A comprehensive LC–MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala

Yılmaz, Mustafa Abdullah; Ertaş, Abdülselam; Yener, İsmail; Akdeniz, Mehmet; Çakır, Oğuz; Altun, Muhammed; Demirtaş, İbrahim; Boğa, Mehmet; Temel, Hamdi

doi:10.1016/j.jpba.2018.02.059

Cited by 56 publications

(44 citation statements)

References 45 publications

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“…In the literature, the antioxidant capacities of the essential oils of Achillea species have been shown to be moderate which is in parallel with our studies . However, it is seen that various extracts (polar extracts such as those with ethanol, methanol) have high antioxidant potential …”

Section: Resultssupporting

confidence: 89%

“…In general, it can be said that the studied species have medium antimicrobial, antioxidant and enzyme activity potentials. It is known that cytotoxic activities of Achillea species are present in the literature . However, it was determined that the essential oils of the studied species had weak cytotoxic effects against MCF‐7 and HT‐29 cell lines.…”

Section: Discussionmentioning

confidence: 95%

“…[30] However, it is seen that various extracts (polar extracts such as those with ethanol, methanol) have high antioxidant potential. [31,32] Anticholinesterase enzyme activities of the studied eight species were determined according to acetyland butyrylcholinesterase enzyme inhibition methods. It was determined that none of the samples showed any activity against acetylcholinesterase enzyme.…”

Section: Results Of Biological Activitiesmentioning

confidence: 99%

“…It is known that cytotoxic activities of Achillea species are present in the literature. [31,36] However, it was determined that the essential oils of the studied species had weak cytotoxic effects against MCF-7 and HT-29 cell lines. Also in this study, the toxic effects of eight essential oils on healthy cell lines were identified and it was determined that all species studied had moderate toxic effects.…”

Section: Discussionmentioning

confidence: 98%

“…The details of the analyses related to the chemical contents of the essential oils were given in the Supporting Information . The composition of essential oils obtained by hydrodistillation method with the help of Clevenger apparatus was determined by GC/MS and GC/FID techniques …”

Section: Methodsmentioning

confidence: 99%

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A Detailed Biological and Chemical Investigation of Sixteen Achillea Species’ Essential Oils via Chemometric Approach

Yener

Yılmaz

Ölmez

et al. 2020

Chemistry & Biodiversity

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Representatives of the Achillea genus are widely used as foods or nutraceuticals. Considering the increasing demand for herbal dietary supplements with health promoting effects, the objective of this research was to evaluate the chemical composition and biological activities of the essential oils obtained from sixteen Achillea species (A. biebersteinii, A. wilhelmsii subsp. wilhelmsii, A. aleppica subsp. zederbaueri, A. vermicularis, A. monocephala, A. nobilis, A. goniocephala, A. sintenisii, A. coarctata, A. kotschyi subsp. kotschyi, A. millefolium subsp. millefolium, A. lycaonica, A. spinulifolia, A. teretifolia, A. setacea, and A. schischkinii). Anticholinesterase, antiurease, antityrosinase enzymes inhibition, antioxidant, antimicrobial, toxic and cytotoxic activities of obtained essential oils were investigated. DPPH activities were found to be very low in all studied samples, while ABTS and CUPRAC antioxidant activities were found to be moderate. In addition, all samples were found to have moderate anticholinesterase and antimicrobial effects. It has been determined that the studied species have low cytotoxicity and high toxicity. Besides, chemical composition of the essential oils were determined by GC/MS and the results were chemometrically analyzed. The chemometric analyses of Achillea species collected from nine different regions were accomplished by principal component analysis (PCA) and hierarchical cluster analysis (HCA) techniques. According to the PCA analysis, A. nobilis subsp. neilreichii was found to be different from all studied species in terms of essential oil composition. The major components found in these species were piperitone, camphor, α‐terpinene, eucalyptol, artemisia ketone, endo‐borneol, β‐eudesmol and verbenol. The fact that camphor was toxic and found in majority of the studied species stands out as a remarkable result.

