A Comprehensive Human Linkage Map with Centimorgan Density

Murray, Jeffrey C.; Buetow, Kenneth H.; Weber, James L.; Ludwigsen, Susan; Scherpbier-Heddema, T; Manion, Frank J.; Quillen, J; Sheffield, Val C.; Sunden, Sara L.F.; Duyk, Geoffrey M.

doi:10.1126/science.8091227

Cited by 479 publications

(233 citation statements)

References 56 publications

(34 reference statements)

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“…Indeed, the first large scale effort to produce a human genetic map, was performed mainly using RFLP markers, the best known genetic markers at the time [20]. However, with the generalisation of PCR and the demonstration of Mendelian inheritance of the multiple alleles due to variations in the number of short nucleotide repeats observed at microsatellite loci [50,81], a change in strategy was quickly made and all the successive genetic maps in humans [14,18,82] were based mainly on this new type of marker. Two main reasons were behind this quick shift.…”

Section: Introduction: Older Types Of Molecular Genetic Markersmentioning

confidence: 99%

A review on SNP and other types of molecular markers and their use in animal genetics

et al. 2002

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-During the last ten years, the use of molecular markers, revealing polymorphism at the DNA level, has been playing an increasing part in animal genetics studies. Amongst others, the microsatellite DNA marker has been the most widely used, due to its easy use by simple PCR, followed by a denaturing gel electrophoresis for allele size determination, and to the high degree of information provided by its large number of alleles per locus. Despite this, a new marker type, named SNP, for Single Nucleotide Polymorphism, is now on the scene and has gained high popularity, even though it is only a bi-allelic type of marker. In this review, we will discuss the reasons for this apparent step backwards, and the pertinence of the use of SNPs in animal genetics, in comparison with other marker types.SNP / microsatellite / molecular marker / genome / polymorphism

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Section: Introduction: Older Types Of Molecular Genetic Markersmentioning

confidence: 99%

A review on SNP and other types of molecular markers and their use in animal genetics

et al. 2002

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“…LOH, FISH, and CGH were performed as previously described (Cliby et al, 1993;Ritland et al, 1995;Qian et al, 1995;Mohapatra et al, 1995Mohapatra et al, , 1998Piper et al, 1995). Selected microsatellite markers were placed in their most probable linkage order based upon radiation hybrid and physical mapping (GDB Human Genome Database, 1990;Collins et al, 1992;Murray et al, 1994;Ashworth et al, 1995). Allelic dosage for LOH analyses was determined by visual inspection and by multiplex PCR using a control primer set to normalize for DNA loading.…”

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confidence: 99%

Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype

et al. 1999

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Allelic alterations of chromosomes 1 and 19 are frequent events in human di use gliomas and have recently proven to be strong predictors of chemotherapeutic response and prolonged survival in oligodendrogliomas (Cairncross et al., 1998; Smith et al., submitted). Using 115 human di use gliomas, we localized regions of common allelic loss on chromosomes 1 and 19 and assessed the association of these deletion intervals with glioma histological subtypes. Further, we evaluated the capacity of multiple modalities to detect these alterations, including loss of heterozygosity (LOH),¯uorescence in situ hybridization (FISH), and comparative genomic hybridization (CGH). The correlation coe cients for detection of 1p and 19q alterations, respectively, between modalities were: 0.98 and 0.87 for LOH and FISH, 0.79 and 0.60 for LOH and CGH, and 0.79 and 0.53 for FISH and CGH. Minimal deletion regions were de®ned on 19q13.3 (D19S412-D19S596) and 1p (D1S468-D1S1612). Loss of the 1p36 region was found in 18% of astrocytomas (10/55) and in 73% (24/33) of oligodendrogliomas (P50.0001), and loss of the 19q13.3 region was found in 38% (21/55) of astrocytomas and 73% (24/33) of oligodendrogliomas (P=0.0017). Loss of both regions was found in 11% (6/55) of astrocytomas and in 64% (21/33) of oligodendrogliomas (P50.0001). All gliomas with LOH on either 1p or 19q demonstrated loss of the corresponding FISH probe, 1p36 or 19q13.3, suggesting not only locations of putative tumor suppressor genes, but also a simple assay for assessment of 1p and 19q alterations as diagnostic and prognostic markers.

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“…The contigs were positioned using markers that were also present in the available genetic (Murray et al 1994;Dib et al 1996) or radiation hybrid (RH) maps of the human genome. The average STS spacing of these combined maps is ∼1/250 kb for the whole genome maps Hudson et al 1995), and up to 1/70 kb for selected single chromosomes (Chumakov et al 1992;Foote et al 1992;Collins et al 1995;Doggett et al 1995;Gemmill et al 1995;Krauter et al 1995;Bouffard et al 1997;Nagaraja et al 1997).…”

Section: Determining the Human Genome Sequencementioning

confidence: 99%

Toward a Complete Human Genome Sequence

Centre¹,

Cente²

1998

Genome Res.

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We have begun a joint program as part of a coordinated international effort to determine a complete human genome sequence. Our strategy is to map large-insert bacterial clones and to sequence each clone by a random shotgun approach followed by directed finishing. As of September 1998, we have identified the map positions of bacterial clones covering ∼860 Mb for sequencing and completed >98 Mb (∼3.3%) of the human genome sequence. Our progress and sequencing data can be accessed via the World Wide Web (http://webace.sanger.ac.uk/HGP/ orhttp://genome.wustl.edu/gsc/).

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A Comprehensive Human Linkage Map with Centimorgan Density

Cited by 479 publications

References 56 publications

A review on SNP and other types of molecular markers and their use in animal genetics

A review on SNP and other types of molecular markers and their use in animal genetics

Localization of common deletion regions on 1p and 19q in human gliomas and their association with histological subtype

Toward a Complete Human Genome Sequence

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