A comparison of two strains of the anaerobic ciliate Trimyema compressum

Monoxenic cultivation of the anaerobic amoeboflagellate Psalteriomonas lanterna and isolation of the methanogenic endosymbiont

“…No growth occurred either when P. lanterna was transferred to the medium used for growth of monoxenic Trimyema compressmn, with Bacteroides sp. strain WoCb as food bacterium [13,14]. However, growth of P. hmterna occurred in MYG medium supplemented with pseudomonas fluorescens.…”

Oxygen responses of the free-living anaerobic amoeboflagellate Psalteriomonas lanterna

“…Catalase activity was demonstrated by the trz.nsfer of a dense cell suspension to a drop of 3% aqueous hydrogen peroxide. Superoxide dismutase (SOD) activity was determined by illumination of the amoeboflageilates in a reaction mixture containing riboflavin and nitro blue tetrazolium salt according to Goosen et al [16].…”

“…When a dense suspension of syrnbiont-free flagellate cells was added to a 3% (v/v) H20: solution, no oxygen production was observed indicating the absence of catalase activity. For the demonstration of SOD, cells were illuminated for 45 min in the reaction mixture according to Goosen et al [16]. In symbiont-free ceils, cultured under an atmosphere of I-2% O z, Table 2 Effect of the addition of bromoethanesulfonic acid (BrES) on growth of P. lanterna cells, methane excretion and I-12 production…”

The effect of endosymbiotic methanogens on the growth and metabolic profile of the anaerobic free-living ciliate Trimyema compressum

“…Some ciliates are known to be anaerobic protozoa which possess hydrogenosomes and endosymbiotic methanogens in the cytoplasm instead of mitochondria [1–6]. The hydrogenosome is known as the exclusive organelle which ferments pyruvate with substrate‐level phosphorylation and hydrogen generation.…”