A comprehensive review on enzyme-based biosensors: Advanced analysis and emerging applications in nanomaterial-enzyme linkage

Recent advances of biocompatible optical nanobiosensors in liquid biopsy: towards early non-invasive diagnosis

“…Therefore systematically optimization on buffer selection and nanoparticle stability are important basis for further application. 39 Nanosensors accurately reflect the differences between healthy individuals and patients with leukemia in real-world practice. 40 QDs loading with self-assembled DNA concatemers were tied to the surface of magnetic microspheres to quantify salivary exosomes with LOD of 500 particles per μL, which also showed good sensing capability for salivary samples of patients with oral squamous cell carcinoma.…”

“…However, optimization of reaction conditions, such as enzymatic cleavage reaction time, enzyme concentration, substrate concentration ratio, and buffer selection, needs to be systematically conducted. 39 Another study combined SERS sensing performance with the signal amplification functions of a CHA-based surface. This integrated sensing system showed advancements in simultaneous quantification of multiple miRNA targets in serum with LOD of 0.15 pM.…”

Enhancing Chymotrypsin Activity and Stability of Capillary Immobilized Enzyme Microreactors Using Zeolitic Imidazolate Frameworks as Encapsulation Materials

“…Metal–organic frameworks (MOFs) are organic–inorganic hybrid materials with intramolecular pores formed by the self-assembly of organic ligands and inorganic metal ions or clusters through coordination bonds. − Compared to conventional inorganic porous materials, − MOFs offer adjustable high porosity, high specific surface area, and a diverse range of framework compositions. − In recent years, MOFs as carriers to in situ encapsulate free enzymes by coincubation of enzymes and MOFs precursors through biomineralization have received a lot of attention from researchers. − Enzyme in situ encapsulation inside MOFs can not only significantly increase the enzyme loading amount but also facilitate the enzyme catalytic process through the substrate selectivity of MOFs pore size and enzyme microenvironment, significantly increasing enzyme activity . In addition, the rigid domain-limiting structure of the MOFs skeleton can limit the conformational changes of the enzyme, and thus MOFs can be used as an external protective layer for encapsulating the enzyme, thereby further improving the stability of the enzyme. , Inspired by these excellent works, we speculate that in situ encapsulation of enzymes in capillaries to prepare OT-IMERs may significantly reduce the influence of the external environment and complex sample matrices on enzyme activity and stability.…”

Insights into Ionic Liquids for Oil Desulfurization: A Study Based on Bibliometric Mapping

“…The possible reason for this is the rich oil variety and the large amount of literature involved, making it difficult to perform a review analysis. Bibliometrics is an emerging overview analysis, which can systematically analyze topics regarding research trends, status, and hotspots . More importantly, bibliometrics can handle a large amount of literature data .…”

“…Bibliometrics is an emerging overview analysis, 20 which can systematically analyze topics regarding research trends, status, and hotspots. 21 More importantly, bibliometrics can handle a large amount of literature data. 22 With the help of literature visualization tools, 23 existing research gaps can be identified, and emerging research ideas can be revealed more rigorously.…”