A comprehensive strategy for quality evaluation of Wushe Zhiyang Pills by integrating UPLC-DAD fingerprint and multi-ingredients rapid quantitation with UPLC-MS/MS technology

Jiang, Maoyuan; Cao, Jiliang; Zhang, Chunbo; Su, Biru; Wang, Shengpeng; Ning, Na; Lei, Ting

doi:10.1016/j.jpba.2021.114556

Cited by 11 publications

(9 citation statements)

References 27 publications

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“…To date, researchers have conducted extensive studies on the detailed composition of herbal medicines (HMs) or THFs using various analytical techniques, such as highperformance capillary electrophoresis, high-performance liquid chromatography (HPLC), ultra-performance liquid chromatography (UPLC), UPLC-tandem mass spectrometry (MS/MS), and gas chromatography-mass spectrometry [10][11][12][13][14][15][16]. Among these techniques, HPLC or LC-MS/MS techniques have been the most widely applied to the quality control and chemical profiling of HMs or THFs.…”

Section: Introductionmentioning

confidence: 99%

Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry for Simultaneous Analysis of 22 Analytes of Oncheong-Eum, a Traditional Korean Herbal Formula

Seo,

Shin

2023

Processes

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Oncheong-eum (OCE) is a traditional Korean herbal formula comprising eight medicinal herbs for treating skin disorders, including eczema and skin rashes. Here, we sought to simultaneously analyze 22 analytes of OCE using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). All analytes were separated on a Waters Acquity UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) maintained at 45 °C by gradient elution with a mobile phase of 0.1% (v/v) aqueous formic acid–acetonitrile. By applying a multiple reaction monitoring method, we rapidly determined the various analytes simultaneously. The coefficient of determination of the regression equation prepared in the tested concentration range of each authentic reference standard was ≥0.9950 and showed good linearity. The accuracy ranged from 84.23% to 115.47%, and the relative standard deviation values for intra- and interday precisions ranged from 0.84% to 9.57%, respectively. Analysis of OCE samples using this method showed that they contained up to 27.10 mg/g of active ingredients. The method can provide data to improve the consistency and, thus, the future quality of OCE preparations and other traditional herbal formulas.

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Section: Introductionmentioning

confidence: 99%

Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry for Simultaneous Analysis of 22 Analytes of Oncheong-Eum, a Traditional Korean Herbal Formula

Seo,

Shin

2023

Processes

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“…Plant metabolomics is a comprehensive method that can qualitatively and quantitatively analyse metabolite data in a specific period or condition, and it is combined with chemical informatics analysis to ensure target differential metabolites [ 16 ]. UPLC-Q-TOF-MS is a widely used metabolite analysis method because of its high resolution and sensitivity [ 17 ]. ER is closely related to dysmenorrhea.…”

Section: Introductionmentioning

confidence: 99%

Mechanism and Pharmacodynamic Substance Basis of Raw and Wine-Processed Evodia rutaecarpa on Smooth Muscle Cells of Dysmenorrhea Mice

Liu

Hong

Chen

et al. 2023

Pain Research and Management

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Objectives. Evodia rutaecarpa (ER) is a well-known herbal Chinese medicine traditionally used for analgesia in dysmenorrhea, headaches, abdominal pain, etc. Notably, the analgesic effect of wine-processed Evodia rutaecarpa (PER) was more potent than that of raw ER. This research aimed to investigate the mechanism and pharmacodynamic substance basis of raw ER and PER on smooth muscle cells of dysmenorrhea mice. Methods. Metabolomics methods based on UPLC-Q-TOF-MS were utilized to analyse the differential components of ER before and after wine processing. Afterwards, the uterine smooth muscle cells were isolated from the uterine tissue of dysmenorrhea and normal mice. The isolated dysmenorrhea uterine smooth muscle cells were randomly divided into four groups: model group, 7-hydroxycoumarin group (1 mmol/L), chlorogenic acid (1 mmol/L), and limonin (50 μmol/L). The normal group consisted of the isolated normal mouse uterine smooth muscle cells, which were repeated 3 times in each group. The cell contraction and the expression of P2X3 and Ca2+ in vitro were determined using immunofluorescence staining and laser confocal; ELISA was used for detection of PGE2, ET-1, and NO content after 7-hydroxycoumarin, chlorogenic acid, and limonin administered for 24 h. Results. The metabolomics results suggested that seven differential compounds were identified in the extracts of raw ER and PER, including chlorogenic acid, 7-hydroxycoumarin, hydroxy evodiamine, laudanosine, evollionines A, limonin, and 1-methyl-2-[(z)-4-nonenyl]-4 (1H)-quinolone. The in vitro results showed that 7-hydroxycoumarin, chlorogenic acid, and limonin were able to inhibit cell contraction and PGE2, ET-1, P2X3, and Ca2+ in dysmenorrhea mouse uterine smooth muscle cells and increase the content of NO. Conclusion. Our finding suggested that the compounds of the PER were different from those of the raw ER, and 7-hydroxycoumarin, chlorogenic acid, and limonin could improve dysmenorrhea in mice whose uterine smooth muscle cell contraction was closed with endocrine factors and P2X3-Ca2+ pathway.

