A Comprehensive Analysis of MicroRNA Expression During Human Keratinocyte Differentiation In Vitro and In Vivo

Hildebrand, Janosch; Rütze, Martin; Walz, Nicole; Gallinat, Stefan; Wenck, Horst; Deppert, Wolfgang; Grundhoff, Adam; Knott, Anja

doi:10.1038/jid.2010.268

Cited by 98 publications

(98 citation statements)

References 24 publications

(36 reference statements)

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“…21,[25][26][27] In human skin epidermis, multiple microRNAs are thought to regulate gene expression of keratins during keratinocyte differentiation in vitro and in vivo. 28 Therefore, it is speculated that microRNA-induced repression of the K13 gene translation might occur as posttranscriptional regulation in the oral keratinocytes.…”

Section: Discussionmentioning

confidence: 99%

Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

et al. 2012

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Immunohistochemical loss of keratin (K)13 is one of the most valuable diagnostic criteria for discriminating carcinoma in situ (CIS) from non-malignancies in the oral mucosa while K13 is stably immunolocalized in the prickle cells of normal oral epithelium. To elucidate the molecular mechanism for the loss of K13, we compared the immunohistochemical profiles for K13 and K16 which is not expressed in normal epithelia, but instead enhanced in CIS, with their mRNA levels by in-situ hybridization in formalin-fixed paraffin sections prepared from 23 CIS cases of the tongue, which were surgically removed. Reverse transcriptase-PCR was also performed using RNA samples extracted from laser-microdissected epithelial fragments of the serial paraffin sections in seven of the cases. Although more enhanced expression levels for K16 were confirmed at both the protein and gene levels in CIS in these seven cases, the loss of K13 was associated with repressed mRNA levels in four cases, but not in the other three cases. The results suggest that the loss of K13 is partly due to its gene repression, but may also be due to some unknown post-translational events.

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Section: Discussionmentioning

confidence: 99%

Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

et al. 2012

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“…A functional relationship between keratinocyte miRNAs and psoriasis was reported, and it was determined that miR-125b modulates abnormal keratinocyte proliferation in psoriasis by targeting FGFR2 (16,17). The miRNA expression pattern during human keratinocyte differentiation has also been investigated (18). One recent study analyzed UVB-dependent miRNA expression profile changes in keratinocytes (19).…”

Section: Introductionmentioning

confidence: 99%

Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes

Choe

et al. 2012

International Journal of Molecular Medicine

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Abstract. This study aimed to evaluate the protective effects of Centella asiatica (C. asiatica) against ultraviolet B (UVB) damage in human keratinocytes using microRNA (miRNA) expression profiling analysis. Titrated extract of C. asiatica (TECA) demonstrated low cytotoxicity in normal human HaCaT keratinocytes only at low doses (<5 µg/ml). UVB (50 mJ/cm 2 ) irradiation significantly decreased cell viability, and TECA treatment decreased the UVB toxicity. By using miRNA microarrays, we determined that 72 miRNAs had an altered expression following TECA treatment in UVB-irradiated keratinocytes (46 upregulated and 26 downregulated). Using an miRNA target gene prediction tool and Gene Ontology (GO) analysis, we determined that miRNAs with altered expression were functionally related with the inhibition of apoptosis and cell proliferation. Overall, these results provide meaningful information to facilitate the understanding of TECA-mediated UVB protection in human keratinocytes.

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“…In vitro studies suggest functions for miRNAs in epidermal keratinocyte differentiation (Hildebrand et al, 2011;Xu et al, 2011). miR-205 is highly expressed in epithelia and promotes keratinocyte migration in vitro (Ryan et al, 2006;Yu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Inducible deletion of epidermal Dicer and Drosha reveals multiple functions for miRNAs in postnatal skin

et al. 2012

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SUMMARYMicroRNAs (miRNAs) regulate the expression of many mammalian genes and play key roles in embryonic hair follicle development; however, little is known of their functions in postnatal hair growth. We compared the effects of deleting the essential miRNA biogenesis enzymes Drosha and Dicer in mouse skin epithelial cells at successive postnatal time points. Deletion of either Drosha or Dicer during an established growth phase (anagen) caused failure of hair follicles to enter a normal catagen regression phase, eventual follicular degradation and stem cell loss. Deletion of Drosha or Dicer in resting phase follicles did not affect follicular structure or epithelial stem cell maintenance, and stimulation of anagen by hair plucking caused follicular proliferation and formation of a primitive transient amplifying matrix population. However, mutant matrix cells exhibited apoptosis and DNA damage and hair follicles rapidly degraded. Hair follicle defects at early time points post-deletion occurred in the absence of inflammation, but a dermal inflammatory response and hyperproliferation of interfollicular epidermis accompanied subsequent hair follicle degradation. These data reveal multiple functions for Drosha and Dicer in suppressing DNA damage in rapidly proliferating follicular matrix cells, facilitating catagen and maintaining follicular structures and their associated stem cells. Although Drosha and Dicer each possess independent non-miRNA-related functions, the similarity in phenotypes of the inducible epidermal Drosha and Dicer mutants indicates that these defects result primarily from failure of miRNA processing. Consistent with this, Dicer deletion resulted in the upregulation of multiple direct targets of the highly expressed epithelial miRNA miR-205.

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A Comprehensive Analysis of MicroRNA Expression During Human Keratinocyte Differentiation In Vitro and In Vivo

Cited by 98 publications

References 24 publications

Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

Loss of keratin 13 in oral carcinoma in situ: a comparative study of protein and gene expression levels using paraffin sections

Centella asiatica protects against UVB-induced HaCaT keratinocyte damage through microRNA expression changes

Inducible deletion of epidermal Dicer and Drosha reveals multiple functions for miRNAs in postnatal skin

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