volume 47, issue 3, P287-294 2014
DOI: 10.1590/0037-8682-0040-2014
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Abstract: ABSTRACT Introduction: Molecular biology procedures to detect, genotype and quantify hepatitis C virus (HCV) RNA in clinical samples have been extensively described. Routine commercial methods for each specifi c purpose (detection, quantifi cation and genotyping) are also available, all of which are typically based on polymerase chain reaction (PCR) targeting the HCV 5' untranslated region (5'UTR). This study was performed to develop and validate a complete serial laboratory assay that combines real-time nest…

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