A Comprehensive Structure–Function Analysis of<i>Arabidopsis</i>SNI1 Defines Essential Regions and Transcriptional Repressor Activity

Mosher, Rebecca A.; Durrant, Wendy E.; Wang, Dong; Song, Junqi; Dong, Xinnian

doi:10.1105/tpc.105.039677

Cited by 123 publications

(139 citation statements)

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“…and SNI1 was found to encode a Leu-rich nuclear transcriptional repressor protein (Li et al, 1999;Mosher et al, 2006). Similarly, rfc3-1 npr1 double mutant plants exhibit similar phenotypes as rfc3-1, suggesting that, like SNI1, RFC3 is a negative regulator of SAR and that this function of RFC3 does not require NPR1.…”

Section: Discussionmentioning

confidence: 88%

“…SNI1 was identified earlier as a negative regulator of SAR (Li et al, 1999;Mosher et al, 2006). A mutation in SNI1 causes up-regulation of defense genes and hypersensitivity to the defense signal molecule SA.…”

Section: Discussionmentioning

confidence: 99%

“…Accession numbers are as follows: AtRFC3, NP_177871.1; OsRFC3, XM_468050.1; ScRFC3, NC_001146.3; DmRFC3, NM_135555.2; hRFC5, NM_007370.3; MmRFC5, Q9D0F6; XRFC5, BC072889.1; and DrRFC5, NM_001003862.1. (Mosher et al, 2006). Loss of SNI1 function leads to increased abundance of activating histone modifications such as acetylated histone H3 and methylated Lys-4 on histone H3 at the PR-1 promoter, which may make the chromatin at the promoter region adopt a more accessible conformation and lead to elevated gene expression (Mosher et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, an INA hypersensitive mutant, suppressor of npr1-1, inducible1 (sni1), was found to be a transcriptional repressor of PR gene expression that negatively regulates SAR (Li et al, 1999;Mosher et al, 2006). Mutant plants of sni1 exhibit a basal level of PR gene expression that is independent of NPR1, and this expression is further enhanced upon SAR induction.…”

mentioning

confidence: 99%

“…SNI1 encodes a protein with structural similarity to Armadillo repeat proteins potentially involved in scaffolding or protein-protein interactions. Chromatin immunoprecipitation experiments indicated that histone modification could be involved in SNI1 function (Mosher et al, 2006). When a genetic screen was conducted to search for genetic suppressors of sni1, it was found that a loss-of-function mutation in RAD51D suppresses sni1 phenotypes completely.…”

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confidence: 99%

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Negative Regulation of Systemic Acquired Resistance by Replication Factor C Subunit3 in Arabidopsis

et al. 2009

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Systemic acquired resistance (SAR) is a plant immune response induced by local necrotizing pathogen infections. Expression of SAR in Arabidopsis (Arabidopsis thaliana) plants correlates with accumulation of salicylic acid (SA) and up-regulation of Pathogenesis-Related (PR) genes. SA is an essential and sufficient signal for SAR. In a genetic screen to search for negative regulators of PR gene expression and SAR, we found a new mutant that is hypersensitive to SA and exhibits enhanced induction of PR genes and resistance against the virulent oomycete Hyaloperonospora arabidopsidis Noco2. The enhanced pathogen resistance in the mutant is Nonexpressor of PR genes1 independent. The mutant gene was identified by map-based cloning, and it encodes a protein with high homology to Replication Factor C Subunit3 (RFC3) of yeast and other eukaryotes; thus, the mutant was named rfc3-1. rfc3-1 mutant plants are smaller than wild-type plants and have narrower leaves and petals. On the epidermis of true leaves, there are fewer cells in rfc3-1 compared with the wild type. Cell production rate is reduced in rfc3-1 mutant roots, indicating that the mutated RFC3 slows down cell proliferation. As Replication Factor C is involved in replication-coupled chromatin assembly, our data suggest that chromatin assembly and remodeling may play important roles in the negative control of PR gene expression and SAR.

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Section: Discussionmentioning

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Negative Regulation of Systemic Acquired Resistance by Replication Factor C Subunit3 in Arabidopsis

et al. 2009

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Systemic Signalling in Plant Defence

Helliwell

Yang

2009

Encyclopedia of Life Sciences

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Systemic signalling involves a complex network of signal transduction and amplification that leads to the activation of defence genes and establishment of systemic resistance throughout the entire plant. On microbial invasion, pathogen‐associated molecular patterns and effectors are frequently detected and recognized by plant receptors. Localized perception at the infection site rapidly triggers a cascade of early signalling events such as ion fluxes, protein phosphorylation and production of reactive oxygen species. Subsequently, secondary signal molecules are synthesized and involved in amplification of defence signalling and the establishment of systemic acquired resistance. In addition, crosstalks often occur among various signalling pathways, which further modulate host defence in response to different types of pathogen attack. Key concepts: Local resistance to pathogen infection generally results from PAMP (pattern‐associated molecular pattern)‐triggered immunity, otherwise known as basal defence, and microbial effector‐triggered immunity. Microbial infection of local tissues often leads to systemic acquired resistance in distal tissues which provides long‐lasting broad‐spectrum resistance to a variety of pathogens. Mobile signals for systemic acquired resistance are translocated via vasculature and may include small peptides such as AtPep1, methyl salicylate, jasmonate, azelaic acid and other lipid‐derived molecules. Both local and systemic resistance involve a complex network of signal transduction which includes extensive crosstalks among reactive oxygen species (e.g. hydrogen peroxide and nitric oxide), salicylic acid, jasmonic acid, ethylene, abscisic acid, auxin and MAP kinase signalling pathways.

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A guide to the integrated application of on‐line data mining tools for the inference of gene functions at the systems level

Meier

Gehring

2008

Biotechnology Journal

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Genes function in networks to achieve a common biological response. Thus, inferences into the biological role of individual genes can be gained by analyzing their association with other genes with more precisely defined functions. Here, we present a guide, using the well-characterized Arabidopsis thaliana pathogenesis-related protein 2 gene (PR-2) as an example, to document how the sequential use of web-based tools can be applied to integrate information from different databases and associate the function of an individual gene with a network of genes and additionally identify specific biological processes in which they collectively function. The analysis begins by performing a global expression correlation analysis to build a functionally associated gene network. The network is subsequently analyzed for Gene Ontology enrichment, stimuli and mutant-specific transcriptional responses and enriched putative promoter regulatory elements that may be responsible for their correlated relationships. The results for the PR-2 gene are entirely consistent with the published literature documenting the accuracy of this type of analysis. Furthermore, this type of analysis can also be performed on other organisms with the appropriate data available and will greatly assist in understanding individual gene functions in a systems context.

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A Comprehensive Structure–Function Analysis ofArabidopsisSNI1 Defines Essential Regions and Transcriptional Repressor Activity

Cited by 123 publications

References 72 publications

Negative Regulation of Systemic Acquired Resistance by Replication Factor C Subunit3 in Arabidopsis

Negative Regulation of Systemic Acquired Resistance by Replication Factor C Subunit3 in Arabidopsis

Systemic Signalling in Plant Defence

A guide to the integrated application of on‐line data mining tools for the inference of gene functions at the systems level

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