A Comprehensive Proteomic Survey of ABA-Induced Protein Phosphorylation in Rice (Oryza sativa L.)

Qiu, Jian; Hou, Yuxuan; Wang, Yifeng; Li, Zhiyong; Zhao, Juan; Tong, Xiaohong; Lin, Haiyan; Wei, Xiangjin; Ao, Hejun; Zhang, Jian

doi:10.3390/ijms18010060

Cited by 32 publications

(23 citation statements)

References 61 publications

(88 reference statements)

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“…3d). Our previous phosphoproteomic study of ABA‐induced protein phosphorylation revealed that exogenous ABA enhanced the bZIP72 phosphorylation intensity on Serine 71 (Qiu et al ., 2017). Thus, we attempted to specify the residues responsible for SAPK10‐mediated phosphorylation on bZIP72 by using bZIP72 S71A as the substrate for the kinase assay.…”

Section: Resultsmentioning

confidence: 99%

Abscisic acid promotes jasmonic acid biosynthesis via a ‘SAPK10‐bZIP72‐AOC’ pathway to synergistically inhibit seed germination in rice (Oryza sativa)

et al. 2020

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Abscisic acid (ABA) and jasmonic acid (JA) both inhibit seed germination, but their interactions during this process remain elusive. Here, we report the identification of a 'SAPK10-bZIP72-AOC' pathway, through which ABA promotes JA biosynthesis to synergistically inhibit rice seed germination. Using biochemical interaction and phosphorylation assays, we show that SAPK10 exhibits autophosphorylation activity on the 177 th serine, which enables it to phosphorylate bZIP72 majorly on 71 st serine. The SAPK10-dependent phosphorylation enhances bZIP72 protein stability as well as the DNA-binding ability to the G-box cis-element of AOC promoter, thereby elevating the AOC transcription and the endogenous concentration of JA. Blocking of JA biosynthesis significantly alleviated the ABA sensitivity on seed germination, suggesting that ABA-imposed inhibition partially relied on the elevated concentration of JA. Our findings shed a novel insight into the molecular networks of ABA-JA synergistic interaction during rice seed germination.

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Section: Resultsmentioning

confidence: 99%

Abscisic acid promotes jasmonic acid biosynthesis via a ‘SAPK10‐bZIP72‐AOC’ pathway to synergistically inhibit seed germination in rice (Oryza sativa)

et al. 2020

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“…ABA has pivotal roles in plant tolerance to water deficit or drought stress [ 48 , 49 ]. Considering the differential expression of OsPHDs under ABA treatment, we also investigated their expression under water deficit stress.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the PHD-Finger Family Genes and Their Responses to Environmental Stresses in Oryza sativa L.

Sun

Jia

Yang

et al. 2017

IJMS

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The PHD-finger family has been demonstrated to be involved in regulating plant growth and development. However, little information is given for its role in environmental stress responses. Here, we identified a total of 59 PHD family genes in the rice genome. These OsPHDs genes were located on eleven chromosomes and synteny analysis only revealed nine duplicated pairs within the rice PHD family. Phylogenetic analysis of all OsPHDs and PHDs from other species revealed that they could be grouped into two major clusters. Furthermore, OsPHDs were clustered into eight groups and members from different groups displayed a great divergence in terms of gene structure, functional domains and conserved motifs. We also found that with the exception of OsPHD6, all OsPHDs were expressed in at least one of the ten tested tissues and OsPHDs from certain groups were expressed in specific tissues. Moreover, our results also uncovered differential responses of OsPHDs expression to environmental stresses, including ABA (abscisic acid), water deficit, cold and high Cd. By using quantitative real-time PCR, we further confirmed the differential expression of OsPHDs under these stresses. OsPHD1/7/8/13/33 were differentially expressed under water deficit and Cd stresses, while OsPHD5/17 showed altered expression under water deficit and cold stresses. Moreover, OsPHD3/44/28 displayed differential expression under ABA and Cd stresses. In conclusion, our results provide valuable information on the rice PHD family in plant responses to environmental stress, which will be helpful for further characterizing their biological roles in responding to environmental stresses.

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“…It can identify thousands of proteins and their modifications in a single experiment [13]. It has been widely used in proteomic and phosphoproteomic analysis on Arabidopsis [14,15,16,17], maize [18,19,20,21], rice [22,23,24], soybean [25,26,27], and sorghum [28,29]. A comparative phosphoproteomic and proteomic analysis on roots of soybean seedlings identified 2692 phosphoproteins and 5509 phosphorylation sites.…”

Section: Introductionmentioning

confidence: 99%

Phosphoproteomic Analysis of Two Contrasting Maize Inbred Lines Provides Insights into the Mechanism of Salt-Stress Tolerance

Zhao

Bai

Jiang

et al. 2019

IJMS

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Salinity is a major abiotic stress that limits maize yield and quality throughout the world. We investigated phosphoproteomics differences between a salt-tolerant inbred line (Zheng58) and a salt-sensitive inbred line (Chang7-2) in response to short-term salt stress using label-free quantitation. A total of 9448 unique phosphorylation sites from 4116 phosphoproteins in roots and shoots of Zheng58 and Chang7-2 were identified. A total of 209 and 243 differentially regulated phosphoproteins (DRPPs) in response to NaCl treatment were detected in roots and shoots, respectively. Functional analysis of these DRPPs showed that they were involved in carbon metabolism, glutathione metabolism, transport, and signal transduction. Among these phosphoproteins, the expression of 6-phosphogluconate dehydrogenase 2, pyruvate dehydrogenase, phosphoenolpyruvate carboxykinase, glutamate decarboxylase, glutamate synthase, l-gulonolactone oxidase-like, potassium channel AKT1, high-affinity potassium transporter, sodium/hydrogen exchanger, and calcium/proton exchanger CAX1-like protein were significantly regulated in roots, while phosphoenolpyruvate carboxylase 1, phosphoenolpyruvate carboxykinase, sodium/hydrogen exchanger, plasma membrane intrinsic protein 2, glutathione transferases, and abscisic acid-insensitive 5-like protein were significantly regulated in shoots. Zheng58 may activate carbon metabolism, glutathione and ascorbic acid metabolism, potassium and sodium transportation, and the accumulation of glutamate to enhance its salt tolerance. Our results help to elucidate the mechanisms of salt response in maize seedlings. They also provide a basis for further study of the mechanism underlying salt response and tolerance in maize and other crops.

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A Comprehensive Proteomic Survey of ABA-Induced Protein Phosphorylation in Rice (Oryza sativa L.)

Cited by 32 publications

References 61 publications

Abscisic acid promotes jasmonic acid biosynthesis via a ‘SAPK10‐bZIP72‐AOC’ pathway to synergistically inhibit seed germination in rice (Oryza sativa)

Abscisic acid promotes jasmonic acid biosynthesis via a ‘SAPK10‐bZIP72‐AOC’ pathway to synergistically inhibit seed germination in rice (Oryza sativa)

Genome-Wide Identification of the PHD-Finger Family Genes and Their Responses to Environmental Stresses in Oryza sativa L.

Phosphoproteomic Analysis of Two Contrasting Maize Inbred Lines Provides Insights into the Mechanism of Salt-Stress Tolerance

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