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“…To search for Q-markers and hypoglycemic constituents in Cyanotis arachnoidea , and examine their hypoglycemic molecular mechanisms of action through the analysis of Q-markers and biomarkers, chemical pattern recognition and network pharmacology were performed to screen differential components, identify active ingredients and predict their potential targets. The TCM fingerprint is a comprehensive and quantifiable identification method that has been widely used for TCM quality control in combination with chemical pattern recognition techniques, such as hierarchical cluster analysis (HCA) and principal component analysis (PCA) ( Jiang et al, 2022 ; Zhang et al, 2021 ). Network pharmacology is systematic and holistic and can be widely used to predict and research the multi-target mechanisms of TCM in the treatment of various diseases ( Pan et al, 2022 ; Li et al, 2021 ; Wang, Cui & Li, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of quality markers for Cyanotis arachnoidea and analysis of its physiological mechanism based on chemical pattern recognition, network pharmacology, and experimental validation

Hu,

Feng,

et al. 2023

PeerJ

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Cyanotis arachnoidea C. B. Clarke is a traditional Chinese medicinal herb that has a limited clinical use in the treatment of diabetes mellitus (DM) in minority areas of Guizhou in China. However, few prior reports are available on the quality control of Cyanotis arachnoidea, and its quality markers and hypoglycemic mechanism are still unclear. The purpose of this study is to explore the quality markers (Q-markers) of Cyanotis arachnoidea and predict its hypoglycemic mechanism. In this study, ultra-high-performance liquid chromatography (UHPLC) fingerprint combined with chemical pattern recognition were performed, and four differential components were screened out as quality markers, including 20-Hydroxyecdysone, 3-O-acetyl-20-hydroxyecdysone, Ajugasterone C, and 2-O-acetyl-20-hydroxyecdysone. Network pharmacology analysis revealed 107 therapeutic target genes of Cyanotis arachnoidea in DM treatment, and the key targets were Akt1, TNF, IL-6, MAPK3, and JUN. The hypoglycemic mode of action of Cyanotis arachnoidea may be mediated by tumor necrosis factor (TNF) signaling, cancer, insulin resistance, and JAK-STAT pathways. Molecular docking analysis disclosed that the foregoing quality markers effectively bound their key target genes. An in vitro experiment conducted on pancreatic islet β-cells indicated that the forenamed active components of Cyanotis arachnoidea had hypoglycemic efficacy by promoting PI3K/Akt and inhibiting MAPK signaling. UHPLC also accurately quantified the quality markers. The identification and analysis of quality markers for Cyanotis arachnoidea is expected to provide references for the establishment of a quality control evaluation system and clarify the material basis and hypoglycemic mechanisms of this traditional Chinese medicine (TCM).

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A comprehensive strategy for quality evaluation of Wushe Zhiyang Pills by integrating UPLC-DAD fingerprint and multi-ingredients rapid quantitation with UPLC-MS/MS technology

Cited by 11 publications

References 27 publications

Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry for Simultaneous Analysis of 22 Analytes of Oncheong-Eum, a Traditional Korean Herbal Formula

Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry for Simultaneous Analysis of 22 Analytes of Oncheong-Eum, a Traditional Korean Herbal Formula

Mechanism and Pharmacodynamic Substance Basis of Raw and Wine-Processed Evodia rutaecarpa on Smooth Muscle Cells of Dysmenorrhea Mice

Identification of quality markers for Cyanotis arachnoidea and analysis of its physiological mechanism based on chemical pattern recognition, network pharmacology, and experimental validation

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