A comprehensive analysis of neurotrophins and neurotrophin tyrosine kinase receptors expression during development of zebrafish

Nittoli, Valeria; Sepe, Rosa Maria; Coppola, Ugo; D’Agostino, Ylenia; Felice, Elena De; Palladino, Antonio; Vassalli, Quirino Attilio; Locascio, Annamaria; Ristoratore, Filomena; Spagnuolo, Antonietta; D’Aniello, Salvatore; Sordino, Paolo

doi:10.1002/cne.24391

Cited by 30 publications

(45 citation statements)

References 107 publications

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“…In this study we investigate the expression of ntrk2b because it is the most expressed isoform in mechanosensory systems, if compared to ntrk2a [13,53]. In the last decades, several animal models, including zebrafish, have been used in biomedicine in order to model sensorineural hearing loss and study the morpho-functional alteration and ototoxic lesions due to pharmacological treatments mainly carried out with aminoglycoside antibiotics in the sensory cells of the inner ear [37,38]. It is well known that the spontaneous regeneration of hair cells is not possible in mammals whereas fish maintain the capacity to regenerate damaged hair cells within a few days [25,39,40].…”

Section: Discussionmentioning

confidence: 99%

Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear

Germanà¹,

Guerrera²,

Laurà³

et al. 2020

IJMS

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Brain-derived neurotrophic factor (BDNF), a member of the neurotrophin family, is involved in multiple and fundamental functions of the central and peripheral nervous systems including sensory organs. Despite recent advances in knowledge on the functional significance of BDNF and TrkB in the regulation of the acoustic system of mammals, the localization of BDNF/TrkB system in the inner ear of zebrafish during development, is not well known. Therefore, the goal of the present study is to analyze the age-dependent changes using RT-PCR, Western Blot and single and double immunofluorescence of the BDNF and its specific receptor in the zebrafish inner ear. The results showed the mRNA expression and the cell localization of BDNF and TrkB in the hair cells of the crista ampullaris and in the neuroepithelium of the utricle, saccule and macula lagena, analyzed at different ages. Our results demonstrate that the BDNF/TrkB system is present in the sensory cells of the inner ear, during whole life. Therefore, this system might play a key role in the development and maintenance of the hair cells in adults, suggesting that the zebrafish inner ear represents an interesting model to study the involvement of the neurotrophins in the biology of sensory cells

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Section: Discussionmentioning

confidence: 99%

Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear

Germanà¹,

Guerrera²,

Laurà³

et al. 2020

IJMS

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“…The oncogenic activation of NTRK can occur in several ways, including structural chromosomal rearrangements leading to gene fusions, splice variants, mutations, copy number alterations and In the peripheral nervous system (PNS), neutrophins binding and NTRK signaling is required for the survival of sensory and sympathetic ganglia [52][53][54][55][56]. Loss of NTRK expression in the development phase of mice and zebrafish has several consequences on their sensory systems, such as gustatory deficits and hearing and vision impairment [57,58]. Within CNS, NTRK gene expression is fundamental for neuron migration to the cortical layer (in particular for cerebellar granule neurons), and for their growth and maturation.…”

Section: The Oncogenic Role Of Ntrk: Fusions Versus Other Alterationsmentioning

confidence: 99%

NTRK Fusions in Central Nervous System Tumors: A Rare, but Worthy Target

Gambella

Senetta

Collemi

et al. 2020

IJMS

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The neurotrophic tropomyosin receptor kinase (NTRK) genes (NTRK1, NTRK2, and NTRK3) code for three transmembrane high-affinity tyrosine-kinase receptors for nerve growth factors (TRK-A, TRK-B, and TRK-C) which are mainly involved in nervous system development. Loss of function alterations in these genes can lead to nervous system development problems; conversely, activating alterations harbor oncogenic potential, promoting cell proliferation/survival and tumorigenesis. Chromosomal rearrangements are the most clinically relevant alterations of pathological NTRK activation, leading to constitutionally active chimeric receptors. NTRK fusions have been detected with extremely variable frequencies in many pediatric and adult cancer types, including central nervous system (CNS) tumors. These alterations can be detected by different laboratory assays (e.g., immunohistochemistry, FISH, sequencing), but each of these approaches has specific advantages and limitations which must be taken into account for an appropriate use in diagnostics or research. Moreover, therapeutic targeting of this molecular marker recently showed extreme efficacy. Considering the overall lack of effective treatments for brain neoplasms, it is expected that detection of NTRK fusions will soon become a mainstay in the diagnostic assessment of CNS tumors, and thus in-depth knowledge regarding this topic is warranted.

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“…As a bonus, zebrafish produces a large number of externally fertilized nearly transparent eggs which exhibit highly synchronous and rapid development. Aside from this, the expression of neurotrophins and their tyrosine kinase receptor genes have been well‐documented in the developing zebrafish (De Felice et al, ; Nittoli et al, ).…”

Section: Bdnf and Brain Developmentmentioning

confidence: 99%

Neuroanatomical distribution and functions of brain‐derived neurotrophic factor in zebrafish (Danio rerio) brain

Anand

Mondal

2019

J of Neuroscience Research

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Brain‐derived neurotrophic factor (BDNF) is an extensively studied protein that is evolutionarily conserved and widely distributed in the brain of vertebrates. It acts via its cognate receptors TrkB and p75NTR and plays a central role in the developmental neurogenesis, neuronal survival, proliferation, differentiation, synaptic plasticity, learning and memory, adult hippocampal neurogenesis, and brain regeneration. BDNF has also been implicated in a plethora of neurological disorders. Hence, understanding the processes that are controlled by BDNF and their regulating mechanisms is important. Although, BDNF has been thoroughly studied in the mammalian models, contradictory effects of its functions have been reported on several occasions. These contradictory effects may be attributed to the sheer complexity of the mammalian brain. The study of BDNF and its associated functions in a simpler vertebrate model may provide some clarity about the effects of BDNF on the neurophysiology of the brain. Keeping that in mind, this review aims at summarizing the current knowledge about the distribution of BDNF and its associated functions in the zebrafish brain. The main focus of the review is to give a comparative overview of BDNF distribution and function in zebrafish and mammals with respect to distinct life stages. We have also reviewed the regulation of bdnf gene in zebrafish and discussed its role in developmental and adult neurogenesis.

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A comprehensive analysis of neurotrophins and neurotrophin tyrosine kinase receptors expression during development of zebrafish

Cited by 30 publications

References 107 publications

Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear

Expression and Localization of BDNF/TrkB System in the Zebrafish Inner Ear

NTRK Fusions in Central Nervous System Tumors: A Rare, but Worthy Target

Neuroanatomical distribution and functions of brain‐derived neurotrophic factor in zebrafish (Danio rerio) brain

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