A Comprehensive Review of Dysregulated miRNAs Involved in Cervical Cancer

Sharma, G. D.; Dua, Pradeep; Agarwal, Subhash Mohan

doi:10.2174/1389202915666140528003249

Cited by 62 publications

(48 citation statements)

References 162 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…MiRNAs, a subset of short noncoding RNAs (17–22 nucleotides), could post‐transcriptionally regulate the expression of relevant genes and thus participate in the development of cervical cancer . Approximately, 70 miRNAs have been discovered to be involved in cervical cancer thus far and the list is ever growing .…”

Section: Introductionmentioning

confidence: 99%

Retracted: MicroRNA‐92a promotes tumor growth and suppresses immune function through activation of MAPK/ERK signaling pathway by inhibiting PTEN in mice bearing U14 cervical cancer

Kang

et al. 2018

Cancer Medicine

View full text Add to dashboard Cite

Cervical cancer is known as the possible outcome of genital infection, while the molecular mechanisms of initiation, development, and metastasis of cervical cancer have not yet been fully elucidated. Our study aims to investigate the effects of microRNA‐92a (miR‐92a) on tumor growth and immune function by targeting PTEN via the MAPK/ERK signaling pathway in tumor‐bearing mice. C57BL/6 female mice were used for tumor‐bearing mouse models and their tumor and adjacent normal tissues were collected, and normal cervical tissues were obtained from normal mice. Serum levels of tumor necrosis factor‐α (TNF‐α) and soluble interleukin‐2 receptor (sIL‐2R) were detected by ELISA. The cells were divided into the normal, blank, negative control (NC), miR‐92a mimic, miR‐92a inhibitor, siRNA‐PTEN, and miR‐92a inhibitor + siRNA‐PTEN groups. Dual‐luciferase reporter assay was adopted to determine the relationship between PTEN and miR‐92a. Expressions of miR‐92a, PTEN, TNF‐α, sIL‐2R, ERK1, and ERK2 were tested by RT‐qPCR and Western blotting. Cell proliferation was detected by cell count kit‐8 ( CCK‐8); cell cycle and apoptosis were detected by flow cytometry. Compared with the normal cervical tissues and adjacent normal tissues, the cervical cancer tissues exhibited increased expressions of miR‐92a, p‐ERK1/2, and serum levels of TNF‐α and sIL‐2R while decreased PTEN expression. PTEN was confirmed to be the target gene of miR‐92a. As compared with the blank and NC groups, expressions of miR‐92a, ERK1 and ERK2 increased, and expressions of PTEN decreased in the miR‐92a mimic group. The miR‐92a mimic group exhibited increased expression levels of TNF‐α and sIL‐2R, cell proliferation, and cell number in S phase but decreased cell apoptosis, and cell number in G0/G1 phase, while the miR‐92a inhibitor group followed opposite trends. miR‐92a promotes tumor growth and suppresses immune function by inhibiting PTEN via activation of the MAPK/ERK signaling pathway in mice bearing U14 cervical cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

Retracted: MicroRNA‐92a promotes tumor growth and suppresses immune function through activation of MAPK/ERK signaling pathway by inhibiting PTEN in mice bearing U14 cervical cancer

Kang

et al. 2018

Cancer Medicine

View full text Add to dashboard Cite

show abstract

“…Cervical cancer is one of the most common types of gynecologic cancer, and is the fourth leading cause of cancer-associated mortality among females worldwide (14). Previous studies have demonstrated that miRNAs may be abnormally expressed in cervical cancer and this is associated with the prognosis of cervical cancer (15,16). Zhao et al (17) validated that the expression level of miR-491-5p was significantly decreased in cervical cancer and that miR-491-5p inhibited cancer cell proliferation, primarily by regulating human telomerase reverse transcriptase.…”

Section: Mir-494 Expression Is Decreased In Cervical Cancermentioning

confidence: 99%

miR-494 inhibits cervical cancer cell proliferation through upregulation of SOCS6 expression

et al. 2017

View full text Add to dashboard Cite

Abstract. It is unclear how microRNA (miR)-494 inhibits the proliferation of cervical cancer cells by altering the expression of SOCS6. Therefore, the present study aimed to investigate the molecular mechanism underlying miR-494 regulation of suppressor of cytokine signaling 6 (SOCS6) in human cervical cancer samples and the human cervical cancer HeLa cell line. The expression of miR-494 was determined using reverse transcription-quantitative polymerase chain reaction. In addition, TargetScan was used to predict miR-494 target genes and the luciferase reporter assay was used to determine whether SOCS6 was a direct target of miR-494. The results of the present study demonstrated that compared with the cervical intraepithelial neoplasia and normal cervical tissues, the miR-494 expression level in cervical cancer samples was significantly decreased (P<0.01). In addition, compared with normal cervical tissue, miR-494 expression level was significantly decreased in cervical intraepithelial lesions (P<0.05). Furthermore, the expression of miR-494 was associated with patients with or without lymph node metastasis, clinical stage and depth of stromal invasion (P<0.01); however, miR-494 expression was not identified to be associated with age, tumor size and menopausal status (P>0.05). Transfection of a miR-494 mimic significantly increased the expression level of miR-494 in HeLa cells (P<0.01), and anti-miR-494 transfection decreased the expression of miR-494 (P<0.01). An MTT proliferation assay and Boyden chamber invasion ability assay revealed that miR-494 mimic transfection significantly inhibited the proliferation, and invasion ability of HeLa cells (P<0.01), whereas anti-miR-494 transfection significantly increased the proliferation and invasion ability (P<0.05). SOCS6 was predicted, using bioinformatics, to be the target gene of miR-494 and this was validated using a luciferase reporter assay. Western blot analysis revealed that transfection of miR-494 significantly increased the expression of SOCS6 in HeLa cells, and transfection of anti-miR-494 significantly decreased the expression of SOCS6. Therefore, the results of the present study demonstrated that miR-494 expression in cervical cancer was significantly decreased. Exhibiting a decreased expression level of miR-494 may result in enhanced proliferative and invasive abilities of HeLa cell, thus contributing to the occurrence, and development of cervical cancer. IntroductionCervical cancer is the second most common type of female malignancy, subsequent to breast cancer. There were an estimated 527,600 new cervical cancer cases and 265,700 deaths worldwide in 2012 (1). Although cervical cytology screening has enabled early detection and early treatment of cervical cancer, the incidence and mortality rates continue to increase each year (2,3). The majority of patients with cervical cancer are treated with standard radiation therapy and chemotherapy, but therapeutic response varies. Thus, studies are required to determine the pathogenesis of cervical cancer in orde...

