A competent bidrug loaded water soluble chitosan derivative for the effective inhibition of breast cancer

Abstract

Drug resistance and damage caused to the normal cells are the drawbacks which have limited the use of the existing effective anticancer drugs. Attainment of a steady and extended release by encapsulating dual drugs into biocompatible and biodegradable vehicles is the key to enable the use of these drugs for effective inhibition of cancer. In this study, carboxymethyl chitosan (CMCS), a proficient water-soluble derivative of chitosan has been synthesized using chemical route and used for the delivery of 5-Fluorouracil and doxorubicin individually as well as in combination. Carboxymethylation occuring at –NH2 and OH sites of chitosan, has been confirmed using FTIR. EDX and Fluorescence studies elucidate the encapsulation of 5-Fluorouracil and doxorubicin into CMCS. The capability of CMCS to release the drugs in a more sustained and prolonged manner is evident from the obtained release profiles. About 14.9 µg/ml is enough to cause 50% cell death by creating oxidative stress and effectuating DNA fragmentation. Amidst the existing reports, the uniqueness of this work lies in using this rare coalition of drugs for the suppression of breast cancer and in reducing the side effects of drugs by encapsulating them into CMCS, which is evidenced by the high hemocompatibilty of the samples.

Dual ionotropic crosslinked biopolymer magnetic nanocomposites as 5‐Fluorouracil nanocarrier for potential colorectal cancer treatment

“…Figure 5C, the presence of 45.64% oxygen, 25.91% carbon, 4.14% nitrogen, 14.42% phosphorous, and 9.81% calcium provided evidences that Cs, Alg and their respective crosslinkers are present in the NCs 56–58 . Similar to Cs‐Alg/IO 5FU NCs, all elements were present with the addition of 2.23% iron, and fluorine were present in both NCs indicating that the 5FU was successfully loaded into the IPN of NCs 59,60 …”

“…[56][57][58] Similar to Cs-Alg/IO 5FU NCs, all elements were present with the addition of 2.23% iron, and fluorine were present in both NCs indicating that the 5FU was successfully loaded into the IPN of NCs. 59,60 3.4 | Particle sizes, zeta potential and PDI…”

In vitro and functional investigation reveals the curative effect of thymoquinone from black cumin-loaded chitosan nanoparticles on streptozotocin induced paediatric diabetes

“…Maximum frequencies for aliphatic C–H stretching are reported by Nivethaa et al. [ 36 ] to be 2900 cm −1 , for N–H bending to be 1662 cm −1 , for primary alcoholic C–O stretching to be 1414 cm −1 , and for hydroxyl group C3 stretching to be 1098 cm −1 . Tq-CsNPs size distribution as a function of reported intensity.…”

Cellulose Nanofiber-Based Hydrogels Embedding 5-FU Promote Pyroptosis Activation in Breast Cancer Cells and Support Human Adipose-Derived Stem Cell Proliferation, Opening New Perspectives for Breast Tissue Engineering

“…The findings of those studies indicated that 5-FU loaded into polymers was effective at inhibiting MCF-7 breast cancer cells’ growth and proliferation, albeit in a concentration-dependent manner. Additionally, 5-FU and doxorubicin drug encapsulation displayed high cytotoxicity, suggesting the possibility of combinations of drugs in the treatment of multiple types of cancer, including BC [ 38 ]. Christensen et al stated that TP53-mutated BC accumulated more 5-FU mutations than TP53 wild type BC [ 39 ].…”