A complementary approach to identifying and assessing the remediation potential of hydrocarbonoclastic bacteria

Kadali, Krishna K.; Simons, Keryn L.; Skuza, Pawel; Moore, R. G.; Ball, Andrew S.

doi:10.1016/j.mimet.2011.12.006

Cited by 56 publications

(38 citation statements)

References 54 publications

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“…Cluster analysis, which orders samples according to their similarity indices, is commonly used to show the differences or classification between groups of clusters (Kadali et al, 2012). To determine the number of clusters on the dendrogram (Fig.…”

Section: Sscp Analysis Of Pcr Products and Statistical Analysismentioning

confidence: 99%

Response of soil microorganisms to radioactive oil waste: results from a leaching experiment

et al. 2015

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Section: Sscp Analysis Of Pcr Products and Statistical Analysismentioning

confidence: 99%

Response of soil microorganisms to radioactive oil waste: results from a leaching experiment

et al. 2015

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“…As widely reported, the application of this technique leads to the isolation only of around 0.001% of the microbial population present in sea water and 0.3% present in soil [2,3]. In terms of commercial bioremediation and the management of a bioremediation event, this technology has only been of limited value due to the length of time required for isolation [4]; on many occasions by the time a drop in hydrocarbonoclastic organisms has been observed [5], the bioremediation has already stalled.…”

Section: Introductionmentioning

confidence: 93%

“…However, these species represented various identities (accession number) and similarities in each treatment confirming the fact that they are certainly unique in nature (Table 1). In this present study RNA-TGGE was carried out using universal bacterial primers rather than specific primers, because the site to be remediated contains multiple types of compounds or contaminants [2,32] which is not ideal for the application of specific primers. In this study the contaminant was weathered crude oil, representing a complex mixture of tens of thousands of compounds [31,[33][34][35].…”

Section: Rna-tgge Bandsmentioning

confidence: 99%

RNA-TGGE, a Tool for Assessing the Potential for Bioremediation in Impacted Marine Ecosystems

Kadali

Shahsavari

Simons

et al. 2015

JMSE

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Cultivation-independent genomic approaches have greatly advanced our understanding of the ecology and diversity of microbial communities involved in biodegradation processes. However, much still needs to be resolved in terms of the structure, composition and dynamics of the microbial community in impacted ecosystems. Here we report on the RNA activity of the microbial community during the bioremediation process using RNA Temperature Gradient Gel Electrophoresis (RNA-TGGE). Dendrograms constructed from similarity matching data produced from the TGGE profiles separated a community exhibiting high remediation potential. Overall, increased Shannon Weaver Diversity indices (1-2.4) were observed in the high potential remediation treatment samples. The functionality of the microbial community was compared, with the microbial community showing the greatest organisation also showing the highest levels of hydrocarbon degradation. Subsequent sequencing of excised bands from the microbial community identified the presence of Gammaproteobacteria together with a number of uncultured bacteria. The data shows that RNA TGGE represents a simple, reproducible and effective OPEN ACCESSJ. Mar. Sci. Eng. 2015, 3 969 tool for use in the assessment of a commercial bioremediation event, in terms of monitoring either the natural or augmented hydrocarbon-degrading microbial community.

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“…These enzymes include several dioxygenases, 1-acenaphthenol dehydrogenases, and salicylaldehyde dehydrogenases [46]. On the other hand, the genus Pseudomonas are also commonly present in hydrocarbon-contaminated environments [47][48][49] and their metabolism includes the degradation of various aliphatic and aromatic hydrocarbons [9,12,[50][51][52]. Both Pseudomonas and Acinetobacter strains are also well known to produce a wide variety of extracellular emulsifiers towards improving the bioavailability of hydrocarbons [53][54][55][56][57].…”

Section: Removal Of Tph During Bioremediationmentioning

confidence: 99%

Assessing Technical and Economic Feasibility of Complete Bioremediation for Soils Chronically Polluted with Petroleum Hydrocarbons

Orellana¹,

Cumsille²,

Rojas³

et al. 2017

J Bioremediat Biodegrad

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Petroleum hydrocarbons are highly persistent in the environment and represent a significant risk for humans, biodiversity, and the ecosystems. Frequently, hydrocarbon-contaminated sites remain polluted for decades due to a lack of proper decontamination treatments. Although bioremediation techniques have gained attention for being environmentally friendly, cost-effective and applicable in situ, their application is still limited. Each polluted soil has particularities, therefore, the bioremediation approach for a contaminated site is unique. Bioremediation cost studies are usually based on hypothetical assumptions rather than technical or experimental data. The research aims of this study were to clean-up chronically hydrocarbon-polluted soils using aerobic and anaerobic bioremediation techniques and to carry out an economic evaluation of the most promising bioremediation treatments. The results showed that aerobic biostimulation with vermicompost and aerobic bioaugmentation plus air venting were the most effective treatments, degrading 78% and 73% of total petroleum hydrocarbons (TPH) in chronically hydrocarbonpolluted soils after six weeks, respectively. In contrast, no significant degradation of hydrocarbon was observed by anaerobic biostimulation treatments with lactate and acetate. An economic evaluation of the aerobic treatments were carried out. This analysis revealed that the cost of treating one cubic meter of soil by biostimulation is US$ 59, while bioaugmentation costs US$77. This study provides a clear structure of costs for both aerobic bioremediation approaches based on projections made from these lab-scale incubations. These values represent the first step towards a better understanding of the feasibility of such treatments at larger scales, which is crucial to move on industrial bioremediation of soils chronically polluted with petroleum hydrocarbons.

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A complementary approach to identifying and assessing the remediation potential of hydrocarbonoclastic bacteria

Cited by 56 publications

References 54 publications

Response of soil microorganisms to radioactive oil waste: results from a leaching experiment

Response of soil microorganisms to radioactive oil waste: results from a leaching experiment

RNA-TGGE, a Tool for Assessing the Potential for Bioremediation in Impacted Marine Ecosystems

Assessing Technical and Economic Feasibility of Complete Bioremediation for Soils Chronically Polluted with Petroleum Hydrocarbons

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