show abstract

“…miR-21 a miR-146a [13,16]. Pro definitivní validaci vybraných miRNA bude ovšem potřeba provést více nezávislých studií a rovněž popsat přesnou roli jednotlivých miRNA v karcinogenezi indukované virem HPV.…”

Section: Genom Hpv 16unclassified

Human Papillomavirus – Role in Cervical Carcinogenesis and Methods of Detection

Bartošík¹,

Hrstka²,

Jiráková³

2018

Klin Onkol

View full text Add to dashboard Cite

Východiska: Perzistentní infekce onkogenním typem lidského papilomaviru (human papillomavirus -HPV), nejčastěji HPV 16 a HPV 18, může vést k rozvoji řady maligních onemocnění, zejména pak ke karcinomu děložního hrdla u žen. Testování na přítomnost HPV DNA se tudíž zavádí jako komplementární metoda ke stávajícímu cytologickému vyšetření, mimo jiné i díky vyšší citlivosti. Cíl: Tento přehledový článek pojednává o roli HPV v karcinogenezi děložního hrdla, zejména popisuje vznik cervikálních intraepiteliálních lézí (CIN1-3) a molekulární mechanizmus transformace buněk. Představuje existující bio markery pro dia gnostiku prekancerózních lézí, mezi které patří např. mRNA transkripty genů E6 a E7, protein p16 (inhibitor cyklin-dependentní kinázy regulující přechod z G1 do S fáze buněčného cyklu), změněné metylační vzorce DNA nebo působení specifických miRNA, tj. krátkých nekódujících jednořetězcových RNA o délce 18-22 nukleotidů, které posttranskripčně regulují genovou expresi. Dále rozebírá výhody a nevýhody komerčně dostupných HPV testů, a v neposlední řadě představuje nově vyvíjené metody umožňující levnou a rychlou dia gnostiku HPV na bázi optické i elektrochemické detekce. Závěr: Nádory děložního hrdla i navzdory velkému pokroku v posledních letech stále patří mezi nejrozšířenější ženské malignity s vysokou mortalitou, zejména v rozvojových zemích. Ke snížení mortality by mohla pomoci implementace HPV testování do rutinního screeningového vyšetření. To však bude třeba finančně zefektivnit tak, aby bylo cenově konkurenceschopné se stávajícími cytologickými vyšetřeními. Klíčová slovaHPV -karcinom cervixu -HPV testování -hybridizace nukleových kyselin -mRNA -metylace DNA -miRNA SummaryBackground: Persistent infection with high-risk human papillomavirus (HPV) strains, especially HPV 16 and HPV 18, is associated with the onset of various malignant dis eases, includ ing cervical carcinoma in women. HPV DNA test ing is thus be ing implemented as a complementary method to standard cytological examination, mainly due to its increased sensitivity. Aim: This review outlines the role of HPV in cervical carcinogenesis, with a focus on the formation of cervical intraepithelial neoplasias (CIN1-3) and the molecular mechanism underly ing cellular transformation. Current bio markers used to screen premalignant lesions are described, includ ing mRNA transcripts of the E6 and E7 genes, protein p16 (a cyclin-dependent kinase inhibitor that regulates cell cycle progression from G1 to S phase), altered DNA methylation patterns, and actions of specific microRNAs (short (18-22 bp), non-coding, single-stranded RNA molecules that regulate gene expression at the post-transcriptional level). This review also describes the advantages and drawbacks of commercial HPV tests, and depicts novel methods for more cost-effective and faster HPV dia gnostics based on optical or electrochemical detection. Conclusion: Although great progress has been made, the incidence and mortality rates of cervical malignancies remain relatively high , especially in d...

show abstract

A Comprehensive Review of Dysregulated miRNAs Involved in Cervical Cancer

Cited by 62 publications

References 162 publications

Retracted: MicroRNA‐92a promotes tumor growth and suppresses immune function through activation of MAPK/ERK signaling pathway by inhibiting PTEN in mice bearing U14 cervical cancer

Retracted: MicroRNA‐92a promotes tumor growth and suppresses immune function through activation of MAPK/ERK signaling pathway by inhibiting PTEN in mice bearing U14 cervical cancer

miR-494 inhibits cervical cancer cell proliferation through upregulation of SOCS6 expression

Human Papillomavirus – Role in Cervical Carcinogenesis and Methods of Detection

Contact Info

Product

Resources

About