A comprehensive insight from microalgae production process to characterization of biofuel for the sustainable energy

Kowthaman, C.N.; Kumar, P. Senthil; Selvan, V. Arul Mozhi; Duraisamy, Ganesh

doi:10.1016/j.fuel.2021.122320

Cited by 44 publications

(17 citation statements)

References 149 publications

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“…The copyright holder for this preprint this version posted May 5, 2022. ; https://doi.org/10.1101/2022.05.05.490790 doi: bioRxiv preprint needed to screen competitive strains (for example, lipid-rich and fast-growing strains), and to improve large-scale cultivation based on understanding the effects of various culture conditions, such as light intensity, nutrition, carbon dioxide, etc [6,7]. Usually, these works are accomplished by using traditional flask-type algae culturing and lipid analyzing technique [8,9].…”

Section: Introductionmentioning

confidence: 99%

A microfluidic droplet array demonstrating high-throughput screening in individual lipid-producing microalgae

Zheng

Cui

et al. 2022

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

A microfluidic droplet array demonstrating high-throughput screening in individual lipid-producing microalgae

Zheng

Cui

et al. 2022

Analytica Chimica Acta

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“…They could convert the green house gas and sun light into chemical energy through their conventional physiological pathway, part of which is what we used as biodiesel (Fig.1a). Many algal strains are able to accumulate in certain conditions, high amounts of fatty acids in up to 20-50% of their dry weight [12]. Additionally, microalgae have faster growth rate, less freshwater demand for cultivation, no or minimal competition with food supply, land usage and associated environmental impacts since microalgae could live in very harsh environment, such as saline/brackish water/coastal seawater on non-arable land [6-8].…”

Section: Introductionmentioning

confidence: 99%

“…The principal contradiction is that the production cost of current algae derived biofuels and bioproducts remains well above economic viability. Significant improvements are still required throughout all steps of the microalgae-based biofuel/bioproduct production pipeline [6, 7, 12].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic chemostatic bioreactor for high-throughput screening and sustainable co-harvesting of biomass and biodiesel in microalgae

Zheng

Cui

Lü

et al. 2022

Preprint

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As a renewable and sustainable source for energy, environment, and biomedical applications, microalgae and microalgal biodiesel have attracted great attention. However, their applications are confined due to the cost-efficiency of microalgal mass production. One-step strategy and continuous culturing systems could be solutions. However, current studies for optimization throughout microalgae-based biofuel production pipelines are generally derived from the batch culture process. Better tools are needed to study algal growth kinetics in continuous systems. A microfluidics chemostatic bioreactor array was presented, providing low-adhesion cultivation for algae in the gas, nutrition, and temperature (GNT) well-controlled environment with high throughput. The chip wasused to mimic the continuous culture environment of bioreactors. It allowed simultaneously studying of 8×8 different chemostatic conditions on algal growth and oil production in parallel on a 7×7 cm2 footprint. On-chip experiments of batch and continuous cultures of Chlorella. sp. were performed to study growth and lipid accumulation under different nitrogen concentrations. The results demonstrated that microalgal cultures can be regulated to grow and accumulate lipids concurrently, thus enhancing lipid productivity in one step. The developed on-chip culturing condition screening, which was more suitable for continuous bioreactor, was achieved at a half shorter time, 64-times higher throughput, and less reagent consumption. It could be used to establish chemostat cultures in continuous bioreactors which can dramatically accelerate the development of renewable and sustainable algal for CO2 fixation and biosynthesis and related systems for advanced sustainable energy, food, pharmacy, and agriculture with enormous social and ecological benefits.TEASERSustainable microfluidic bioreactor for 64 times higher-throughput screening CO2 fixation and biomass and biodiesel production in microalgae.

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“…However, the high cost of biofuels limits their availability of commercialized production [5]. It is urgently needed to screen competitive strains (for example, lipid-rich and fast-growing strains), and to improve large-scale cultivation based on understanding the effects of various culture conditions, such as light intensity, nutrition, carbon dioxide, etc [6, 7]. Usually, these works are accomplished by using traditional flask-type algae culturing and lipid analyzing technique [8, 9].…”

Section: Introductionmentioning

confidence: 99%

“…Lipid productivity is determined by the growth rate and lipid production of microalgae [5]. Identification and isolation of strains with such high performance is critical but challenging, especially in a high-throughput manner [7, 9]. In traditional library screening, cell populations are diluted and plated on media plates.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic droplet array demonstrating high-throughput screening in individual lipid-producing microalgae

Zheng¹,

Gu²,

Cui³

et al. 2022

Preprint

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Microalgae are a group of photoautotrophic microorganisms which could use carbon dioxide for autosynthesis. They have been envisioned as one of the most prospective feedstock for renewable oil. However, great endeavors will still be needed to increase their economic feasibility; the screening of competitive species and suitable culture conditions are such issues. To greatly accelerate these rather laborious steps and also improve their experimental lump-sum-manner, we developed a microfluidic droplet-based 2000 resolution "identification card", which allowed high throughput real-time monitoring of individual algae among population. A novel fluid-blocking-based droplet generating and trapping performance were integrated in the platform which made it excellent in operational simplicity, rapidity and stability and full of the potentials in single-cell-isolation/screening. The developed platform was successfully used to screen three unicellular algae, namely, Isochrysis zhanjiangensis, Platymonas subcordiformis and Platymonas helgolandica var. tsingtaoensis. In situ bioassays of the lipid accumulation and cell proliferation at single cell level for interspecies comparison were possible. Nitrogen stress condition can be indentified that induce positive-skewed frequency distribution of lipid content.

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A comprehensive insight from microalgae production process to characterization of biofuel for the sustainable energy

Cited by 44 publications

References 149 publications

A microfluidic droplet array demonstrating high-throughput screening in individual lipid-producing microalgae

A microfluidic droplet array demonstrating high-throughput screening in individual lipid-producing microalgae

Microfluidic chemostatic bioreactor for high-throughput screening and sustainable co-harvesting of biomass and biodiesel in microalgae

A microfluidic droplet array demonstrating high-throughput screening in individual lipid-producing microalgae

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