Biodiesel production from waste frying oil as a fuel can help the recycling and contributing to the economy. Transesterification reaction is preferred, it is not because the most common technique, it is one of the most efficient ways for the biodiesel production. The transesterification parameters of waste frying oil were applied as 6:1 Methanol to oil molar ratio with NaOH catalyst 0.4 g (w/w), 57°C reaction temperature, and 60 minutes reaction time and transesterification yield 88.5%. In this study, the effect of biodiesel blends with the organicbased manganese additive on performance and exhaust emissions of a single-cylinder, fourstroke, direct injected diesel engine with air cooling system at 2200 1/min fixed engine speed and with four different engine loads was experimentally investigated. The engine loads are (BMEP, 0.12 MPa, 0.24 MPa, 0.36 MPa and 0.48 MPa), and at the beginning of the tests, the engine was warmed with No. 2 diesel fuel. The oil and inlet air temperatures were kept at 85 ±2 °C and 25 ±1 °C, respectively. The testing fuels used in the experiments were composed of waste frying oil biodiesel blended with the No. 2 diesel fuel volumetrically with the ratio of 20, 40 and 60, respectively. The results obtained from the experimental study were compared with No. 2 diesel fuel. The effects of blends on CO, THC, NOx and smoke were investigated by emission tests. Additionally, brake specific fuel consumption of test fuels was examined. By virtue of the environmental effects, biodiesel production from waste frying oil has been become significant.