A Composite Motif of the Drosophila Morphogenetic Protein Bicoid Critical to Transcription Control

Zhao, Chen; Fu, Dianzheng; Davé, Vrushank; Ma, Jun

doi:10.1074/jbc.m302714200

Cited by 17 publications

(31 citation statements)

References 44 publications

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“…Our experiments show that overexpression of dCBP can partially restore the activity of Bcd(A52-56) on the hb-CAT reporter in S2 cells. 2 This derivative is normally inactive on this reporter due to a strengthened self-inhibitory func- tion (20,27). These findings further highlight the importance of a well controlled balance between HAT and HDAC activities in regulating transcription.…”

Section: Fig 2 Bcd and Dcbp Interact With Each Othermentioning

confidence: 68%

“…Our previous experiments have shown that increasing the spacing between the symmetric pairs of Bcd binding sites can alter the Bcd protein domains required for cooperative DNA binding and the sensitivity to a self-inhibitory function of the protein (20). These experiments suggested that enhancer architecture can influence how Bcd utilizes its domains for different functions (20,41).…”

Section: Fig 2 Bcd and Dcbp Interact With Each Othermentioning

confidence: 99%

“…The HindIII-XbaI fragments were then isolated and cloned into the expression vector pFY442 (26). The GST-Bcd fusion plasmid pDF364 was generated by cloning appropriate PCR product into the EcoRI-XbaI sites of the pGEX-KG-based vector pAY564 (27). The plasmids for in vitro synthesis of dCBP fragments (pDF370, pDF371, and pDF373) were generated by cloning the appropriate PCR products into the NdeI-XbaI sites of pFY441 (26).…”

Section: Methodsmentioning

confidence: 99%

“…Since CBP and TFIIB can interact with each other (51), it is possible that dCBP can also participate in the direct recruitment of GTFs such as TFIIB. One of our major motivations for performing this current study was our earlier finding that the activity of Bcd is inhibited by co-repressor complexes such as Sin3A-HDAC (27,52). We reasoned that the inhibitory function of the HDAC complexes most likely would counteract the positive role of a HAT co-activator activity (or activities).…”

Section: Fig 2 Bcd and Dcbp Interact With Each Othermentioning

confidence: 99%

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The Co-activator CREB-binding Protein Participates in Enhancer-dependent Activities of Bicoid

Wen

2004

Journal of Biological Chemistry

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Bicoid (Bcd) is a transcriptional activator required for early embryonic patterning in Drosophila. Despite extensive studies, it currently remains unclear how Bcd activates transcription and what proteins participate in its activation process. In this report, we describe experiments to analyze the role of the Drosophila co-activator dCBP in Bcd-mediated activation. In Drosophila S2 cells, the Bcd activity is increased by the co-transfection of plasmids expressing dCBP and reduced by doublestranded RNA-mediated interference against dCBP. We further show that Bcd and dCBP can interact with each other and that Bcd-interacting domains of dCBP can cause dominant negative effects on Bcd activity in S2 cells. Our comparison of two Bcd-responsive enhancers, hunchback (hb) and knirps (kni), reveals a differential role of dCBP in facilitating Bcd activation. A dCBP mutant defective in its histone acetyltransferase activity exhibits a reduced, but not abolished, co-activator function for Bcd. Our chromatin immunoprecipitation experiments show that dCBP can increase not only the occupancy of Bcd itself at the enhancers but also the recruitment of general transcription factors to the promoter. Together, these experiments suggest that dCBP is an enhancer-dependent co-activator of Bcd, facilitating its activation through multiple mechanisms.

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Section: Fig 2 Bcd and Dcbp Interact With Each Othermentioning

confidence: 68%

Section: Fig 2 Bcd and Dcbp Interact With Each Othermentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Fig 2 Bcd and Dcbp Interact With Each Othermentioning

confidence: 99%

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The Co-activator CREB-binding Protein Participates in Enhancer-dependent Activities of Bicoid

Wen

2004

Journal of Biological Chemistry

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“…SAP18 was purified as part of a mammalian SIN3 complex (28). It has also been shown to interact with Drosophila bicoid, which interacts with the PAH3 and -4 domains of SIN3 (37,52). Based on these results, Singh et al (37) hypothesized that SAP18 would be a component of the Drosophila SIN3 complex.…”

