A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat

Al-Mahmood, Sinan Mohammed Abdullah; Razak, Tariq Abdul; Abdullah, Shahrin Tarmizi Che; Na, Nik Nur Fatnoon; Mohamed, Ashgar Ali Ali; Al-Ani, Imad Matloub Dally

doi:10.7454/msk.v20i2.5889

Cited by 6 publications

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, in the case of spleen histology, marginal zone degeneration was observed due to high blood glucose. 85,86 Therefore, the observed pathological changes were not significant with the DTsiANp-cRGD/ HDAC4 treated DN mice group. Hence, STZ induced acute diabetes showed some extent of pathological changes in the lungs, liver, and spleen and remained even after the treatment with the nanoplex.…”

Section: Resultsmentioning

confidence: 83%

Cyclo-RGD Truncated Polymeric Nanoconstruct with Dendrimeric Templates for Targeted HDAC4 Gene Silencing in a Diabetic Nephropathy Mouse Model

et al. 2020

View full text Add to dashboard Cite

Diabetic nephropathy (DN), a chronic progressive kidney disease, is a significant complication of diabetes mellitus. Dysregulation of the histone deacetylases (HDACs) gene has been implicated in the pathogenesis of DN. Hence, the HDAC-inhibitors have emerged as a critical class of therapeutic agents in DN; however, the currently available HDAC4-inhibitors are mostly nonselective in nature as well as inhibit multiple HDACs. RNA interference of HDAC4 (HDAC4 siRNA) has shown immense promise, but the clinical translation has been impeded due to lack of a targeted, specific, and in vivo applicable delivery modality. In the present investigation, we examined Cyclo(RGDfC) (cRGD) truncated polymeric nanoplex with dendrimeric templates for targeted HDAC4 Gene Silencing. The developed nanoplex exhibited enhanced encapsulation of siRNA and offered superior protection against serum RNase nucleases degradation. The nanoplex was tested on podocytes (in vitro), wherein it showed selective binding to the αvβ3 integrin receptor, active cellular uptake, and significant in vitro gene silencing. The in vivo experiments showed remarkable suppression of the HDAC4 and inhibition in the progression of renal fibrosis in the Streptozotocin (STZ) induced DN C57BL/6 mice model. Histopathological and toxicological studies revealed nonsignificant abnormality/toxicity with the nanoplex. Conclusively, nanoplex was found as a promising tactic for targeted therapy of podocytes and could be extended for other kidney-related ailments.

show abstract

Section: Resultsmentioning

confidence: 83%

Cyclo-RGD Truncated Polymeric Nanoconstruct with Dendrimeric Templates for Targeted HDAC4 Gene Silencing in a Diabetic Nephropathy Mouse Model

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Studies illustrated the effects of diabetes on the adrenal gland showed that diabetes causes severe degeneration within the cells of zona glomerulosa and zona fasciculate of adrenal cortex [6,7] .…”

Section: Introductionmentioning

confidence: 99%

The possible ameliorating effect of barley grain on the histological structure of the adrenal cortex in streptozotocin (STZ)-induced diabetes in adult male albino rats

Soliman

Noya

2019

Egyptian Journal of Histology

View full text Add to dashboard Cite

Background: Diabetes is one of the most common endocrine disorders caused by lack of, or reduced insulin action leading to increase in blood glucose level and metabolic disorders in the body. Diabetes causes severe degeneration within the cells of adrenal cortex. Barley grain is known to be beneficial in regulating sugar, insulin, and cholesterol responses to foods. However, studies about its effect on the structure of adrenal cortex in diabetes are limited. Objective: This work aimed to evaluate the possible protective effect of barley grain on the histological changes within the adrenal cortex in streptozotocin-induced diabetes in adult male albino rats. Materials and Methods: Forty adult male albino rats were used in the study. They were divided into four groups of ten each: group I was the control group; group II received barley grain extract intragastrically; group III included rats with streptozotocin (STZ)-induced diabetes; and group IV received barley green extract after inducing diabetes. After 12 weeks, Rats were sacrificed by using ether anesthesia. Blood plasma was collected for hormonal analysis then adrenal tissue was used for histological and immunohistochemical studies. Results: STZ-induced diabetes resulted in a significant decrease in body weight and serum aldosterone and corticostrione levels and significant increase in adrenal weight. The cells of adrenal cortex appeared disorganized. Their nuclei appeared pyknotic with vacuolated cytoplasm. The blood sinusoids in-between the cells of adrenal cortex appeared dilated and congested. Distorted smooth endoplasmic reticulum and degenerated (mouse-bitten) mitochondria were also detected within the cells. Administration of barley grain after induction of diabetes led to amelioration of these changes. Conclusion: Barley grain extract administration improved the histological and biochemical changes induced by diabetes in adrenal cortex.

show abstract

Antidiabetic activity of Physalis angulata L. fruit juice on streptozotocin-induced diabetic rats

Iwansyah¹,

Luthfiyanti

Ardiansyah

et al. 2022

South African Journal of Botany

View full text Add to dashboard Cite

A comprehensive study of chronic diabetes complications in streptozotocin-induced diabetic rat

Cited by 6 publications

References 14 publications

Cyclo-RGD Truncated Polymeric Nanoconstruct with Dendrimeric Templates for Targeted HDAC4 Gene Silencing in a Diabetic Nephropathy Mouse Model

Cyclo-RGD Truncated Polymeric Nanoconstruct with Dendrimeric Templates for Targeted HDAC4 Gene Silencing in a Diabetic Nephropathy Mouse Model

The possible ameliorating effect of barley grain on the histological structure of the adrenal cortex in streptozotocin (STZ)-induced diabetes in adult male albino rats

Antidiabetic activity of Physalis angulata L. fruit juice on streptozotocin-induced diabetic rats

Contact Info

Product

Resources

About