Parthenium hysterophorus is one of the world's noxious weed and commonly known as congress grass and carrot weed. Parthenium is fast spreading biological pollutant due to its high germination ability throughout the year, large number of seed production, high survival rate, extreme adaptability in wide range of habitats and easy dispersal of seeds. Mycobiota associated with weeds provide essential data for selection of potential biocontrol agent for weed management. Therefore, in the present study fungi associated with different parts of Parthenium were isolated and identified. Total of 35 fungal species belonging to 24 different genera were recorded from leaf, stem, flower, and seed of Parthenium. Maximum number of fungi were obtained from leaf (31) followed by seed (29), flower (25) and stem (15).