Section: Sin3 Isoforms Interact With a Common Set Of Proteins-mentioning

confidence: 99%

Drosophila SIN3 Isoforms Interact with Distinct Proteins and Have Unique Biological Functions

Spain

Caruso

Swaminathan

et al. 2010

Journal of Biological Chemistry

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The SIN3 corepressor serves as a scaffold for the assembly of histone deacetylase (HDAC) complexes. SIN3 and its associated HDAC have been shown to have critical roles in both development and the regulation of cell cycle progression. Although multiple SIN3 isoforms have been reported in simple to complex eukaryotic organisms, the mechanisms by which such isoforms regulate specific biological processes are still largely uncharacterized. To gain insight into how SIN3 isoform-specific function contributes to the growth and development of a metazoan organism, we have affinity-purified two SIN3 isoform-specific complexes, SIN3 187 and 220, from Drosophila S2 cells and embryos. We have identified a number of proteins common to the complexes, including the HDAC RPD3, as well as orthologs of several proteins known to have roles in regulating cell proliferation in other organisms. We additionally identified factors, including the histone demethylase little imaginal discs and histoneinteracting protein p55, that exhibited a preferential interaction with the largest SIN3 isoform. Our experiments indicate that the isoforms are associated with distinct HDAC activity and are recruited to unique and shared sites along polytene chromosome arms. Furthermore, although expression of SIN3 220 can substitute for genetic loss of other isoforms, expression of SIN3 187 does not support Drosophila viability. Together our findings suggest that SIN3 isoforms serve distinct roles in transcriptional regulation by partnering with different histone-modifying enzymes. Transcriptional regulation by SIN3 histone deacetylase (HDAC)2 complexes is essential for a number of important biological processes. For instance, SIN3 complexes are required for viability, as demonstrated by the finding that mutations in SIN3 result in embryonic lethality in both Drosophila and mouse (1-4). Furthermore, genome-wide localization and gene expression studies have mapped the SIN3 regulatory network to include nuclear genes involved in mitochondrial biogenesis and function (5), genes involved in DNA replication and repair (6), and genes involved in development (2, 5, 6). Additionally, functional studies in both Drosophila and mammalian systems have shown that SIN3 is an important factor in the regulation of cell cycle progression and exit (2, 3, 7-10). Together, these studies highlight the importance of SIN3 in both growth and development.The SIN3 corepressor serves as a scaffold for the assembly of HDAC complexes. These complexes are recruited to chromatin, where the catalytic subunit, RPD3 in yeast and Drosophila and HDAC1 and -2 in mammals, deacetylates histones to repress transcription (11,12). Compositionally similar SIN3 complexes from Saccharomyces cerevisiae, Schizosaccharomyces pombe, and mammals have been isolated and characterized, illustrating the conservation of SIN3 complex proteins among eukaryotes (11, 13). This similarity further suggests that the essential functions of these complexes may be conserved as well.In yeast, two distinct mechanisms of SI...

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How to get ahead: the origin, evolution and function of bicoid

McGregor

2005

BioEssays

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In Drosophila, a Bcd protein gradient orchestrates patterning along the anteroposterior embryonic axis. However, studies of basal flies and other insects have revealed that bcd is a derived Hox3 gene found only in higher dipterans. To understand how bcd acquired its role in flies and how anteroposterior patterning mechanisms have evolved, I first review key features of bcd function in Drosophila: anterior localization and transcriptional and translation control of gene expression. I then discuss investigations of bcd in other higher dipterans that have provided insight into the evolution of regulatory interactions and the Bcd gradient. Finally, I review studies of Drosophila and other insects that address the evolution of bcd function and integration of bcd into ancestral regulatory mechanisms. I suggest further comparative studies may allow us to identify the intermediate steps in bcd evolution. This will make bcd a paradigm for the origin and evolution of genes and regulatory networks.

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A Composite Motif of the Drosophila Morphogenetic Protein Bicoid Critical to Transcription Control

Cited by 17 publications

References 44 publications

The Co-activator CREB-binding Protein Participates in Enhancer-dependent Activities of Bicoid

The Co-activator CREB-binding Protein Participates in Enhancer-dependent Activities of Bicoid

Drosophila SIN3 Isoforms Interact with Distinct Proteins and Have Unique Biological Functions

How to get ahead: the origin, evolution and function of bicoid

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