A competitive immunochromatographic assay for testosterone based on electrochemical detection

Inoue, Kumi Y.; Ferrante, Pascal; Hirano, Yu; Yasukawa, Tomoyuki; Shiku, Hitoshi; Matsue, Tomokazu

doi:10.1016/j.talanta.2007.05.008

Cited by 46 publications

(34 citation statements)

References 15 publications

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“…Several attempts have been made to increase the signal by introducing an enzyme as the label [36,84,85] or electrochemical signal generation [86][87][88]. Silver enhancement of the response of the gold nanoparticles has been mentioned as well for sensitivity increase [89][90][91].…”

Section: Weaknessesmentioning

confidence: 99%

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

2008

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Lateral flow (immuno)assays are currently used for qualitative, semiquantitative and to some extent quantitative monitoring in resource-poor or non-laboratory environments. Applications include tests on pathogens, drugs, hormones and metabolites in biomedical, phytosanitary, veterinary, feed/food and environmental settings. We describe principles of current formats, applications, limitations and perspectives for quantitative monitoring. We illustrate the potentials and limitations of analysis with lateral flow (immuno)assays using a literature survey and a SWOT analysis (acronym for "strengths, weaknesses, opportunities, threats"). Articles referred to in this survey were searched for on MEDLINE, Scopus and in references of reviewed papers. Search terms included "immunochromatography", "sol particle immunoassay", "lateral flow immunoassay" and "dipstick assay".

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Section: Weaknessesmentioning

confidence: 99%

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

2008

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“…Recently, electrochemical detection systems have been incorporated in immunochromatographic assays for the quantitative determination of target molecules. However, labels, such as nanoparticles, 11 quantum dot [12][13][14][15] and enzymes, [16][17][18][19][20] captured at the line with immobilized by immunorecognition reactions have to be transferred to the electrochemical cell by cutting the membrane in order to convert the labels into electrochemical currents. The use of a strong acid to dissolve the metal particles and a relatively long time for enzyme reaction are unsuitable for practical use by medical practitioners and general patients.…”

Section: Introductionmentioning

confidence: 99%

Quantitative and Single-step Enzyme Immunosensing Based on an Electrochemical Detection Coupled with Lateral-flow System

et al. 2017

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A single-step electrochemical immunochromatography has been developed: the device was based on two pieces of nitrocellulose membrane, a sample pad with anti-mouse IgG antibody labeled with glucose oxidase (GOx-labeled antibody), a conjugate pad with glucose, and a Pt working electrode. Either antibody or antigen was immobilized on the membrane. The addition of a solution containing mouse IgG, a model target, allows for the dissolution of GOx-labeled antibody in the sample pad to form an immunocomplex. The produced immunocomplex was automatically separated by capturing to the antibody immobilized on the membrane with the sandwich structure or by passing through the membrane modified with an antigen for the competitive reaction. The separated GOx label arrived at the conjugate pad with glucose to undergo the enzyme reaction. Hydrogen peroxide generated by this reaction was detected at the Pt electrode prepared on the second nitrocellulose membrane downstream from the conjugate pad. The results demonstrated that the designed immunochromatography can be applied to quantitative detection with a single-step procedure, because both the GOxlabeled antibody for revealing the immunoreactions and the substrate for the enzyme reaction were prepared in the device. Moreover, the initial concentration of the GOx-labeled antibody permitted control of the detectable concentration for mouse IgG.

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“…The measurement principle of the quantitative IST using CA is an oxidation-reduction reaction occurring on the modified electrodes underneath the detection area. 30,31 The measurement principle using EIS is to measure the capacitance on a color line using the modified electrodes with polymer layers. 32,33 However, both methods using CA and EIS are costly because they need additional factors such as enzymes, mediators or polymers in addition to electrodes.…”

Section: Introductionmentioning

confidence: 99%

Impedance Analysis of Colloidal Gold Nanoparticles in Chromatography Paper for Quantitation of an Immunochromatographic Assay

et al. 2016

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A detection method of gold nanoparticles in chromatography paper has been developed for a simple, cost-effective and reliable quantitation of immunochromatographic strip test. The time courses of the solution resistance in chromatography paper with the gold nanoparticles solution are electrochemically measured by chrono-impedimetry. The dependence of the solution resistance on the concentration of gold nanoparticles has been successfully observed. The main factor to increase the solution resistance may be obstruction of the ion transport due to the presence of gold nanoparticles. The existence of gold nanoparticles with 1.92 × 10 9 particles/mL in an indistinctly-colored chromatography paper is also identified by a solution resistance measurement. This indicates that the solution resistance assay has the potential to lower the detection limit of the conventional qualitative assay.Keywords Immunochromatography, chromatography paper, gold nanoparticles, solution resistance

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A competitive immunochromatographic assay for testosterone based on electrochemical detection

Cited by 46 publications

References 15 publications

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

Lateral flow (immuno)assay: its strengths, weaknesses, opportunities and threats. A literature survey

Quantitative and Single-step Enzyme Immunosensing Based on an Electrochemical Detection Coupled with Lateral-flow System

Impedance Analysis of Colloidal Gold Nanoparticles in Chromatography Paper for Quantitation of an Immunochromatographic Assay

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