A Comprehensive Analysis of the Combined Effects of High Light and High Temperature Stresses on Gene Expression in Sunflower

Abstract

Taken together, these results indicate that vegetative and reproductive tissues employ different transcriptome responses to these stress treatments. Careful examination of the putative functions of these genes revealed novel and specific responses. The potential roles of many of the differentially expressed genes in stress tolerance are mentioned and discussed.

Sensitivity of flowering plant gametophytes to temperature fluctuations

“…A recent expression profiling analysis of maturing tomato microspores subjected to a short heat shock treatment reveals an up-regulation not only of genes implicated in similar pathways, but also of some pollen specific genes that might represent pollen-specific response mechanisms (Frank et al, 2009). The effect of high temperature and high light induced different transcriptome responses between vegetative and reproductive tissues of sunflower with some specific functions in both tissues (Hewezi et al, 2008) Olea europea 20ºC, 25ºC and 30ºC constant temperatures.…”

Understanding the molecular events underpinning cultivar differences in the physiological performance and heat tolerance of cotton (Gossypium hirsutum)

“…Quantitative real-time PCR Q-PCR was used to validate the treatment and cultivar differences found using microarray analysis (Busch et al 2005;Ogawa et al 2007;Hewezi et al 2008) and for a detailed evaluation of gene expression over a short (7 h) high-temperature treatment period. Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA) was used in accordance with the manufacturer's instructions with 5mg total RNA and oligo (dT) primers to generate cDNA.…”

“…Plant responses to high temperature stress at a molecular level have been studied predominantly in model systems such as Arabidopsis thaliana (L.), although some crop species with a higher inherent heat tolerance such as sunflower (Helianthus annuus L.), potato (Solanum tuberosum L.) and tobacco (Nicotiana tabacum L.) have also been studied (Rizhsky et al 2002;Rensink et al 2005;Hewezi et al 2008) and show similar responses to abiotic stress as model species (Rizhsky et al 2004). Molecular analysis indicates that the heat response is complex and varied, involving significant changes in global gene expression (Rizhsky et al 2004;Busch et al 2005;Rensink et al 2005;Kant et al 2008;Larkindale and Vierling 2008) and multigene interactions (Humphreys and Humphreys 2005).…”

“…Initial induction of genes encoding heat stress transcription factors and HSPs is accompanied by alteration of genes associated with protein biosynthesis, folding and processing (Busch et al 2005;Hewezi et al 2008;Larkindale and Vierling 2008;Yokotani et al 2008) and protein degradation (Rizhsky et al 2002;Hewezi et al 2008;Kant et al 2008;Tian et al 2009). Increases in respiration are associated with the upregulation of genes encoding proteins involved in the catabolism of assimilates and glycolysis (Rizhsky et al 2002;Kant et al 2008;Larkindale and Vierling 2008).…”

“…Increases in respiration are associated with the upregulation of genes encoding proteins involved in the catabolism of assimilates and glycolysis (Rizhsky et al 2002;Kant et al 2008;Larkindale and Vierling 2008). Despite a potential decrease in energy availability, genes associated with transport (Busch et al 2005;Hewezi et al 2008), signalling (Busch et al 2005) and overall metabolism (Busch et al 2005;Hewezi et al 2008), specifically nitrogen and sulfur (Kant et al 2008), are induced under heat stress.…”