum visibility for your research: over 100M website views per year…”

Section: Supplementary Informationmentioning

confidence: 99%

“…Okra ( Abelmoschus esculentus L. Moench) belongs to the family Malvaceae and is an economic crop that is cultivated throughout the world in tropical, subtropical, and temperate regions [ 1 ]. As an annual vegetable and a medicinal source, okra has attracted much attention due to its high nutritional value and health benefits for human beings [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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The complete mitochondrial genome of okra (Abelmoschus esculentus): using nanopore long reads to investigate gene transfer from chloroplast genomes and rearrangements of mitochondrial DNA molecules

et al. 2022

BMC Genomics

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Background Okra (Abelmoschus esculentus L. Moench) is an economically important crop and is known for its slimy juice, which has significant scientific research value. The A. esculentus chloroplast genome has been reported; however, the sequence of its mitochondrial genome is still lacking. Results We sequenced the plastid and mitochondrial genomes of okra based on Illumina short reads and Nanopore long reads and conducted a comparative study between the two organelle genomes. The plastid genome of okra is highly structurally conserved, but the mitochondrial genome of okra has been confirmed to have abundant subgenomic configurations. The assembly results showed that okra’s mitochondrial genome existed mainly in the form of two independent molecules, which could be divided into four independent molecules through two pairs of long repeats. In addition, we found that four pairs of short repeats could mediate the integration of the two independent molecules into one complete molecule at a low frequency. Subsequently, we also found extensive sequence transfer between the two organelles of okra, where three plastid-derived genes (psaA, rps7 and psbJ) remained intact in the mitochondrial genome. Furthermore, psbJ, psbF, psbE and psbL were integrated into the mitochondrial genome as a conserved gene cluster and underwent pseudogenization as nonfunctional genes. Only psbJ retained a relatively complete sequence, but its expression was not detected in the transcriptome data, and we speculate that it is still nonfunctional. Finally, we characterized the RNA editing events of protein-coding genes located in the organelle genomes of okra. Conclusions In the current study, our results not only provide high-quality organelle genomes for okra but also advance our understanding of the gene dialogue between organelle genomes and provide information to breed okra cultivars efficiently.

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Section: Supplementary Informationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The complete mitochondrial genome of okra (Abelmoschus esculentus): using nanopore long reads to investigate gene transfer from chloroplast genomes and rearrangements of mitochondrial DNA molecules

et al. 2022

BMC Genomics

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“…Abelmoschus esculentus is an herbal medicine used to treat wound healing with antioxidant, antibacterial, and anti-inflammation properties (Nesa et al 2014 ). This is due to the phenolic compounds like saponin, tannin, alkaloid, and flavonoid in the fruit (Sipahi et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

CSMed® wound dressing for prophylaxis and management of radiation dermatitis in breast and head–neck cancer patients: a single hospital prospective clinical trial

Lee,

Tseng,

Yang

et al. 2024

J Cancer Res Clin Oncol

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Purpose CSMed® wound dressing, a dressing with various herb extracts, was tested for its therapeutic effect in radiation dermatitis of breast and head-and-neck cancer patients. Methods This study included 20 breast cancer patients and 10 head-and-neck cancer patients. Half of the irradiated area was covered with CSMed® and the other half was under routine treatment. The severity of radiation dermatitis was evaluated with radiation therapy oncology group (RTOG) grade throughout the treatment and the follow-up period. The RTOG grade between the dressed and undressed area were compared to illustrate the therapeutic effect of CSMed® dressing. Results The results showed that CSMed® dressed area had significant lower RTOG score at 3–7 weeks and final record during the treatment, and 1–3 weeks during follow-up than undressed area. Conclusions This indicated that CSMed® can delay the onset, reduce the severity, and enhance healing of radiation dermatitis. CSMed® can be used for prophylaxis and management of radiation dermatitis.

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“…The optical density was measured at 570 nm wavelengths using a microplate reader (Multiskan Ascent; ThermoFischer). The percentage of cell viability was detected by using the following equation (Sipahi et al 2022):…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

Secondary metabolites from Gentiana cruciata L. and their anti-inflammatory and analgesic activities

Konya

Reıs

Sipahi

et al. 2022

Natural Product Research

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A previously unreported secoiridoid glycoside, cruciatoside (1) was isolated from the aerial parts of Gentiana cruciata L. along with ten known compounds eustomoside (2), eustomorusside (3), gentiopicroside (4), 6'-O-β-D-glucopyranosyl gentiopicroside (5), loganic acid (6), isoorientin (7), isovitexin (8), isovitexin 2''-(E)-ferulate (9), mangiferin (10), and 2-methyl-inositol (11). The chemical structures of the isolates were elucidated based on extensive 1D and 2D NMR experiments as well as HRMS analysis. All isolates were evaluated for their in vitro anti-inflammatory and analgesic activities. Compounds 9, 4, and 7 (200 M) showed moderate anti-inflammatory activity by inhibiting nitrite production from LPS-induced RAW 264.7 macrophage cells, with the inhibition rates of 39.5%, 25.8% and 22.9% respectively without exhibiting substantial cytotoxicity.Besides, 1, 2, 4, and 7 exerted the highest decrease in IL-6 levels. Moreover, compound 4 showed in vitro analgesic activity by decreasing the PGE2 level comparable to the reference drugs.

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A comprehensive study to evaluate the wound healing potential of okra (Abelmoschus esculentus) fruit

Cited by 16 publications

References 53 publications

The complete mitochondrial genome of okra (Abelmoschus esculentus): using nanopore long reads to investigate gene transfer from chloroplast genomes and rearrangements of mitochondrial DNA molecules

The complete mitochondrial genome of okra (Abelmoschus esculentus): using nanopore long reads to investigate gene transfer from chloroplast genomes and rearrangements of mitochondrial DNA molecules

CSMed® wound dressing for prophylaxis and management of radiation dermatitis in breast and head–neck cancer patients: a single hospital prospective clinical trial

Secondary metabolites from Gentiana cruciata L. and their anti-inflammatory and analgesic activities

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