A comparison of three mucus-secreting airway cell lines (Calu-3, SPOC1 and UNCN3T) for use as biopharmaceutical models of the nose and lung

Abstract

Graphical abstract

The Path from Nasal Tissue to Nasal Mucosa on Chip: Part 2—Advanced Microfluidic Nasal In Vitro Model for Drug Absorption Testing

“…Thus, the lower TEER values in the static permeation studies resulted in higher permeation coefficients. The impact of the TEER value on the permeation coefficient of FD4 (fluorescein isothiocyanate dextran with an average molecular weight of 4000 Da) on mucus-secreting airway cell lines has been reported by Lee et al [51].…”

Impact of mucus modulation by N-acetylcysteine on nanoparticle toxicity

“…3 F). Several gene expression studies have reported the expression of both MUC5AC and MUC5B in Calu-3 cells ( Gupta et al, 2019 ; Lee et al, 2021 ). Sonntag et al (2022) assessed mucus production in Calu-3 cells by evaluating the level of MUC5AC formation.…”

The effect of ingredients commonly used in nasal and inhaled solutions on the secretion of mucus in vitro

“…This study examined the effect of the corticosteroid, FF on mucus secretion. Despite agreement on the need to investigate the long-term effects of IN steroids on the nasal mucosa, research to date has reported significant improvement of compromised mucociliary function in patients with allergic rhinitis (Hofmann et al, 2004;Houtmeyers et al, 1999a;Klossek et al, 2001;Lee and Gendeh, 2003;Naclerio et al, 2003;Verret and Marple, 2005). However, little is known of the effect of corticosteroid IN formulations on airway mucin secretion.…”

“…The use of mucin-secreting cell culture models is an area of research that bears much potential (Lechanteur et al, 2018). Both human bronchial epithelial primary cells (Abdullah et al, 2012) (Hill and Button, 2012;Kemp et al, 2004) and cell lines e.g., UNCN3T, Calu-3 and SPOC1 (rat) have successfully demonstrated mucin secretion (Abdullah et al, 2003;Abdullah and Davis, 2007;Abdullah et al, 1996;Fulcher et al, 2005;Lee et al, 2021;Randell et al, 1996). However the culture and differentiation procedures for primary cultures are labour-intensive and cell lines rarely express motile cilia.…”