The hydro-distilled essential oil of Citrus reticulata Blanco var. kinnow was analyzed by Gas chromatography-mass spectrometry (GC-MS). Five constituents out of fifteen constituents were identified from seeded C. reticulata oil representing 74.66% of the oil. The major constituent of the oil was β-phellandrene (62.00%). β-pinene(6.53%), β-myrcene(2.81%), limonene(2.81%) and caryophyllene(0.51%) were present in considerable amount. From the low seeded C. reticulata oil, six components out of seventeen compounds were identified constituting 54.74% of the oil and the main component was β-phellandrene (37.35%). α-pinene(2.79%), β-pinene(3.26%), β-myrcene(4.16%), limonene(5.77%), caryophyllene(1.41%) were present in considerable amount.