A Comprehensive Comparison of Rapid RNA Extraction Methods for Detection of SARS-CoV-2 as the Infectious Agent of the Upper Respiratory Tract using Direct RT-LAMP Assay

Moazami Goodarzi, Maryam; Taghizadeh Pirposhteh, Razieh; Ravan, Hadi; Vahidian, Farnaz; Kheirkhah, Omolbani; Fotouhi Ardakani, Reza; Fotouhi, Fatemeh

doi:10.4103/abr.abr_63_23

Advanced Biomedical Research

2023

DOI: 10.4103/abr.abr_63_23

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A Comprehensive Comparison of Rapid RNA Extraction Methods for Detection of SARS-CoV-2 as the Infectious Agent of the Upper Respiratory Tract using Direct RT-LAMP Assay

Maryam Moazami Goodarzi,

Razieh Taghizadeh Pirposhteh,

Hadi Ravan

et al.

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2024

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Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review

Goodarzi,

Jalalirad

2024

Biotech & Bioengineering

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Increasing attention has been paid to the purity of therapeutic proteins imposing extensive costs and challenges to the downstream processing of biopharmaceuticals. One of the efforts, that has been exerted to overcome such limitations, was developing multimodal or mixed‐mode chromatography (MMC) resins for launching selective, orthogonal, non‐affinity purification platforms. Despite relatively extensive usage of MMC resins, their real potential and fulfillment have not been extensively reviewed yet. In this work, the explanation of practical and key aspects of downstream processing of recombinant proteins with or without MMC resins was debated, as being useful for further purification process development. This review has been written as a step‐by‐step guide to deconvolute both inherent protein purification and MMC complexities. Here, after complete elucidation of the potential of MMC resins, the effects of frequently used additives (mobile phase modifiers) and their possible interactions during the purification process, the critical characteristics of common product‐related impurities (e.g., aggregates, charge variants, fragments), host‐related impurities (e.g., host cell protein and DNA) and process related impurities (e.g., endotoxin, and viruses) with solved or unsolved challenges of traditional and MMC resins have been discussed. Such collective experiences which are reported in this study could be considered as an applied guide for developing successful downstream processing in challenging conditions by providing a clear insight into complex MMC resins and impurities.

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Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review

Goodarzi,

Jalalirad

2024

Biotech & Bioengineering

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A Comprehensive Comparison of Rapid RNA Extraction Methods for Detection of SARS-CoV-2 as the Infectious Agent of the Upper Respiratory Tract using Direct RT-LAMP Assay

Cited by 1 publication

References 28 publications

Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review

Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review

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