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Section: Resultssupporting

confidence: 89%

Section: Discussionmentioning

confidence: 95%

Section: Results Of Biological Activitiesmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

A Detailed Biological and Chemical Investigation of Sixteen Achillea Species’ Essential Oils via Chemometric Approach

Yener

Yılmaz

Ölmez

et al. 2020

Chemistry & Biodiversity

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Detailed Phytochemical Evaluation of a Locally Endemic Species (Campanula baskilensis) by LC‐MS/MS and Its In‐Depth Antioxidant and Enzyme Inhibitory Activities

Yilmaz,

Cakir,

Izol

et al. 2023

Chemistry & Biodiversity

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The importance of bioactive plant species in the scientific world is increasing day by day. The relationship between health and traditional‐modern life, promotes the creation of new value‐added natural products. This is the first research to conduct a bioactivity and chemical composition analysis of Campanula baskilensis species, which belongs to the medicinally important genus Campanula L (Campanulaceae). The aim of the current study is to quantitatively investigate the phytochemical contents of aerial and root parts of different C. baskilensis extracts (ethanol, methanol, and water) by LC‐MS/MS and to evaluate their total phenolic and flavonoid contents, antioxidant and enzyme inhibitory activities. Remarkably, LC‐MS/MS results revealed that, high amounts of quinic acid (53.6 mg/g aerial‐MeOH extract), fumaric acid (6.3 mg/g aerial‐H2O extract, 2.5 mg/g root‐ H2O extract), protocatechuic acid (11.4 mg/g aerial‐H2O extract), vanilic acid (1.4 mg/g aerial‐EtOH extract), quercetin‐3‐O‐rutinoside (rutin) (2.3 mg/g aerial‐EtOH extract), hesperetin 7‐rutinoside (hesperidin) (2.0 mg/g aerial‐EtOH extract), kaempferol‐3‐O‐rutinoside (nicotiflorin) (5.5 mg/g aerial‐EtOH extract) were detected in the extracts of the species. Considering the bioactivity tests performed on C. baskilensis extracts, aerial‐H2O extract showed significant activity in all antioxidant assays. However, ethanol extracts of root and aerial parts exhibited the highest activities in all enzyme inhibitory tests.

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Integration of mass spectral fingerprinting analysis with precursor ion (MS1) quantification for the characterisation of botanical extracts: application to extracts of Centella asiatica (L.) Urban

Magaña

Wright

Vaswani

et al. 2020

Phytochemical Analysis

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Introduction: The phytochemical composition of plant material governs the bioactivity and potential health benefits as well as the outcomes and reproducibility of laboratory studies and clinical trials. Objective: The objective of this work was to develop an efficient method for the indepth characterisation of plant extracts and quantification of marker compounds that can be potentially used for subsequent product integrity studies. Centella asiatica (L.) Urb., an Ayurvedic herb with potential applications in enhancing mental health and cognitive function, was used as a case study. Methods: A quadrupole time-of-flight analyser in conjunction with an optimised high-performance liquid chromatography (HPLC) separation was used for in-depth untargeted fingerprinting and post-acquisition precursor ion quantification to determine levels of distinct phytochemicals in various C. asiatica extracts. Results: We demonstrate the utility of this workflow for the characterisation of extracts of C. asiatica. This integrated workflow allowed the identification or tentative identification of 117 compounds, chemically interconnected based on Tanimoto chemical similarity, and the accurate quantification of 24 phytochemicals commonly found in C. asiatica extracts. Conclusion: We report a phytochemical analysis method combining liquid chromatography, high resolution mass spectral data acquisition, and post-acquisition interrogation that allows chemical fingerprints of botanicals to be obtained in conjunction with accurate quantification of distinct phytochemicals. The variability in the composition of specialised metabolites across different C. asiatica accessions was substantial, demonstrating that detailed characterisation of plant extracts is a

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A comprehensive LC–MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala

Cited by 56 publications

References 45 publications

A Detailed Biological and Chemical Investigation of Sixteen Achillea Species’ Essential Oils via Chemometric Approach

A Detailed Biological and Chemical Investigation of Sixteen Achillea Species’ Essential Oils via Chemometric Approach

Detailed Phytochemical Evaluation of a Locally Endemic Species (Campanula baskilensis) by LC‐MS/MS and Its In‐Depth Antioxidant and Enzyme Inhibitory Activities

Integration of mass spectral fingerprinting analysis with precursor ion (MS1) quantification for the characterisation of botanical extracts: application to extracts of Centella asiatica (L.) Urban

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