A comparison of three tests for rubella antibody screening.

Morgan‐Capner, P.; Pullen, H.; Pattison, J. R.; Bidwell, D. E.; Bartlett, A.; Voller, A.

doi:10.1136/jcp.32.6.542

Cited by 26 publications

(8 citation statements)

References 6 publications

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“…The technique of single radial haemolysis (SRH) was employed as described by Morgan-Capner et al (1979). Results were recorded as 'immune' if the diameter of the zone of haemolysis was greater than, or equal to.…”

Section: Measurement Of Antibodies Against Rubellamentioning

confidence: 99%

Immunity to rubella among women of child‐bearing age

Lever

Ross

Baboonian

et al. 1987

BJOG

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The results of testing for rubella antibodies in over 6000 sera from women of child-bearing age are reported and analysed according to pregnant state, age, country of origin and social class. There was no difference between the rubella seroprevalence rates in women who were pregnant and in those who were contemplating pregnancy in the future. Likewise, women (either pregnant or non-pregnant) who were young enough to have been offered rubella vaccine at school were not more likely to be immune to rubella than were older women. Rubella seropositivity rates were not influenced by social class but significantly higher rates were found in women born in European or Arabian than in African or Asian countries. We conclude that the national scheme for rubella immunization has not reduced the number of women susceptible to rubella entering pregnancy in this Health District and that greater attention should be paid to immunization of women of child-bearing age from African or Asian countries.

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Section: Measurement Of Antibodies Against Rubellamentioning

confidence: 99%

Immunity to rubella among women of child‐bearing age

Lever

Ross

Baboonian

et al. 1987

BJOG

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“…For the rubella serosurvey among pregnant women, the Bristol Public Health Laboratory tested for rubella-specific IgG with single radial hemolysis. 32,33 For single radial hemolysis test results of less than 15 IU/mL, the Rubalex latex agglutination test (Abbott Laboratories, Maidenhead, United Kingdom) was used to retest.…”

Section: Rubella Serologic Testingmentioning

confidence: 99%

Unseen blindness, unheard deafness, and unrecorded death and disability: congenital rubella in Kumasi, Ghana

Lawn

Reef²,

Baffoe-Bonnie³

et al. 2000

Am J Public Health

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OBJECTIVES: Although rubella serosusceptibility among women of reproductive age in West Africa ranges from 10% to 30%, congenital rubella syndrome has not been reported. In Ghana, rubella immunization and serologic testing are unavailable. Our objectives were to identify congenital rubella syndrome cases, ascertain rubella antibody seroprevalence during pregnancy, and recommend strategies for congenital rubella syndrome surveillance. METHODS: Congenital rubella syndrome cases were identified through prospective surveillance and retrospective surveys of hospital records. A rubella serosurvey of pregnant urban and rural women was performed. RESULTS: Eighteen infants born within a 5-month period met the congenital rubella syndrome case definitions, coinciding with a 9-fold increase in presentation of infantile congenital cataract. The congenital rubella syndrome rate for this otherwise unrecorded rubella epidemic was conservatively estimated to be 0.8 per 1000 live births. A postepidemic rubella immunity rate of 92.6% was documented among 405 pregnant women; susceptibility was significantly associated with younger age (P = .000) and ethnicity (northern tribes, P = .024). CONCLUSIONS: Congenital rubella syndrome occurs in Ghana but is not reported. Information about congenital rubella syndrome and rubella in sub-Saharan Africa is needed to evaluate inclusion of rubella vaccine in proposed measles control campaigns.

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“…Sixty of these sera were also tested for the presence of CF antibodies against measles virus, respiratory syncytial virus, adenovirus and Mycoplasma pneumoniae. For the measurement of antibodies against rubella virus, sera from 21 of the pregnant women described above were randomized with 19 similar sera known not to contain rubella-specific antibodies detectable by single radial haemolysis (Morgan-Capner et al 1979). …”

Section: Serum Samplesmentioning

confidence: 99%

Hotting-up the complement-fixation test

Hardiman

Griffiths

1983

J. Hyg.

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SUMMARYA detailed investigation into the effect of modifying the incubation temperature of the complement-fixation (CF) test is described. For varicella-zoster virus cytomegalovirus and rubella virus, increasing the incubation temperature progressively increased the sensitivity of the CF test to reach a maximum at 15 0, at which temperature the geometric mean titre of seropositive samples was significantly greater than that found at 40C. For these three viruses, each serum shown to contain IgG antibodies by ultrasensitive radioimmunoassay procedures was detected by CF following incubation at 15 0C. No false-positive reactions occurred at 15 'C, but it was our impression that anticomplementary activity was enhanced at this temperature. Significant increases in antibody titre at 15 'C were also seen when measles virus, respiratory syncytial virus, adenovirus and Mycoplasma pneumoniae were employed as CF antigens. The results demonstrate that the CF test should be performed at 15 0C if optimum sensitivity is to be achieved. The ability ofthe test to detect significant rises in antibody titre was not impaired at the higher incubation temperature.

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A comparison of three tests for rubella antibody screening.

Cited by 26 publications

References 6 publications

Immunity to rubella among women of child‐bearing age

Immunity to rubella among women of child‐bearing age

Unseen blindness, unheard deafness, and unrecorded death and disability: congenital rubella in Kumasi, Ghana

Hotting-up the complement-fixation test

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