A Competitive Endogenous RNA Network Based on Differentially Expressed lncRNA in Lipopolysaccharide‐Induced Acute Lung Injury in Mice

Jia, Xianxian; Huang, Jinhui; Wu, Bo; Yang, Miao; Xu, Wei

doi:10.3389/fgene.2021.745715

Cited by 6 publications

(4 citation statements)

References 87 publications

(102 reference statements)

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“…Among the screened lncRNAs, the study identified five different lncRNAs (LNC_006493, RP23-462P13.1, RP23-36001.1, RP24-150D8.2, and RP24-79P8.1) that have critical roles in ALI. These lncRNAs can downregulate Nkx2-1 and act as ceRNAs for the overexpressed mmu-miR-135b-3p [ 122 ].…”

Section: Lncrnas In Ali/ardsmentioning

confidence: 99%

Long Noncoding RNA: A Novel Insight into the Pathogenesis of Acute Lung Injury

Dutta

Zhu²,

Han³

et al. 2023

JCM

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Acute lung injury (ALI) and its severe form, acute respiratory distress syndrome (ARDS), represent an acute stage of lung inflammation where the alveolar epithelium loses its functionality. ALI has a devastating impact on the population as it not only has a high rate of incidence, but also has high rates of morbidity and mortality. Due to the involvement of multiple factors, the pathogenesis of ALI is complex and is not fully understood yet. Long noncoding RNAs (lncRNAs) are a group of non-protein-coding transcripts longer than 200 nucleotides. Growing evidence has shown that lncRNAs have a decisive role in the pathogenesis of ALI. LncRNAs can either promote or hinder the development of ALI in various cell types in the lungs. Mechanistically, current studies have found that lncRNAs play crucial roles in the pathogenesis of ALI via the regulation of small RNAs (e.g., microRNAs) or downstream proteins. Undoubtedly, lncRNAs not only have the potential to reveal the underlying mechanisms of ALI pathogenesis but also serve as diagnostic and therapeutic targets for the therapy of ALI.

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Section: Lncrnas In Ali/ardsmentioning

confidence: 99%

Long Noncoding RNA: A Novel Insight into the Pathogenesis of Acute Lung Injury

Dutta

Zhu²,

Han³

et al. 2023

JCM

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“…GAS5 is downregulated in childhood pneumonia, acute lung injury, and asthma, and its overexpression can cause the inhibition of in ammation and epithelial cell apoptosis [17][18][19] . In our previous genomic study on lipopolysaccharideinduced acute lung injury, the expression of GAS5 was signi cantly altered [20] , but its expression pattern in BPD was unclear. Through a bioinformatics analysis, we found several microRNAs (miRNAs) that coexisted with GAS5 and Sox9, which may constitute a competing endogenous RNA regulatory pathway.…”

Section: Introductionmentioning

confidence: 97%

Role of Sox9 in BPD and its effects on the Wnt/β-catenin pathway and AEC-II differentiation

Bai

et al. 2023

Preprint

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The excessive activation of the Wnt/β-catenin signaling pathway is an important regulatory mechanism that underlies the excessive proliferation and impaired differentiation of type 2 alveolar epithelial cells (AEC-II) in bronchopulmonary dysplasia (BPD). Sox9 has been shown to be an important repressor of the Wnt/β-catenin signaling pathway and plays an important regulatory role in various pathophysiological processes. We found that the increased expression of Sox9 in the early stages of BPD could downregulate the expression of β-catenin and promote the differentiation of AEC-II cells into AEC-I, thereby alleviating the pathological changes in BPD. The expression of Sox9 in BPD is regulated by long noncoding RNA growth arrest-specific 5. These findings may provide new targets for the early intervention of BPD.

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“…These differentially expressed miRs can exacerbate or mitigate BPD progression (12)(13). A research team also found differential expression of miR-195-5p in the lung tissue of a mouse model of lipopolysaccharide-induced acute lung injury (14). Therefore, it is valuable to study the potential role of miR-195-5p in BPD development.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-195-5p targets neurofibromin 2 to mediate hyperoxia-induced alveolar dysplasia

Bai,

Wu,

Yang

et al. 2024

Preprint

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MicroRNAs (miRs) have been shown to disrupt normal lung development and function by blocking alveolation and vascularization, leading to the development of bronchopulmonary dysplasia (BPD). However, the role of miR-195-5p in BPD has not been reported. Therefore, this study aimed to understand whether the biological role of miR-195-5p in BPD is achieved by targeting neurofibromin 2 (NF2) regulation. Here, we report that miR-195-5p plays a key role in hyperoxia-induced alveolar dysplasia in BPD. First, increased expression of miR-195-5p was detected in the lung tissues of hyperoxia-induced rat models of BPD and neonatal rat lung type II alveolar epithelial cells (AECIIs) exposed to hyperoxic conditions. Second, NF2, a tumor suppressor gene that plays an important role in the proliferation and differentiation of AECIIs, was identified as the specific target of miR-195-5p. Cell-based luciferase analysis revealed that the overexpression of miR-195-5p and its target gene NF2 in lung AECIIs could partially alleviate the impaired differentiation of AECIIs induced by hyperoxia, suggesting that miR-195-5p could hinder the differentiation of AECII into AECI by inhibiting NF2 expression. Finally, animal intervention experiments confirmed that miR-195-5p aggravated alveolar dysplasia in BPD by downregulating NF2 expression. In conclusion, our study confirmed a novel mechanism wherein miR-195-5p exacerbates alveolar dysplasia in BPD by inhibiting AECII differentiation to AECI by targeting NF2. These results may help clarify some molecular mechanisms of the poor proliferation and differentiation of AECII in BPD alveolar dysplasia and provide novel targets for future clinical molecular interventions.

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A Competitive Endogenous RNA Network Based on Differentially Expressed lncRNA in Lipopolysaccharide‐Induced Acute Lung Injury in Mice

Cited by 6 publications

References 87 publications

Long Noncoding RNA: A Novel Insight into the Pathogenesis of Acute Lung Injury

Long Noncoding RNA: A Novel Insight into the Pathogenesis of Acute Lung Injury

Role of Sox9 in BPD and its effects on the Wnt/β-catenin pathway and AEC-II differentiation

MicroRNA-195-5p targets neurofibromin 2 to mediate hyperoxia-induced alveolar dysplasia

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